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Meharry Biochem > Final > Flashcards

Final Flashcards

1
Q

Both adenylation and uridylation play uncharacteristic roles in the formation of Gln from Glu. What are those roles?

Transfer of phosphoryl groups, forming a key reactive intermediate.

Production of additional energy from inorganic pyrophosphate acting on PPi

Regulation of Glu and Gln transport across the mitochondrial membrane

Regulation of glutamine synthetase activity

Transfer of the required second nitrogen-containing group

A

Regulation of glutamine synthetase activity

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2
Q

Which of the following statements is true about gout?

Gout is caused by excessive breakdown of pyrimidine nucleotides

Gout can be treated by administration of fluorouracil.

Gout is particularly prevalent in patients lacking xanthine oxidase

Gout leads to deposition of sodium urate crystals in the joints

Eating more liver is an effective way to treat the symptoms of gout.

A

Gout leads to deposition of sodium urate crystals in the joints

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3
Q

CMP, UMP, and TMP all have ___________ as a common precursor.

adenosine 
aspartate
glutamine
inosine
S-adenosyl methionine
A

aspartate

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4
Q

The ribosyl phosphate moiety needed for the synthesis of orotidylate, inosinate, and guanylate is provided most directly by:

5-phosphoribosyl 1-pyrophosphate
adenosine 5’-phosphate
guanosine 5’-phosphate
ribose 5-phosphate
ribulose 5-phosphate
A

5-phosphoribosyl 1-pyrophosphate

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5
Q

Orotic aciduria is an inherited metabolic disease in which orotic acid (orotate) accumulates in the tissues, blood and urine. The metabolic pathway in which the enzyme defect occurs is:

epinephrine synthesis
purine breakdown
purine synthesis
pyrimidine breakdown
pyrimidine synthesis
A

pyrimidine synthesis

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6
Q

De novo purine biosynthesis is distinguished from de novo pyrimidine biosynthesis by:

condensation of the completed purine ring with ribose phosphate

incorporation of CO2

inhibition by azaserine (a glutamine analog)

participation of aspartate

participation of PRPP (phosphoribosyl pyrophosphate)

A

incorporation of CO2

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7
Q

Which of the following is not a chemotherapeutic drug?

azaserine
allopurinol
fluorouracil
methotrexate
acivicin
A

allopurinol

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8
Q

An intermediate of purine degradation in humans is:

glutamate
NH4+ 
Succinate
Urea
Uric acid
A

Uric acid

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9
Q

A cell that is unable to synthesize or obtain tetrahydrofolic acid (H4 folate) would probably be deficient in the biosynthesis of:

CMP
GMP
Orotate
Thymidylate (TMP)
UMP
A

Thymidylate (TMP)

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10
Q

Which one of the following statements correctly describes the biosynthetic pathway for purine nucleotides?

Purine deoxynucleotides are made by the same path as ribonucleotides, followed by reduction of the ribose moiety

The first enzyme in the pathway is aspartate transcarbamoylase (ATCase)

The nitrogen in the purine base that is bonded to ribose in the nucleotide is derived originally from glycine

The pathway occurs only in plants and bacteria, not in animals

The purine rings are first synthesized, then condensed with ribose phosphate

A

Purine deoxynucleotides are made by the same path as ribonucleotides, followed by reduction of the ribose moiety

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11
Q 
The most direct precursors of the nitrogens of UMP are:
aspartate and carbamoyl phosphate
glutamate and asparate 
glutamate and carbamoyl phosphate
glutamine and aspartate
glutamine and carbamoyl phosphate
A

aspartate and carbamoyl phosphate

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12
Q

Which of the following is not true about the anammox reaction?

A. Anammox converts ammonia to nitrogen.

B. Anammox is performed by symbiotic bacteria of leguminous plants.

C. Anammox generates the highly reactive molecule hydrazine that is a component of rocket fuel.

D. Anammox occurs anaerobically.

E. The ultimate electron acceptor in anammox is nitrite.

A

B. Anammox is performed by symbiotic bacteria of leguminous plants.

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13
Q

Glutamine synthetase converts ____ to ___, whereas glutamate synthase converts _____ to ____.

A. formate; glutamine; ammonia; glutamate
B. asparagine; glutamine; alpha‐ketoglutarate; glutamate
C. alpha ketoglutarate; glutamine; oxaloacetic acid; glutamate
D. alpha‐ketoglutarate; glutamine; alpha‐ketoglutarate; glutamate
E. glutamate; glutamine; alpha‐ketoglutarate; glutamate

A

glutamate; glutamine; alpha‐ketoglutarate; glutamate

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14
Q 
An amino acid that does not derive its carbon skeleton, at least in part, from alpha‐ ketoglutarate is:
A.	arginine.
B.	glutamate.
C.	glutamine.
D.	proline.
E. 	threonine.
A

threonine

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15
Q 
If a cell were unable to synthesize or obtain tetrahydrofolic acid (H4 folate), it would probably be deficient in the biosynthesis of:
A.	isoleucine.
B.	leucine.
C.	lysine.
D. 	methionine.
E. 	serine.
A

methionine

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16
Q 
An important intermediate in the biosynthetic pathway to aromatic amino acids is:
A.	benzoic acid.
B.	lactate.
C.	orotate.
D. 	shikimate.
E.	 alpha‐ketoglutarate
A 
An important intermediate in the biosynthetic pathway to aromatic amino acids is:
A.	benzoic acid.
B.	lactate.
C.	orotate.
D. 	shikimate.
E.	 alpha‐ketoglutarate
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17
Q 
Bile pigments are:
A. 	formed in the degradation of heme.
B.	generated by oxidation of sterols.
C.	responsible for light reception in the vertebrate eye.
D.	secreted from the pancreas
E.	the products of purine degradation
A

A. formed in the degradation of heme.

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18
Q

Glutathione is a(n):
A. enzyme essential in the synthesis of glutamate.
B. isomer of oxidized glutamic acid.
C. methyl‐group donor in many biosynthetic pathways.
D. product of glutamate and methionine.
E. tripeptide of glycine, glutamate, and cysteine.

