Handout15

Question 1

what are the bacteria that are normal flora in the mouth and found in plaque biofilms?

Answer 1

haemophilius
actinobacillus
Eikenella
Capnocytophaga
porphymonas
prevotella
fusobacterium
leptotrichia
wilnella
selomonas
treponema

Question 2

makes a sticky surface for other bugs?

Answer 2

dextrans

dexter is a sticky kind of guy

Question 3

used by bacteria to store energy?

Answer 3

glucans

glucan glue can give you energy! kid’s that eat glue

Question 4

additional storage forms made by bugs-

Answer 4

fructans/levans. when these get broken down the plaque gets more acidic.

Question 5

what are the examplsof polysaccharides that bugs make? they hydrolyze the SUCROSE and make these

Answer 5

dextrans, glucans, fructans/levans.

Question 6

how do bacteria stick to the tooth or plaque to cause caries?

Answer 6

MUST have bugs to get perio disease.
they stick by CA2+ ions to make a bridge between the enamel and pellicle and bug
OR more likely bacteria use their fimbrae, pill, or flawless to bind to the glyocproteins (salivary) or the pellicle

Question 7

what is a biofilm?

Answer 7

biofilm is defined as a complex, functional community of one or more species of microbes, encased in an exopolysaccharide matrix and attached to one another or to a solid surface

Question 8

what is the pioneer group of bacteria made of?

Answer 8

gram + cocci and rods

Question 9

what’s next after the pioneer group?

Answer 9

then you have the gram - cocci and rods (because they’re more anaerobic)

Question 10

what are the last colonizers of the biofilm?

Answer 10

fusobacterium, spirillum, Spirochetes
FSS (FUSS)
they’re most anaerobic!

Question 11

what are the 3 things quorum sensing does for communication?

Answer 11

1) Control genes related to extracellular polysaccharide production (remember, that’s what the bacteria make out of the sucrose)
2) Reduce metabolism (for bacteria at the bottom of the matrix)
3) Control production of virulent factors, including drug-destroying genes
* so they have to do with genes and metabolism*

Question 12

what are the free living organisms called? what are most human infections caused by?

Answer 12

most human infections are from the organisms encased in biofirlms- called sessile.
free living are the planktonic.

Question 13

who starts the plaque! colonizatioN!

Answer 13

streptococi first, then bactericides and then fusobacterium. plaque matures as you see actinomyces!
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