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HISTOPATHOLOGY > HP - TEMP & NUMBERS > Flashcards

HP - TEMP & NUMBERS Flashcards

1
Q

temp of WATER BATH?

A

45-50°C / 6-10°C LOWER than the MP of wax

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2
Q

temp for Rapid Substitution/Rapid Freezing

A

-160 to -180°C

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3
Q

temp of Paraffin Oven during INFILTRATION?

A

2-5°C ABOVE the MP of wax

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4
Q

temp of Paraffin Oven during EMBEDDING?

A

5-10°C ABOVE the MP of wax

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5
Q

VOL. OF RGTS?

F?

D?

D?

C?

I?

A

Fixation- 20x

Decal-20x

Dehy-10x

Clearing-10x

Infiltration-25x

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6
Q

Volume of fixative for prolonged fixation

A

50-100x

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7
Q

vol of fixative for COLON POLYP?

A

size of colon polyp is

2 x 2 x 3 cm = 12 cm

12 x 20 = 240 ml of FIXATIVE

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8
Q

Water must be removed by heating the wax at what temp?

A

100-105 deg C

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9
Q

Thickness of tissue section:

Frozen section?

Paraffin section (Rotary/rocking)?

Celloidin section (sliding)?

Semithin?

Ultrathin?

A

Frozen section = 4 micra

Paraffin section (Rotary/rocking)= 4-6 micra

Celloidin section (sliding) = 10-15 micra

Semithin = 0.5 - 1 micra

Ultrathin = 60-100 nm

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10
Q

Temperature of liquid nitrogen

A

-170°C (-160 TO -180°c)

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11
Q

MICROTOME KNIFE

One side of the knife is flat/straight while the other is concave

Base-sledge/Rocking/Rotary microtome

MEASUREMENT?

A

PLANE

(25mm in length)

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12
Q

MICROTOME KNIFE

with both sides concave (ROUTINE PARAFFIN SECTIONS)

Recommended for ROTARY MICROTOME

MEASUREMENT?

A

BICONCAVE

(120mm in length)

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13
Q

MICROTOME KNIFE

have both sides straight (FROZEN SECTIONS and extremely HARD AND TOUGH specimens)

Base-sledge/Sliding microtome

MEASUREMENT?

A

PLANE WEDGE

(100 mm in length)

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14
Q

Most routine cryostat work is performed with chamber temperature of

A

commonly -18 to -20°C (ARC: -20°C)

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15
Q

Refractive index of glass

A

1.518

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16
Q

traditional temp of HP?

A

22-25°C

17
Q

temp of Autotechnicon?

A

40°C

18
Q

✓ Fixation temperature:
✓ Normally used:

A

✓ Fixation temperature: RT (18-30°C)
✓ Normally used: 20-22°C

19
Q
  1. Fixative Osmolality
  2. Hypertonic solutions can cause what?
  3. Isotonic (340 mOsm)/Hypotonic solutions cause what?
  4. Added to Osmium tetroxide fixatives for EM:
A
  1. slightly hypertonic solutions (400-450 mOsm)
  2. shrinkage
  3. swelling and poor fixation
  4. Sucrose
20
Q
  1. Fixative pH:
  2. The Optimum temperature range for fixation is:
  3. It accelerates fixation but hastens autolytic changes and enzyme destruction
A
  1. Fixative pH: Neutral (6.0-8.0)
  2. 37-56°C
  3. Moderate Heat
21
Q

temp:

  1. Tissue processors:
  2. Electron Microscopy and Histochemistry:
  3. Mast cells for EM:
A
  1. 40-42°C
  2. 0-4°C
  3. RT
22
Q

Nucleic acids fixation temp?

A

Rapid at higher temp

23
Q

temp:

Rapid fixation of very urgent biopsy specimens-

A

Formalin heated to 60°C

24
Q

temp:

Fixing tissues with tuberculosis-

A

Formalin heated to 100°C

25
Q
  1. Dehydration is accomplished through the use of:
  2. Starting Concentration:
  3. Final Concentration:
A
  1. increasing grades of alcohol
  2. Starting Concentration: 70%
  3. Final Concentration: 100%
26
Q
  1. Infiltration in overheated paraffin above ____°C produces shrinkage and hardening of tissues
  2. Ideal MP of infiltrating agent:
  3. Normally used for routine work:
A
  1. 60°C
  2. 56-58°C
  3. 56°C (56-60°C)
27
Q

The knife is usually tilted at ____° angulation on a microtome to allow a clearance angle between the cutting facet and the tissue block

A

0-15

28
Q

When specimen is more than a few drops, the fluid should be centrifuged first. This is usually done at moderate speed at _____ RPM for ___ minute(s).

A

2000 RPM, 2 minutes

29
Q

By far the best method to collect cells from body fluid (e.g., urine, pleural or peritoneal fluid):

A

✓ Cytospin Preparation (1000 RPM for 1 minute)

30
Q

vacuum embedding involves wax impregnation under _____ atmospheric pressure. A vacuum pressure of up to ____ is applied using a water or mechanical pump

A

Negative pressure; 760 mmHg

31
Q

recommended temp of decalcification?

A

18-30 C

32
Q

Melting point of the embedding medium that is used as a substitute for paraffin wax and is a mixture of highly purified paraffin wax and synthetic plastic polymers.

A

56-57 C

33
Q

An angle refers to the angle formed between actual cutting edges.?

A

Bevel angle (27-32 deg)

34
Q

This is an angle which ensures maximum penetration of tissues and minimizing distortion.

A

Inclination angle (15°)

HISTOPATHOLOGY (13 decks)

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