A

tripeptide of glycine, glutamate, and cysteine.

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19
Q 
Porphyrias is the disease associated with
A. Heme degradation pathway
B. Heme biosynthesis pathway
C. Tyrosine biosynthesis
D. Bromatic amino acid biosynthesis
E. Bilirubin accumulation
A

B. Heme biosynthesis pathway

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20
Q 
Which of these is not a protease that acts in the small intestine?
A.	Chymotrypsin
B.	Elastase
C.	Enteropeptidase
D. 	Secretin
E. 	Trypsin
A

D. Secretin

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21
Q

In amino acid catabolism, the first reaction for many amino acids is a(n):
A. decarboxylation requiring thiamine pyrophosphate (TPP).
B. hydroxylation requiring NADPH and O2.
C. oxidative deamination requiring NAD+.
D. reduction requiring pyridoxal phosphate (PLP).
E. transamination requiring pyridoxal phosphate (PLP).

A

transamination requiring pyridoxal phosphate (PLP).

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22
Q 
Which of these amino acids are both ketogenic and
glucogenic?
1.	Isoleucine
2.	Valine
3.	Histidine
4.	Arginine
5.	Tyrosine
A.	 1 and 5
B.	1, 3, and 5
C.	2 and 4
D.	2, 3, and 4
E.	2, 4, and 5
A

A. 1 and 5

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23
Q

Purine nucleotide and Histidine synthesis both use which pair of molecules early in the pathways?

A. 	Phosphoribosyl pyrophosphate and ATP
B.	Ribose 5‐phosphate and ATP
C.	GTP and Ribulose 5‐phosphate
D.	Pyridoxal phosphate and ATP
E.	NAD(P)H and ATP
A

A. Phosphoribosyl pyrophosphate and ATP

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24
Q

5‐Phosphoribosyl‐alpha‐pyrophosphate (PRPP) is a synthe

A

arginine

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25
Q

The synthesis of purine and pyrimidine nucleo

A

purine biosynthesis starts with the formation of PRPP, whereas pyrimidines incorporate the PRPP near the end of the pathway.

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26
Q

Precursors for the biosynthesis of the pyrimidine ring system include:
A. carbamoyl phosphate and aspartate.
B. glutamate, NH3, and CO2.
C. glycine and succinyl‐CoA.
D. glycine, glutamine, CO2, and aspartate.
E. inosine and aspartate

A

A. carbamoyl phosphate and aspartate.

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27
Q 
Orotic aciduria is an inherited metabolic disease in which orotic acid (orotate) accumulates in the tissues, blood, and urine. The metabolic pathway in which the enzyme defect occurs is:
A.	epinephrine synthesis.
B.	purine breakdown.
C.	purine synthesis.
D.	pyrimidine breakdown.
E. 	pyrimidine synthesis.
A

E. pyrimidine synthesis.

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28
Q

An enzyme used in both glycolysis and gluconeogenesis is:

A.     3‐phosphoglycerate kinase.
B.	glucose 6‐phosphatase.
C.	hexokinase.
D.	phosphofructokinase‐1.
E.	pyruvate kinase.
A

A. 3‐phosphoglycerate kinase.

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29
Q

Which one of the following statements about gluconeogenesis is false?

A. For starting materials, it can use carbon skeletons derived from certain amino acids.
B. It consists entirely of the reactions of glycolysis, operating in the reverse direction.
C. It employs the enzyme glucose 6‐phosphatase.
D. It is one of the ways that mammals maintain normal blood glucose levels between meals.
E. It requires metabolic energy (ATP or GTP).

A

It consists entirely of the reactions of glycolysis, operating in the reverse direction.

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30
Q

In humans, gluconeogenesis:

A. can result in the conversion of protein into blood glucose.
B. helps to reduce blood glucose a_er a carbohydrate-rich meal.
C. is activated by the hormone insulin
D. is essential in the conversion of fatty acids to glucose.
E. requires the enzyme hexokinase.
Which of the following substrates cannot contribute to net gluconeogenesis in the mammalian liver?

A

can result in the conversion of protein into blood glucose.

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31
Q

Which of the following substrates cannot contribute to net gluconeogenesis in mammalian liver?

A.	Alanine
B.	Glutamate
C. 	Palmitate
D. 	Pyruvate
E.	 a‐ketoglutarate
A

Palmitate

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32
Q

The coenzyme required for all transaminations is derived from:

A	niacin.
B. 	pyridoxine (vitamin B6).
C.	riboflavin.
D.	thiamin.
E.	vitamin B12
A

B. pyridoxine (vitamin B6).

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33
Q

Which of the following reactions involving an amino acid cannot be catalyzed via a PLP‐dependent mechanism?

