Identification scheme for G+ bacteria

Question 1

Cocci, Aerobic, Catalase-

Answer 1

Streptococcus

Question 2

Cocci, Aerobic, Catalase-, bile soluble or optochin sensitive

Answer 2

S. pneumoniae

Question 3

Cocci, Aerobic, Catalase-, not bile soluble nor optochin sensitive

Answer 3

Streptococcus group D

Question 4

Cocci, Aerobic, Catalase-, not bile/optochin sensitive, bile esculin sensitive, but is hemolysis (alpha)

Answer 4

Viridans groups

Question 5

Cocci, Aerobic, Catalase-, not bile/optochin sensitive, bile esculin sensitive, but is hemolysis (beta) and bacitracin sensitive

Answer 5

Group A Strep

Question 6

Cocci, Aerobic, Catalase-, not bile/optochin sensitive, bile esculin sensitive, but is hemolysis (beta), not bacitracin sensitive and hydrolyzes Hippurate/cAMP

Answer 6

Group B Strep

Question 7

Cocci, Aerobic, Catalase-, not bile/optochin sensitive, bile esculin sensitive, but is hemolysis (beta), not bacitracin sensitive and doesn’t hydrolyzes Hippurate/cAMP

Answer 7

Other Lancefield groups (strep)

Question 8

Cocci, Aerobic, Catalase-, not bile/optochin sensitive, but bile esculin sensitive and contains 6.5% NaCl

Answer 8

Enterococcus

Question 9

Cocci, Aerobic, Catalase-, not bile/optochin sensitive, but bile esculin sensitive and doesnt contains 6.5% NaCl

Answer 9

Nonenterococcus

Question 10

Cocci, Aerobic, Catalase+, Doesn’t ferment glucose

Answer 10

Micrococcus

Question 11

Cocci, Aerobic, Catalase+, ferments glucose

Answer 11

Staphylococcus

Question 12

Cocci, Aerobic, Catalase+, ferments glucose and coagulase+

Answer 12

S. aureus

Question 13

Cocci, Aerobic, Catalase+, ferments glucose and coagulase- and novobiocin resistant

Answer 13

S. saprophyticus

Question 14

Cocci, Aerobic, Catalase+, ferments glucose and coagulase- and not novobiocin resistant

Answer 14

other coagulase negative species

Question 15

Bacilli, not acid-fast, forms endospores, and aerobic

Answer 15

Bacillus

Question 16

Bacilli, not acid-fast, forms endospores, and anaerobic

Answer 16

Clostridium

Question 17

Bacilli, not acid-fast, doesn’t form endospores, is motile

Answer 17

Listeria monocytogenes

Question 18

Bacilli, not acid-fast, doesn’t form endospores, is not motile, aerobic

Answer 18

Corynebacterium Kurthia

Question 19

Bacilli, not acid-fast, doesn’t form endospores, is not motile, aerobic, but catalase positive

Answer 19

Corynebacterium

Question 20

Bacilli, not acid-fast, doesn’t form endospores, is not motile, aerobic, but catalase negative

Answer 20

Eryspielothrix

Gardnerella

Question 21

Bacilli, not acid-fast, doesn’t form endospores, is not motile, anaerobic

Answer 21

Propionibacterium

Question 22

Bacilli, acid fast

Answer 22

Mycobacterium

Nocardia