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PMLS 1 > Histopathology > Flashcards

Histopathology Flashcards

1
Q

Father of Histopathology

A

Johannes Muller

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2
Q

The founder of pathology and used different methods of fixation. Heat fixation to preserve tissues and other samples

A

Marcelo Malpighi

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3
Q

He discovered formalin, the most popular fixative

A

Prof. F Blum

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4
Q

the section in the laboratory primarily focused on the disease diagnosis based on the gross, microscopic, immunohistochemical, and molecular examination of body fluids and solid tissue specimens

A

Anatomic Pathology

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5
Q

specimens were taken from the morgue and were labeled “A”

A

Autopsy

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6
Q

specimens are collected from the operating room and were labeled “S”

A

Biopsy

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7
Q

process of recording the tissue specimen in a logbook and assigning identification numbers to the specimen

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Number

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8
Q

preserving the tissue specimen in a life-like manner as possible. Routine fixative used in the laboratory is 10% formalin

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Fixation

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9
Q

process of removing water from the specimen by using grades of ethyl alcohol

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Dehydration

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10
Q

known as de-alcoholization. Process of removing excess alcohol in the tissues and making the tissues transparent.

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Clearing

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11
Q

Routine clearing agent is _______

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

xylene

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12
Q

process of filling up tissues spaces or cavities using melted paraffin wax.

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

infiltration

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13
Q

process of placing the infiltrated tissue inside a mold.

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Embedding / Molding

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14
Q

Melted ______ is poured over the tissue and allows solidifying

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

paraffin wax

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15
Q

process of removing the excess paraffin wax from the block until it assumes
the shape of a truncated pyramid

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Trimming

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16
Q

also known as microtomy

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Sectioning

17
Q

A tissue block is cut into thin slices called ribbons/sections using _______

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

microtome

18
Q

a process that employs the use of hematoxylin and eosin dyes to differentiate the cells and the cellular components

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

staining

19
Q

process of putting the coverslip on the stained tissue using a mounting medium

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

mounting

20
Q

means of identifying the specimen. Specimen number is indicated on the glass slide

CHOICES:
Clearing, Sectioning, infiltration, Labeling, Fixation, Dehydration, Number, Embedding / Molding, Trimming, staining, mounting, microtome, paraffin wax, xylene

A

Labeling

21
Q

a diagnostic technique used to investigate superficial masses or lumps

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Fine needle aspiration biopsy (FNAB)

22
Q

Method of screening for cervical cancer and pre-cancerous changes in the cervix. This procedure
is also done to detect STD’s.

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Papanicolaou smear

23
Q

paraffin-embedded specimen prepared from dried mucus, sputum and debris found in pleural fluids, pericardial fluids, and other sites that cannot be processed in the usual fashion.

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Cell block

24
Q

the cytological technique specifically designed to concentrate cells on a slide in a
uniform monolayer using high-speed centrifuge. The monolayer distribution improves the morphological appearance of the cell present

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Cytospin

25
Q

Performed when an immediate or rapid microscopic analysis of a specimen is needed.

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Frozen section

26
Q

Consist of biomarkers that are important in the genetic testing for breast cancer. Panels are estrogen receptor assay, progesterone receptor assay, C-erb-2 (Her2-neu), p53 and DNA ploidy analysis

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Breast panel

27
Q

Employs the use of special stains to determine the chemical compounds and their distribution
within and in between the biological cells of the body

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Special staining (Histochemistry)

28
Q

process of detecting antigens in the cells of tissue sections by using the principle of antibodies binding to specific antigens.

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Immunohistochemical staining

29
Q

This procedure is important in the diagnosis of abnormal cells such as cancerous cells

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Immunohistochemical staining

30
Q

Also known as autopsy or necropsy

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Post mortem examination

31
Q

This highly specialized surgical procedure consists of a thorough examination of a dead body to determine the cause of death, the manner of death, and to evaluate any disease or injury that may have been present.

CHOICES:
Post mortem examination, Immunohistochemical staining, Frozen section, Special staining (Histochemistry), Papanicolaou smear, Breast panel, Cell block, Cytospin, Fine needle aspiration biopsy (FNAB)

A

Post mortem examination

32
Q

Autopsies may be performed for either medicolegal or non-medicolegal purposes. True or False

A

True

PMLS 1 (23 decks)

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