A. 	Hydrolysis
B.	Decarboxylation
C.	Racemization
D.	Transamination
E.	Transimination
A

Hydrolysis

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34
Q

Glutamate is metabolically converted to a‐ketoglutarate and NH4+ by a process described as:

A.	deamination.
B.	hydrolysis.
C. 	oxidative deamination.
D.	reductive deamination.
E.	transamination
A

oxidative deamination.

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35
Q

The conversion of glutamate to an a‐ketoacid and NH4 :

A. does not require any cofactors.
B. is a reductive deamination.
C. is accompanied by ATP hydrolysis catalyzed by the same enzyme.
D. is catalyzed by glutamate dehydrogenase.
E. requires ATP.

A

is catalyzed by glutamate dehydrogenase.

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36
Q

Conversion of ornithine to citrulline is a step in the synthesis of:

A.	aspartate.
B.	carnitine.
C.	pyruvate.
D.	tyrosine.
E. 	urea.
A

urea

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37
Q

Which of the following amino acids are essential for humans?

A.	Alanine
B.	Aspartic acid
C.	Asparagine
D.	Serine
E. 	Threonine
A

Threonine

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38
Q

Which of these amino acids can be directly converted into a citric acid cycle intermediate by transamination?

A.	 Glutamic acid
B.	Serine
C.	Threonine
D.	Tyrosine
E.	Proline
A

A. Glutamic acid

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39
Q

Serine or cysteine may enter the citric acid cycle as acetyl‐CoA after conversion to:

A.	oxaloacetate.
B.	propionate.
C. 	pyruvate.
D.	succinate.
E.	succinyl‐CoA
A

pyruvate

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40
Q

The human genetic disease phenylketonuria (PKU) can result from:

A. deficiency of protein in the diet.
B. inability to catabolize ketone bodies.
C. inability to convert phenylalanine to tyrosine.
D. inability to synthesize phenylalanine.
E. production of enzymes containing no phenylalanine.

A

inability to convert phenylalanine to tyrosine.

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41
Q

Which of the following enzymes is not involved in the assimilation of inorganic nitrogen into an organic molecule?

A.	Dinitrogenase reductase
B.	Nitrate reductase
C.	Nitrite reductase
D. 	Nitrile reductase
E.	 Dinitrogenase
A

Nitrile reductase

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42
Q

Which of the following enzymes is not involved in the assimilation of inorganic nitrogen into an organic molecule?

A. 	Arginase
B.	Glutamate dehydrogenase
C.	Glutamate synthase
D.	Glutamine synthetase
E.	Dinitrogenase
A

Arginase

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43
Q

Glutamine, arginine, and proline:

A. do not have a common precursor.
B. may all be derived from a citric acid cycle intermediate.
C. may all be derived from a Cori cycle intermediate.
D. may all be derived from a glycolytic intermediate.
E. may all be derived from a urea cycle intermediate.

A

B. may all be derived from a citric acid cycle intermediate.

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44
Q

An amino acid that does not derive its carbon skeleton, at least in part, from oxaloacetate is:

A.	aspartate.
B.	lysine.
C.	methionine.
D.	 proline.
E. 	threonine.
A

proline

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45
Q

Erythrose 4‐phosphate is a precursor of:

A.	aspartate.
B.	cysteine.
C. 	phenylalanine.
D.	serine.
E.	threonine.
A

C. phenylalanine.

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46
Q

L‐Dopa is an intermediate in the conversion of:

A.	phenylalanine to homogentisic acid.
B.	phenylalanine to tyrosine.
C. 	tyrosine to epinephrine.
D.	tyrosine to phenylalanine.
E.	tyrosine to phenylpyruvate
A

C. tyrosine to epinephrine.

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47
Q

The amino acid that gives rise to the biological messenger NO is:

A.	 glutamine.
B. 	arginine.
C.	proline.
D.	lysine.
E.	histidine.
A

B. arginine.

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48
Q

One amino acid directly involved in the purine biosynthetic pathway is:

A. 	alanine.
B. 	aspartate.
C.	glutamate.
D.	leucine.
E.	tryptophan
A

aspartate.

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49
Q

5‐Phosphoribosyl‐a‐pyrophosphate (PRPP) is a synthetic precursor for all of the following except:

A. 	AMP.
B. 	arginine.
C.	histidine.
D.	tryptophan.
E.	UMP.
A

arginine.

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50
Q

Glutamine is a nitrogen donor in the synthesis of:

A.	CTP.
B.	dTTP.
C.	inosinic acid (IMP).
D. 	orotate.
E.	UMP.
A

C. inosinic acid (IMP).

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51
Q

All enzyme-catalyzed aminotransferase reactions remove amino groups from amino acids, producing what byproduct that is important during metabolic stress?

A.	Pyruvate
B	A hydride and a H+
C.	One of the ketone body molecules
D.	Fumarate
E.	An alpha-ketoacid
A

E. An alpha-ketoacid

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52
Q

The acidic and alkaline environments of the upper gastrointestinal tract promote what type of enzymes needed to activate pro-enzymes that are required for protein metabolism?

A.	Aminotransferases
B	Mixed-function oxidases
C.	Peptidases
D.	Dehydrogenases
E.	Hydratases
A

C. Peptidases

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53
Q

Amino acids are not stored in appreciable concentrations. However, what amino acid would be found in significant concentration in blood of basketball players after a long and intense workout in a gym?

A.	Histadine
B	Alanine
C.	Glutamine
D.	Cysteine
E.	Proline
A

Alanine

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54
Q

Two nitrogen-containing groups are used to make urea in liver cells. What two molecules of the Urea Cycle contribute to those two groups ?

