history of microbiology Flashcards

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1
Q

who was the first person to observe and accurately describe bacteria

A

Antony van Leeuwenhoek (1632-1723)

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2
Q

define spontaneous generation

A

the belief that living organisms could develop from nonliving matter

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3
Q

who first opposed the theory of spontaneous generation

A

Francesco Redi

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4
Q

what did francesco redi do to oppose spontaneous generation theory

A

he did some fun experiments with meat in an uncovered jar, covered, and partially covered (permeable layer)

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5
Q

what were the findings of francesco redi’s experiments

A

found that maggots only arose in the uncovered and partially covered flasks = they arose from the eggs of flies that were attracted to the meat rather than the meat itself

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6
Q

who came after francesco redi

A

john needam

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7
Q

what did john needam believe

A

spontaneous generation could occur under the right conditions

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8
Q

describe john needam’s experiment + findings

A

had a broth that was heated, then left open as it cooled, he waited, and then observed growth

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9
Q

who came after john needam

A

lazzaro spallanzani

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10
Q

what did lazzaro spallanzani believe

A

he believed that the growth from john needam’s experiment was due to the flask being left open (ie experimenter error)

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11
Q

describe lazzaro spallanzani’s experiment

A

replicated john needam’s (with open flask) and also did it with a closed flask.

open flask= growth, closed flask=no growth

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12
Q

after all the experiments (redi, needam, spallanzani), what did critics have to say

A

they suggested that heating the air in the sealed flasks destroys the ability of the air to support life

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13
Q

who settled the spontaneous generation controversy

A

louis pasteur

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14
Q

describe pasteur’s experiment

A

poured beef broth into a long-necked flask, bent it into an S-shape, boiled it, then observed that there was no growth even though it was exposed to air

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15
Q

summarize pasteur’s findings

A

microbes are found in nonliving material and they can be destroyed by heat

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16
Q

how is sterility possible

A

microbes are destroyed by heat, so solutions can be kept sterile by heating them and blocking access of microbes to the solution –> aseptic technique

17
Q

what is the process of pasteurization

A

keeping products sterile to make them safe for consumption

18
Q

what is the germ theory of disease and who came up with it

A

microbes might be responsible for causing disease; pasteur

19
Q

what evidence did joseph lister show

A

microbes could cause disease (but this evidence was indirect)

20
Q

describe what joseph lister did

A

he knew that phenol could kill bacteria, so he developed a system of surgery that was designed to prevent microbes from entering wounds (this led patients to have fewer infections)

21
Q

who came up with the first (direct) proof that microbes could cause human disease

A

Robert Koch

22
Q

how did robert koch find the first proof that microbes could cause disease

A

he was trying to find out what caused anthrax. He took material from diseased animals + injected into healthy ones = mice sick. Using a piece of spleen from a sick mouse, he grew the bacteria in beef serum, injected into healthy mouse = anthrax developed

23
Q

what was koch’s greatest accomplishment

A

he found the causative agent of tuberculosis
(it’s a rod-shaped bacterium)

24
Q

how do we determine if an agent is linked to a disease? (ie what is the name for the steps we follow)

A

Koch’s postulates

25
Q

describe koch’s postulates

A
  1. microbe must be present in every case of disease and absent from healthy organisms
  2. suspected microbe must be isolated and grown in pure culture
  3. same disease must occur when microbe is put into healthy host
  4. same microbe must be isolated from the now-sick host