Genetische Mechanismen Flashcards

1
Q

sigma Faktor

A

Beginn der bakeriellen Transkrtiption
erkennt Pribnow Box/ -10 und -35

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2
Q

Termination der Transkription

A

rho-unabhängig: inverted repeats, Schleifen-Bildung, Poly-U
rho-abhängig: Proteinfaktor, beindet an mRNA, wandert zur Polymerase, die stoppt und sich ablöst

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3
Q

Hemmung der Translation: Chlroamphenicol

A

hemmt 50S UE, Verlöngerung

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4
Q

Hemmung der Translation: Tetracyclin

A

hemmt 30S UE, Verlöngerung

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5
Q

Hemmung der Translation: Streptomycin

A

hemmt 30 S UE, Start

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6
Q

Überspiralisierung

A

rechts: negativ
links: positiv
Topoisomerase II/Gyrase macht negativ
Topoisomerase I entspiralisert
Histone negativ überspiralisiert

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7
Q

RNA Polymerase von E.coli

A

5 verschiedene Protein-Untereineheiten
sigma-Faktor nicht fest gebunden, erkennt geeignete Start-Stelle
Core Enzym allein kann RNA Bildung katalysieren

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8
Q

Archaeelee Transkritpion

A

-33 BRE
-25 TATA-Box
Bindung von Proteinen an BRE und TATA für Bidung der RNAPol an Inr nätig

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9
Q

Inhibitoren der RNA Polymerase: Rifamycin

A

Angriff an beta-UE der RNA-Pol

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10
Q

Inhibitoren der RNA Polymerase: Streptolydigin

A

bindet beta-UE der RNA Pol

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11
Q

Inhibitoren der RNA Polymerase: synthetische Chemikalien wie Amanitin

A

unterbindet RNA Synthese in Eukaryoten

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12
Q

Wobble Prinzip

A
  1. Base bei Anticodon Paarung flexibler
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13
Q

Bestandteile Initiationskomplex Translation

A

30S UE, mRNA, fMet-tRNA, IF, GTP
es fehlt 50S UE

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14
Q

Shirne Dalgarno Sequenz

A

leigt vor Startcodon auf mRNA
komplementär zum 3’-ENde der 16S rRNA
Ribisomenbindungsstelle

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15
Q

Proteinexportsysteme

A

Sec-System: transportierte Proteine ungefaltet durchs Cytoplasma
Tat-Systeme: bereits im CM gefaltet

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