A.	Carbamoyl phosphate and Asp 
B	Ala and Glu
C.	Glu and Gln
D.	Carbamoyl phosphate and Glu
E.	Arg and Asp
A

A. Carbamoyl phosphate and Asp

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55
Q

The Urea Cycle itself requires two different cellular compartments. What other pathway also requires two cellular compartments?

A.	Glycolysis
B	Pentose phosphate, oxidative phase
C.	Beta-oxidation
D.	Gluconeogenesis
E.	Glyoxylate
A

D. Gluconeogenesis

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56
Q

What molecule that is produced in the cytosol by the Urea Cycle can be used by the Citric Acid Cycle, but cannot pass the inner mitochondrial membrane?

A.	Malate
B	Arginosuccinate
C.	Ornithine
D.	NAD+
E.	Fumarate
A

Fumarate

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57
Q

Amino acid oxidation requires cofactors that can serve as a one-carbon source for amino acid and nucleotide synthesis. Which molecule below does not function as a one-carbon transfer molecule?

A.	Pyridoxal phosphate 
B	Biotin
C.	S-adenosylmethionine
D.	Tetrahydrofolate
E.	All of the above are involved in one-carbon transfers.
A

A. Pyridoxal phosphate

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58
Q

Which molecule below is produced by oxidation of most amino acids, leading to potential carbon loss by organisms that cannot fix carbon?

A.	Methane 
B	Acetate
C.	Glycine
D.	Bicarbonate
E.	Carbon dioxide
A

E. Carbon dioxide

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59
Q

What molecule is produced from oxidation of a number of amino acids, and which can be used to produce ATP efficiently via oxidative phosphorylation?

A.	NADPH 
B	Acetate
C.	NADH
D.	Ammonia
E.	FADH2
A

NADH

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60
Q

What is the metabolic use of Ketogenic amino acids?

A. All are used to produce alpha-ketoglutarate for Kreb’s Cycle.
B All are used to produce ketone bodies.
C. All are used to produce the keto-forms of carbohydrates that substitute for glycolytic intermediates.
D. All are used as one-carbon donors for the formation of alpha-ketoacids.
E. All are used in Ketogenesis, the formation of ketone-containing amino acids

A

B All are used to produce ketone bodies.

61
Q

Bacteria deep in a corn field fix nitrogen early in the spring. Once the farmer begins plowing the soil, which process would contine while the others slow or stop?

Nitrifcation
Denitrification
Fermentation
Nitrogen fixation
All of the above would slow or stop
A

Nitrifcation

62
Q

Nitrogen fixation by the Dinitrogenase Complex produces what gas?

oxygen
carbon dioxide
hydrogen
nitrogen
carbon monoxide
A

hydrogen

63
Q

Some of the pathways for amino acid and nucleotide synthesis require which molecule as an intermediate, not a cofactor or prosthetic group?

pyrixoxal phosphate
phosphoribosyl pyrophosphate
cyanocobalamine
lipoate
nicotinamide
A

pyrixoxal phosphate

64
Q

The conversion of inosinate to adenylate requires Asp, a nucleoside triphosphate, and it produces fumarate. What other pathway has this same strategy?

A. 	Cys synthesis 
B. 	Glu synthesis from Gln 
C. 	Pentose Phosphate, non-oxidative 
D.    Urea Cycle
E.    Calvin Cycle, Third Stage
A

D. Urea Cycle

65
Q

Ribonucleotide reductase was altered by site-directed mutagenesis such that Met was added in place of Cys. What effect would this have on the activity of the enzyme?

A. The active site would remove the correct hydroxyl group from ribose.

B. The active site would be non-functional.

C. The substrate specificity site would not function.

D. There would be no effect on the activity of the enzyme.

E. The enzyme would produce incorrect deoxyribonucleotides due to a lack of regulation.

A

B. The active site would be non-functional.

66
Q

Which of the following compounds cannot serve as the starting material for the synthesis of glucose via gluconeogenesis?

acetate 
glycerol 
lactate 
oxaloacetate 
a-ketoglutarate
A

acetate

67
Q

All of the following enzymes involved in the flow of carbon from glucose to lactate (glycolysis) are also involved in the reversal of this flow (gluconeogenesis) except:

3-phosphoglycerate kinase. 
aldolase. 
enolase. 
phosphofructokinase-1. 
phosphoglucoisomerase.
A

phosphofructokinase-1.

68
Q

Gluconeogenesis must use “bypass reactions” to circumvent three reactions in the glycolytic pathway that are highly exergonic and essentially irreversible. Reactions carried out by which three of the enzymes listed must be bypassed in the gluconeogenic pathway?

A- Hexokinase
B-Phosphoglycerate kinase
C-Phosphofructokinase-1
D-Pyruvate kinase
E-Triosephosphate isomerase
A 1, 2, 3
B 1, 2, 4
C 1, 4, 5
D 1, 3, 4
E 2, 3, 4
A

D

69
Q

In the digestion of protein that occurs in the small intestine, which enzyme is critical in the activation of zymogens?

Enteropeptidase 
Hexokinase 
Papain 
Pepsin 
Secretin
A

Enteropeptidase

70
Q

Which of the following is a zymogen that can be converted to an endopeptidase that hydrolyzes peptide bonds adjacent to Lys and Arg residues?

