Handout 5 Flashcards
advantages to using molecular techniques for the analysis of infectious agents?
SSS
sensitivity
safety
speed
what is the copy machine for DNA?
PCR
what is restriction fragment length polypmorphism used for? example from class?
the military example of sequencing a strain
finger printing
what is the method that detects PATHOGEN in the DNA sample?
DNA hybridizaiton
what uses a probe to look for pathogen DNA in a fixed sample?
In SITU hybridization
AKA fingerprinting?
RFLP
what are you detecting in serological testing?
ANTIBODY!
soluble molecules forming antibody/antigen complexes?
precipitation
clumping larger antigens like CELLS?
agglutination
what uses antibodies to look for markers on cells/separate cells?
flow cytometry
what uses antibodies to detect antigen?
ELISA
what identifies proteins or antibodies to a protein?
western blot
what is similar to ELISA but uses radiation?
RIA!
what is the microbiological approach that ID based on antigens, DNA or other molecular techniques?
immunological/molecular
what is the old schools approach that uses the 5 Is?
pure microbiological appraoch.
innoculation, incubation, isolation, inspection, identificaiton
