Handout 4 Flashcards
- formalin (37% in water) - denatures proteins and nucleic acids – alkylating agent
formaledhyde
ethylene oxide – for heat sensitive materials
GAS STERILIZATION
1) 2% gluteraldehyde – alkylated DNA and proteins
chemical sterilization
put in the chemiclve with formaldehyde and a little water
Dry heat 320F for 2 hours or 340F for 1 hour. Coagulates proteins – safe for metal will not dull or corrode
heat sterilization
can be used for heat sensitive materials but SLOW (10-16 hours)
ethylene oxide
for heat sensitive materials
is paterurization sterilization or disinfectant?
only a disinfectant!
what time and temp should you use for autoclave?
121 C for 15 - 20 mins at 15 psi
check with bacterial spores
pass through small pores that bacteria are excluded – technically not sterile
filters
UV light – thymine dimers (hoods, food prep areas); gamma (gloves)
radiation
in a _____ culture, multiple organisms grow but look different?
differential
in this culture, organisms are enriched for unique metabolic needs?
specialized
what kind of media will inhibit the growth of some?
selecitve
differential wil alom the ID of certain types of organisms
why might a look at the colony morphology be useful?
might help with ID. circular, spindle, raised, etc.
what color with the hemolysis of ALPHA?
green!
beta hemolysis color
CLEAR_ completely cleared cuz it’s BETTA