Chymotrypsinogen 
Pepsin 
Pepsinogen 
Trypsin 
Trypsinogen
A

Trypsinogen

71
Q

In amino acid catabolism, the first reaction for many amino acids is a(n):

decarboxylation requiring thiamine pyrophosphate (TPP).
hydroxylation requiring NADPH and O2.
oxidative deamination requiring NAD+.
reduction requiring pyridoxal phosphate (PLP).
transamination requiring pyridoxal phosphate (PLP).

A

transamination requiring pyridoxal phosphate (PLP).

72
Q

Transamination from alanine to α-ketoglutarate requires the coenzyme:

biotin.
NADH.
No coenzyme is involved.
pyridoxal phosphate (PLP).

A

pyridoxal phosphate (PLP).

73
Q

Pyridoxal phosphate is a cofactor in this class of reactions:

acetylation.
desulfuration.
methylation.
reduction.
transamination.

A

transamination

74
Q

Which of the following is not true of the reaction catalyzed by glutamate dehydrogenase?

It is similar to transamination in that it involves the coenzyme pyridoxal phosphate (PLP).
NH4+ is produced.
The enzyme can use either NAD+ or NADP+ as a cofactor.
The enzyme is glutamate-specific, but the reaction is involved in oxidizing other amino acids.
α-Ketoglutarate is produced from an amino acid.

A

It is similar to transamination in that it involves the coenzyme pyridoxal phosphate (PLP).

75
Q

Glutamate is metabolically converted to α-ketoglutarate and NH4+ by a process described as:

deamination.
hydrolysis.
oxidative deamination.
reductive deamination.
transamination.

A

oxidative deamination.

76
Q

The conversion of glutamate to an α-ketoacid and NH4+:

does not require any cofactors.
is a reductive deamination.
is accompanied by ATP hydrolysis catalyzed by the same enzyme.
is catalyzed by glutamate dehydrogenase.
requires ATP.

A

is catalyzed by glutamate dehydrogenase.

77
Q

Which of the following conversions require more than one step?

  1. Alanine → pyruvate
  2. Aspartate → oxaloacetate
  3. Glutamate → α−ketoglutarate
  4. Phenylalanine → hydroxyphenylpyruvate
  5. Proline → glutamate
1 and 4 
1, 2, and 4 
1, 3, and 5 
2, 4, and 5 
4 and 5
A

4 and 5

78
Q

Urea synthesis in mammals takes place primarily in tissues of the:

brain.
kidney.
liver.
skeletal muscle.
small intestine.

A

liver

79
Q

Which substance is not involved in the production of urea from NH4+ via the urea cycle?

Aspartate 
ATP
Carbamoyl phosphate 
Malate 
Ornithine
A

Malate

80
Q

Which of these directly donates a nitrogen atom for the formation of urea during the urea cycle?

Adenine 
Aspartate 
Creatine 
Glutamate 
Ornithine
A

Aspartate

81
Q

In the urea cycle, ornithine transcarbamoylase catalyzes:

cleavage of urea to ammonia.
formation of citrulline from ornithine and another reactant.
formation of ornithine from citrulline and another reactant.
formation of urea from arginine.
transamination of arginine.

A

formation of citrulline from ornithine and another reactant.

82
Q

Which of the following statements is false in reference to the mammalian synthesis of urea?

Krebs was a major contributor to the elucidation of the pathway involved.
The amino acid arginine is the immediate precursor to urea.
The carbon atom of urea is derived from mitochondrial HCO3–.
The precursor to one of the nitrogens of urea is aspartate.
The process of urea production is an energy-yielding series of reaction

A

The process of urea production is an energy-yielding series of reaction

83
Q

Which of the following amino acids are essential for humans?

alanine 
aspartic acid 
asparagine
serine
threonine
A

threonine

84
Q

If a person’s urine contains unusually high concentrations of urea, which one of the following diets has he or she probably been eating recently?

High carbohydrate, very low protein
Very high carbohydrate, no protein, no fat
Very very high fat, high carbohydrate, no protein
Very high fat, very low protein
Very low carbohydrate, very high protein

A

Very low carbohydrate, very high protein

85
Q

Which of these amino acids can be directly converted into a citric acid cycle intermediate by transamination?

glutamic acid 
serine 
threonine 
tyrosine
proline
A

glutamic acid

86
Q

Which of these amino acids are both ketogenic and glucogenic?

  1. Isoleucine
  2. Valine
  3. Histidine
  4. Arginine
  5. Tyrosine
1 and 5 
1, 3, and 5 
2 and 4 
2, 3, and 4 
2, 4, and 5
A

1 and 5

87
Q

Tetrahydrofolate (THF) and its derivatives shuttle between different substrates.

electrons 
H+ 
acyl groups 
one carbon units
NH2 groups
A

one carbon units

88
Q

The amino acids serine, alanine, and cysteine can be catabolized to yield:

fumarate.
pyruvate.
succinate.
α-ketoglutarate.
none of the above

A

pyruvate.

89
Q

Serine or cysteine may enter the citric acid cycle as acetyl-CoA after conversion to:

oxaloacetate.
propionate.
pyruvate.
succinate.
succinyl-CoA.

A

pyruvate

90
Q

The human genetic disease phenylketonuria (PKU) can result from:

deficiency of protein in the diet.
inability to catabolize ketone bodies.
inability to convert phenylalanine to tyrosine.
inability to synthesize phenylalanine.
production of enzymes containing no phenylalanine.

A

inability to convert phenylalanine to tyrosine.

91
Q

In the human genetic disease maple syrup urine disease, the metabolic defect involves:

a deficiency of the vitamin niacin.
oxidative decarboxylation.
synthesis of branched chain amino acids.
transamination of an amino acid.
uptake of branched chain amino acids into liver.

