Histopathology Flashcards
Father of Histopathology
Johannes Muller
The founder of pathology and used different methods of fixation. Heat fixation to preserve tissues and other samples
Marcelo Malpighi
He discovered formalin, the most popular fixative
Prof. F Blum
the section in the laboratory primarily focused on the disease diagnosis based on the gross, microscopic, immunohistochemical, and molecular examination of body fluids and solid tissue specimens
Anatomic Pathology
specimens were taken from the morgue and were labeled “A”
Autopsy
specimens are collected from the operating room and were labeled “S”
Biopsy
process of recording the tissue specimen in a logbook and assigning identification numbers to the specimen
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Number
preserving the tissue specimen in a life-like manner as possible. Routine fixative used in the laboratory is 10% formalin
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Fixation
process of removing water from the specimen by using grades of ethyl alcohol
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Dehydration
known as de-alcoholization. Process of removing excess alcohol in the tissues and making the tissues transparent.
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Clearing
Routine clearing agent is _______
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
xylene
process of filling up tissues spaces or cavities using melted paraffin wax.
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
infiltration
process of placing the infiltrated tissue inside a mold.
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Embedding / Molding
Melted ______ is poured over the tissue and allows solidifying
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
paraffin wax
process of removing the excess paraffin wax from the block until it assumes
the shape of a truncated pyramid
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Trimming
also known as microtomy
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Sectioning
A tissue block is cut into thin slices called ribbons/sections using _______
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
microtome
a process that employs the use of hematoxylin and eosin dyes to differentiate the cells and the cellular components
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
staining
process of putting the coverslip on the stained tissue using a mounting medium
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
mounting
means of identifying the specimen. Specimen number is indicated on the glass slide
CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene
Labeling
a diagnostic technique used to investigate superficial masses or lumps
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Fine needle aspiration biopsy (FNAB)
Method of screening for cervical cancer and pre-cancerous changes in the cervix. This procedure
is also done to detect STD’s.
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Papanicolaou smear
paraffin-embedded specimen prepared from dried mucus, sputum and debris found in pleural fluids, pericardial fluids, and other sites that cannot be processed in the usual fashion.
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Cell block
the cytological technique specifically designed to concentrate cells on a slide in a
uniform monolayer using high-speed centrifuge. The monolayer distribution improves the morphological appearance of the cell present
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Cytospin
Performed when an immediate or rapid microscopic analysis of a specimen is needed.
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Frozen section
Consist of biomarkers that are important in the genetic testing for breast cancer. Panels are estrogen receptor assay, progesterone receptor assay, C-erb-2 (Her2-neu), p53 and DNA ploidy analysis
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Breast panel
Employs the use of special stains to determine the chemical compounds and their distribution
within and in between the biological cells of the body
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Special staining (Histochemistry)
process of detecting antigens in the cells of tissue sections by using the principle of antibodies binding to specific antigens.
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Immunohistochemical staining
This procedure is important in the diagnosis of abnormal cells such as cancerous cells
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Immunohistochemical staining
Also known as autopsy or necropsy
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Post mortem examination
This highly specialized surgical procedure consists of a thorough examination of a dead body to determine the cause of death, the manner of death, and to evaluate any disease or injury that may have been present.
CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)
Post mortem examination
Autopsies may be performed for either medicolegal or non-medicolegal purposes. True or False
True
