How enzymes work 2

Question 1

What is the definition of enzymatic activity?

Answer 1

amount of enzyme required to catalyse the conversion of 1 micro molar substrate per minute (measure of how fast a substrate is converted)

Question 2

What is the definition of specific activity?

Answer 2

amount of total enzyme (mg) required to catalyse the conversion of 1 micro molar substrate per time
(enzyme purity)

Question 3

what are the 3 assumptions of the Michaelis Menten?

Answer 3

1) At high [S] the enzymatic reaction has zero order
2) total enzyme conc is constant
3) steady state assumption

Question 4

What is Vmax & Km?

Answer 4

Max - maximal reaction rate that would be obtained at an infinitely large [S]
Km - [S] at which half the maximum reaction rate is attained