A

oxidative decarboxylation.

92
Q

ccumulates in the tissues, blood, and urine. The metabolic pathway in which the enzyme defect occurs is:

epinephrine synthesis. 
purine breakdown. 
purine synthesis. 
pyrimidine breakdown. 
pyrimidine synthesis.
A

pyrimidine synthesis.

93
Q

Precursors for the biosynthesis of the pyrimidine ring system include:

carbamoyl phosphate and aspartate. 
glutamate, NH3, and CO2. 
glycine and succinyl-CoA. 
glycine, glutamine, CO2, and aspartate. 
inosine and aspartate.
A

carbamoyl phosphate and aspartate.

94
Q

CMP, UMP, and TMP all have ________________ as a common precursor.

adenosine
aspartate
glutamine
inosine
S-adenosyl methionine
A

aspartate

95
Q

Which of the following is not true of the reaction catalyzed by ribonucleotide reductase?

Glutathione is part of the path of electron transfer.
It acts on nucleoside diphosphates.
Its mechanism involves formation of a free radical.
There is a separate enzyme for each nucleotide (ADP, CDP, GDP, UDP).
Thioredoxin acts as an essential electron carrier.

A

There is a separate enzyme for each nucleotide (ADP, CDP, GDP, UDP).

96
Q

Which one of the following statements correctly describes the biosynthetic pathway for purine nucleotides?

Purine deoxynucleotides are made by the same path as ribonucleotides, followed by reduction of the ribose moiety.

The first enzyme in the path is aspartate transcarbamoylase (ATCase).

The nitrogen in the purine base that is bonded to ribose in the nucleotide is derived originally from glycine.

The pathway occurs only in plants and bacteria, not in animals.

The purine rings are first synthesized, then condensed with ribose phosphate.

A

Purine deoxynucleotides are made by the same path as ribonucleotides, followed by reduction of the ribose moiety.

97
Q

Which of the following statements about the fixation of atmospheric nitrogen (N2) into NH3 by living cells is false?

It involves the transfer of 8 electrons per mol of N2.

It occurs in certain microorganisms, but not in humans.

It requires a source of electrons, normally ferredoxin.

It requires one ATP per mol of N2 fixed.

It requires two key protein components, each containing iron.

A

It requires one ATP per mol of N2 fixed.

98
Q

Which of the following enzymes is not involved in the assimilation of inorganic nitrogen into an organic molecule?

Arginase 
Glutamate dehydrogenase 
Glutamate synthase 
Glutamine synthetase 
Nitrogenase
A

Arginase

99
Q

The enzymatic machinery to fix atmospheric N2 into NH4+ is:

a means of producing ATP when excess N2 is available.
composed of two key proteins, each containing iron.
relatively stable when exposed to O2.
specific to plant cells.
unaffected by the supply of electrons.

A

composed of two key proteins, each containing iron.

100
Q

Erythrose 4-phosphate is a precursor of:

aspartate.
cysteine.
phenylalanine.
serine.
threonine.

A

phenylalanine.

101
Q

Nonessential amino acids:

are amino acids other than those required for protein synthesis.
are not utilized in mammalian proteins.
are synthesized by plants and bacteria, but not by humans.
can be synthesized in humans as well as in bacteria.
may be substituted with other amino acids in proteins.

A

can be synthesized in humans as well as in bacteria.

102
Q

An amino acid that does not derive its carbon skeleton, at least in part, from α-ketoglutarate is:

arginine.
glutamate.
glutamine.
proline.
threonine.

A

threonine

103
Q

Glutamine, arginine, and proline:

do not have a common precursor.

may all be derived from a citric acid cycle intermediate.

may all be derived from a Cori cycle intermediate.

may all be derived from a glycolytic intermediate.

may all be derived from a urea cycle intermediate.

A

may all be derived from a citric acid cycle intermediate.

104
Q

In which group are all the amino acids closely interrelated metabolically?

Arginine, hydroxyproline, and histidine
Arginine, tyrosine, and glutamate
Glycine, valine, glutamine, and aspartate
Ornithine, alanine, glycine, and valine

A

Ornithine, alanine, glycine, and valine

105
Q

An amino acid that does not derive its carbon skeleton, at least in part, from oxaloacetate is:

aspartate.
lysine.
methionine.
proline.
threonine.

A

threonine.

106
Q

Homoserine is:

a precursor of both methionine and threonine. 
a precursor of serine. 
derived from homocysteine. 
derived from serine. 
derived from threonine.
A

a precursor of both methionine and threonine.

107
Q

The nitrogen atom in the side chain of lysine is derived from which amino acid?

aspartic acid. 
glutamic acid. 
glutamine. 
asparagine. 
arginine
A

glutamic acid.

108
Q

δ-Aminolevulinic acid is formed from succinyl-CoA and __________ and is an intermediate in the biosynthesis of _________.

acetyl-CoA; long chain fatty acids 
glycine; heme 
serine; heme 
serine; sphingosine 
α-ketoglutarate; glutamate and proline
A

glycine; heme

109
Q

The hormones epinephrine and norepinephrine are derived biosynthetically from:

arginine.
histidine.
isoleucine.
tryptophan.
tyrosine.

A

tryptophan

110
Q

In skeletal muscle, phosphocreatine functions as:

a reservoir of Pi for mitochondria.

reservoir of high-energy of phosphate to replenish ATP.

reservoir of amino acids for protein synthesis.

an electron acceptor under anaerobic conditions.

none of the above.

