Gene Expression 2

Question 1

What occurs when there are errors in gene expression

Answer 1

Can cause many uncommon diseases cystic firbrosis and can influence the predisposition of many uncommon diseases.

Question 2

What genes are mutated in breast cancer mutation

Answer 2

BRCA1 and BRCA2 Genes

Question 3

What genes are mutated in pulmonary arterial hypertension

Answer 3

Mutation in the BMPR2 gene

Question 4

What genes are mutated in cystic fibrosis

Answer 4

CFTR

Question 5

What genes are mutated in spinal muscular atrophy

Answer 5

SMN1

Question 6

What genes are mutated in the duchess muscular dystrophy

Answer 6

DMD

Question 7

How can transcription errors lead to disease

Answer 7

Over expression of the TF can cause cancer

Question 8

How can splicing cause disease

Answer 8

Alternative splicing can cause mutations found in the exon can be skipped and produce a non functional protein

Question 9

What are the steps in western blotting

Answer 9

1. Sample preparation
2. Gel Electrophoresis
3. Blotting (or transfer)
4. Blocking
5. Antibody Probing
6. Detection

Question 10

What is PCR for

Answer 10

Enables application of a specific region of DNA