Fourth Quarter Final Exam Flashcards
Embalming
The process of chemically treating the dead human body to reduce the presence and growth of microorganisms, to retard decomposition and to restore an acceptable physical appearance
Primary Purpose of Embalming
Disinfection
Secondary Purpose of Embalming
Preservation
Third Purpose of Embalming
Restoration
Proteins
- Fundamental building blocks for cells and tissues (muscle, bone, etc)
- Relatively large molecules
- Polymers for amino acids (amino acids are the fundamental building blocks for proteins)
Peptide Bonds
- The bonds that hold amino acids together in protein molecules
-A covalent chemical bond formed when a carboxyl group reacts with an amino group, releasing water
Enzymes
- Biological Catalysts: they speed up chemical reactions
- Enzymes increase the rate of decomposition
- After somatic death, enzymes do not stop their reactions
- Enzymes also contribute to decomposition
Autolysis (pertaining to enzymes)
- Auto=self, Lysis=breaking apart
- The self-digestion in cells by enzymes in the cells themselves
Amino Acids
- Molecules used by all living things to make proteins
- The building blocks of proteins
- Composed of Amines: R-NH2 and Organic Acids: R-COOH
Primary Disinfection
- Disinfection done before the embalming procedure begins
- May consist of cleaning the deceased; cleaning the eyes, mouth, or orifices
Concurrent Disinfection
- Disinfection during the embalming operation
- Disinfection that occurs during injection and cavity embalming
Terminal Disinfection
- Disinfection after the embalming operation to protect the environment
- May consist of cleaning the deceased, cleaning the instruments and the table
Five Common Characteristics of All Embalming Chemicals
- Inactivate the chemical groups of proteins or amino acids
- Inhibit further decomposition
- Inactivate enzymes (both of the tissues and microorganisms)
- Kill microorganisms
- Destroy or mask odors and eliminate their further production
Inactivate the chemical groups of proteins or amino acids
- The nitrogen in proteins are sites of decomposition
- The chemicals react with the nitrogen to change the structure of the protein (coagulation)
- Coagulation decreases the likelihood that the protein will hydrolyze
Inhibit further decomposition
- Chemicals only slow down decomposition
- therefore, embalming as soon as possible is best
Inactivate Enzymes
- Enzymes are important catalysts of hydrolysis
- Inactivating the enzymes will slow down decomposition
- enzymes are similar to proteins, so preservative chemicals will inactivate enzymes similar to tissue proteins
Kills Microorganisms
- The chemical change that proteins undergo during embalming deprives bacteria of their primary source of nutrition
- Preservative chemicals denature the structural proteins of the bacteria themselves
- The same chemical actions that cause fixation of the tissues of the remains will also produce fixation of the bacterial invaders
Destroys/Masks odors and eliminates their further production
- The odors associated with decomposition are principally due to the formation of amines
- The decomposition of structural proteins produces amines such as putrescine, cadaverine, indole, skatole
- Decomposition of sulfur-containing proteins also contributes to odors
- Protein chemicals react with the nitrogen to effectively neutralize the odor
- Preservative chemicals react with the sources of decomposition (putrefactive bacteria and autolytic enzyme) to eliminate the further production of odors
Reactions of Formaldehyde and Water
- 37% HCHO by weight
- 40% by volume
- HCHO and water produce methylene glycol
- The proteins are fixed due to the donation of a methylene group (methylene bridge)
Formaldehyde in aqueous solution is:
formalin
Reactions of Formaldehyde with Methanol
- Methanol prevents the polymerization of formaldehyde
- Helps extend shelf life