A

reservoir of high-energy of phosphate to replenish ATP.

111
Q

One distinction between peptide and steroid hormones is that peptide hormones:

act through nonspecific receptors, whereas steroid hormones act through specific receptors.

are generally water-insoluble, whereas steroid hormones are water soluble.

are more stable than steroid hormones.

bind to cell surface receptors, whereas steroid hormones bind to nuclear receptors.

bind to their receptors with high affinity, whereas steroid hormones bind with low affinity.

A

bind to cell surface receptors, whereas steroid hormones bind to nuclear receptors.

112
Q

Insulin is an example of a(n) ____________ hormone.

 catecholamine 
eicosanoid 
paracrine
peptide 
steroid
A

peptide

113
Q

Epinephrine is an example of a(n) ____________ hormone.

catecholamine 
eicosanoid 
paracrine 
peptide 
steroid
A

catecholamine

114
Q

An example of an eicosanoid hormone is:

epinephrine. 
retinoic acid. 
testosterone. 
thromboxane. 
thyroxine.
A

thromboxane

115
Q

An example of a steroid hormone is:

epinephrine. 
retinoic acid. 
testosterone. 
thromboxane. 
thyroxine.
A

testosterone.

116
Q

Some hormones are derived from amino acids; for example, catecholamines are derived from while NO is derived from.

tyrosine; arginine 
tryptophan; lysine 
tyrosine; histidine 
tryptophan; arginine 
histidine; lysine
A

tyrosine; arginine

117
Q

The tropic hormones (such as thyrotropin, somatotropin, and luteinizing hormone) are produced and released by the:

anterior pituitary. 
hypothalamus. 
ovaries. 
pancreas. 
posterior pituitary.
A

anterior pituitary.

118
Q

The normal sequence of action of these components of the hormonal hierarchy is:

adrenal cortex → hypothalamus → anterior pituitary
anterior pituitary → adrenal cortex → hypothalamus
anterior pituitary → hypothalamus → adrenal cortex
hypothalamus → adrenal cortex → anterior pituitary
hypothalamus → anterior pituitary → adrenal cortex

A

hypothalamus → anterior pituitary → adrenal cortex

119
Q

In its role in the hormonal hierarchy, the hypothalamus produces and releases:

epinephrine.
insulin.
progesterone.
releasing factors.
thyroxine.

A

releasing factors.

120
Q

When blood glucose is abnormally high, the pancreas releases:

epinephrine.
glucagon.
glucose.
insulin.
trypsin.

A

insulin

121
Q

When blood glucose is abnormally low, the pancreas releases:

epinephrine.
glucagon.
glucose.
insulin.
trypsin

A

glucagon.

122
Q

An elevated insulin level in the blood:

inhibits glucose uptake by the liver.

inhibits glycogen synthesis in the liver and muscle.

results from a below-normal blood glucose level.

stimulates glycogen breakdown in liver.

stimulates synthesis of fatty acids and triacylglycerols in the liver.

A

stimulates synthesis of fatty acids and triacylglycerols in the liver.

123
Q

An elevated insulin level in the blood:

inhibits glucose uptake by the liver.

inhibits glycogen synthesis in the liver and muscle.

results from a below-normal blood glucose level.

stimulates glycogen breakdown in liver.

stimulates synthesis of fatty acids and triacylglycerols in the liver.

A

stimulates synthesis of fatty acids and triacylglycerols in the liver.

124
Q

The radioimmunossay (RIA) is based on competition of unlabeled and radiolabeled:

antibodies for binding to a hormone.

antibodies for binding to a receptor.

hormone for binding to a receptor.

hormone for binding to an antibody.

receptor for binding to a hormone.

A

hormone for binding to an antibody.

125
Q

One distinction between peptide and steroid hormones is that peptide hormones:

act through nonspecific receptors, whereas steroid hormones act through specific receptors.

are generally water-insoluble, whereas steroid hormones are water soluble.

are more stable than steroid hormones.
bind to cell surface receptors, whereas steroid hormones bind to nuclear receptors.

bind to their receptors with high affinity, whereas steroid hormones bind with low affinity.

A

are more stable than steroid hormones.

bind to cell surface receptors, whereas steroid hormones bind to nuclear receptors.

126
Q

Insulin is an example of a(n) ____________ hormone.

catecholamine 
eicosanoid 
paracrine
peptide 
steroid
A

peptide

127
Q

The maturation of insulin from its precursor (preproinsulin) involves:

acetylation.
oxidation.
phosphorylation.
proteolysis.
reduction.

A

proteolysis.

128
Q

Epinephrine is an example of a(n) ____________ hormone.

catecholamine 
eicosanoid 
paracrine 
peptide 
steroid
A

catecholamine

129
Q

An example of an eicosanoid hormone is:

epinephrine. 
retinoic acid. 
testosterone. 
thromboxane. 
thyroxine.
A

thromboxane.

130
Q

An example of a steroid hormone is:

epinephrine. 
retinoic acid. 
testosterone. 
thromboxane. 
thyroxine.
A

testosterone.

131
Q

Some hormones are derived from amino acids; for example, catecholamines are derived from while NO is derived from .

tyrosine; arginine 
tryptophan; lysine 
tyrosine; histidine 
tryptophan; arginine 
histidine; lysine
A

tryptophan; arginine

132
Q

The tropic hormones (such as thyrotropin, somatotropin, and luteinizing hormone) are produced and released by the:

anterior pituitary. 
hypothalamus. 
ovaries. 
pancreas. 
posterior pituitary.
A

anterior pituitary.

