Gram -ve rods - part 2 Flashcards

1
Q

Describe Shigella species in terms of their movement and where they are found.

A

Non-motile

Found in food and water

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2
Q

Describe the critical inoculum of Shigella species.

A

Very low inoculum - <200 bacteria to cause infection

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3
Q

What do Shigella species cause?>

A

Bloody diarrhea, fever, cramps, dysentery

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4
Q

Where would you get Yersinia enterocolitica from?

A

Pork, cold cuts, water

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5
Q

What does Yersinia enterocolitica cause?

A

Diarrhea and pseudoappendicitis syndrome

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6
Q

What is pseudoappendicitis?

A

Yersinia enterocolitica gets into the mesenteric lymph nodes and produces a painful lymphadenopathy which can mimick the presentation of appendicitis

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7
Q

What is the bacterium that caused the black death?

A

Yersinia pestis

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8
Q

What are the two forms of the plague?

A

Bubonic and pneumonic forms

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9
Q

Where does one get yersinia pestis from?

A

Fleas from rats

carried by ground squirrels in the SW USA

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10
Q

What does bubos mean?

A

Lymph nodes of the groin

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11
Q

If someone has the pneumonic form, how can it be transmitted?

A

Via the respiratory route

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12
Q

How does one get the pneumonic form of the plague?

A

Lymph system is connected to the circulation via the thoracic duct which can allow Yersinia pestis to settle in the lungs

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13
Q

What is arteritis obliterans?

A

end of extremities mummify - blackening of extremities associated with the Black death

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14
Q

Describe the movement status of Klebsiella pneumoniae and what is causes.

A

Non motile

Pneumonia, UTIs, sepsis

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15
Q

Klebsiella pneumoniae causes pneumonia mostly in what individuals?

A

Alcoholics, the debilitated and the hospitalized

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16
Q

What does Klebsiella granulomatis cause?

A

Causes granuloma inguinale, an STI

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17
Q

Painless genital ulcers that look like primary syphilis but little inguinal lymphadenopathy.

A

Granuloma inguinale

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18
Q

Klebsiella granulomatis is more common where?

A

Developing countries

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19
Q

This bacterium is a leading cause of nosocomial infections.

A

Serratia marcescens

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20
Q

Very antibiotic resistant bacterium.

A

Serratia marcescens

21
Q

Some strains of this bacterium produce what type of pigment?

A

Blood red pigment (Serratia marcescens)

22
Q

When people get pneumonia at the ICU - these bacterial causes are the ones to worry about.

A

P. aeruginosa

Serratia marcesens

23
Q

How are non-fermenters different from the enterobacteriaceae?

A

they cannot ferment glucose

24
Q

How are non-fermenters distinguished from Enterobacteriaceae?

A

Non-fermenters fail to acidify the butt of a TSI agar or Kliger;s iron agar

25
Q

What is tough about non-fermenters?

A

Tough to treat and identify

26
Q

Non-fermenters constitute what percentage of gram negative isolates?

A

15%

27
Q

What is a key characteristics of non-fermenters in terms of treatment?

A

Very antibiotic resistant - require longer courses of antibiotics then fermentative bacteria

28
Q

75% of non-fermenting gram negative rod isolates belong to what three species?

A

P. aeruginosa
Acinetobacter anitratus
Stenotrophomonas maltophilia

29
Q

Where can P. aeruginosa be found?

A

anywhere that is moist

30
Q

What makes Pseudomonas aeruginosa able to tolerate many conditions?

A

Can live anywhere that is moist
has minimal nutritional requirements
Encapsulated
Antibiotic resistant

31
Q

Describe Pseudomonas aeruginosa’s motility.

A

Polar flagella

32
Q

What shaped colonies does Pseudomonas aeruginosa produce on MacKonkey agar?

A

football shaped colonies

33
Q

What biochemical test could be used to differentiate between P. aeruginosa and A. anitratus + S. maltophilia?

A

P. aeruginosa is cytochrome oxidase positive, the others are not

34
Q

What characteristic odour does Pseudomonas have?

A

Grape-like odour

35
Q

Why are colonies usually seen as green?

A

Pyocyanin and pyoverdin pigments (blue and yellow, respectively) combine to form green

36
Q

Surprisingly, what can P. aeruginosa contaminate?

A

Sterile solutions and even disinfectants

37
Q

Pseudomonas infections are mostly of what type?

A

Nosocomial

38
Q

P. aeruginosa is very invasive, how does it travel?

A

Along blood vessel walls.

39
Q

Black coloured cellulitis that spreads fast along blood vessels.

A

Ecthyma gangrenosum

40
Q

What are the different nosocomial infections caused by P. aeruginosa?

A

Pneumonia, UTIs, surgical wound infections, infectious of burns
ecthyma gangrenosum

41
Q

What are the different community acquired infections due to P. aeruginosa?

A

Malignant otitis externa (glue ear)
Hot tub folliculitis
Endocarditis
Osteomyelitis of calcaneum

42
Q

Endocarditis due to P. aeruginosa occurs in what people?

A

IV drug abusers

43
Q

Malignant otitis externa mainly occurs to which individuals?

A

Type 1 diabetics

44
Q

Osteomyelitis of calcaneum usually occurs after what type of injury?

A

Penetrating injury

45
Q

Similarly to P. aeruginosa, Acinetobacter species can also contaminate what?

A

sterile solutions and even disinfectants

46
Q

Stenotrophomas maltophilia tends to be isolated from what types of people?

A

Those on broad spectrum antibiotics - especially carbapenems

47
Q

If this bacterium is overgrowing in the ICU, it indicates that carbapenem antibiotics are being overused.

A

Stenotrophomas maltophilia

48
Q

This bacterium caused the closure of CF camps.

A

Burkoldaria cepacia (very resistant strains being passed on)

49
Q

What does Burkoldaria cepacia cause?

A

Respiratory infections in CF patients.