133
Q

The normal sequence of action of these components of the hormonal hierarchy is:

adrenal cortex → hypothalamus → anterior pituitary

anterior pituitary → adrenal cortex → hypothalamus

anterior pituitary
→ hypothalamus → adrenal cortex

hypothalamus → adrenal cortex → anterior pituitary

hypothalamus → anterior pituitary → adrenal cortex

A

hypothalamus → anterior pituitary → adrenal cortex

134
Q

In its role in the hormonal hierarchy, the hypothalamus produces and releases:

epinephrine.
insulin.
progesterone.
releasing factors.
thyroxine.

A

releasing factors.

135
Q

Which of the following statements about metabolism in the mammalian liver is false?

Most plasma lipoproteins are synthesized in the liver.

The enzymatic complement of liver tissue changes in response to changes in the diet.

The liver synthesizes most of the urea produced in the body.

The presence of glucose 6-phosphatase makes liver uniquely able to release glucose into the bloodstream.

Under certain conditions, most of the functions of the liver can be performed by other organs.

A

Under certain conditions, most of the functions of the liver can be performed by other organs.

136
Q

Glucokinase:

acts in the conversion of liver glycogen to glucose 1-phosphate.

converts fructose-6-phosphate to glucose-6-phosphate

converts glucose 6-phosphate to fructose 6-phosphate.

is a hexokinase isozyme found in liver hepatocytes.

is found in all mammalian tissues

A

is a hexokinase isozyme found in liver hepatocytes

137
Q

In skeletal muscle:

amino acids are an essential fuel.

at rest, fatty acids are the preferred fuel.

large quantities of triacylglycerol are stored as fuel.

phosphocreatine can substitute for ATP as the direct source of energy for muscle contraction.

stored muscle glycogen can be converted to glucose and released to replenish blood glucose.

A

at rest, fatty acids are the preferred fuel.

138
Q

In skeletal muscle, phosphocreatine functions as:

a reservoir of Pi for mitochondria.

reservoir of high-energy of phosphate to replenish ATP.

reservoir of amino acids for protein synthesis.

an electron acceptor under anaerobic conditions.

none of the above.

A

reservoir of high-energy of phosphate to replenish ATP.

139
Q

The Cori cycle is:

the conversion of lactate to pyruvate in skeletal muscle to drive glycogen synthesis.

the interconversion between glycogen and glucose l-phosphate.

the production of lactate from glucose in peripheral tissues with the resynthesis of glucose from lactate in liver.

the synthesis of alanine from pyruvate in skeletal muscle and the synthesis of pyruvate from alanine in liver.

the synthesis of urea in liver and degradation of urea to carbon dioxide and ammonia by bacteria in the gut.

A

he production of lactate from glucose in peripheral tissues with the resynthesis of glucose from lactate in liver.

140
Q

Which one of the following statements is true?

The brain prefers glucose as an energy source, but can use ketone bodies.

Muscle cannot use fatty acids as an energy source.

In a well-fed human, about equal amounts of energy are stored as glycogen and as triacylglycerol.

Fatty acids cannot be used as an energy source in humans because humans lack the enzymes of the glyoxylate cycle.

Amino acids are a preferable energy source over fatty acids.

A

The brain prefers glucose as an energy source, but can use ketone bodies.

141
Q

When blood glucose is abnormally high, the pancreas releases:

epinephrine.
glucagon.
glucose.
insulin.
trypsin.

A

insulin

142
Q

The largest energy store in a well-nourished human is:

ATP in all tissues.

blood glucose.

liver glycogen.

muscle glycogen.

triacylglycerols in adipose tissue.

A

triacylglycerols in adipose tissue.

143
Q

levated epinephrine levels do not normally stimulate:

fatty acid mobilization in adipose tissue. 
gluconeogenesis in liver. 
glycogen breakdown in muscle. 
glycogen synthesis in liver. 
glycolysis in muscle.
A

glycogen synthesis in liver.

144
Q

Epinephrine triggers an increased rate of glycolysis in muscle by:

activation of hexokinase.

activation of phosphofructokinase-1.
conversion of glycogen
phosphorylase a to glycogen
phosphorylase b.

inhibition of the Cori Cycle

the Pasteur effect.

A

activation of phosphofructokinase-1.

145
Q

Long-term maintenance of body weight is regulated by the hormone:

adiposin.
hypothalmin.
leptin.
obesin.
testosterone

A

leptin

146
Q

Among its numerous metabolic effects, the protein leptin:

decreases the production of glucocorticoids.

inactivates the enzyme 5’-AMP-activated protein kinase (AMPK).

increases the production of sex hormones.

makes muscle and liver cells more sensitive to insulin.

raises the production of thyroid hormone.

A

makes muscle and liver cells more sensitive to insulin.

147
Q

The hormone leptin appetite; insulin appetite.

increases; increases

increases; decreases

decreases; increases

decreases; decreases

no effect; no effect

A

decreases; decreases

148
Q

The peptide hormone adiponectin, produced in adipose tissue, circulates in the blood and:

enhances fatty acid synthesis in liver cells.

increases the rate of β-oxidation of fatty acids in muscle cells.

inhibits glucose uptake and catabolism in muscle and liver cells.

reduces the transport of fatty acids into muscle cells.

stimulates gluconeogenesis in liver cells.

A

increases the rate of β-oxidation of fatty acids in muscle cells.

Meharry Biochem (17 decks)

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