Ezyme Test

Question 1

Testing for sugars

Answer 1

1. Add 5cm3 of each food solution into each test tube making a note of which solution is in which tube
2. Add an equal volume of Benedict’s solution to each test tube.
3. Fill a beaker with hot water from a kettle
4. Add test tubes to the beaker. Wait for a positive result = Yellow

Question 2

Testing for Starch

Answer 2

1. Add 5cm3 of each food solution into each test tube making a note of which solution is in which test tube
2. Add a few drops of iodine solution to each test tube.
3. Observe and note the solution with a positive result = black

Question 3

Testing for protein

Answer 3

1. Add 5cm3 of each food solution into each test tube making a note of which solution is in which test tube
2. Add an equal volume of biuret solution to each test tube.
3. Observe and note the solution with a positive result = purple

Question 4

Testing for fats

Answer 4

1. Add 5cm3 of each food solution into the each test tube making a note of which solution is in which test tube
2. Add 2cm3 of ethanol solution to each test tube and shake vigorously
3. After shaking add 2cm3 of water to each test tube. Observe positive result = white cloudiness

Question 5

What colour is iodine test

Answer 5

Positive - black
Iodine - brown/yellow

Question 6

What colour is sugars

Answer 6

Positive - yellow
Sugar - blue (Benedict’s)

Question 7

What colour is protein

Answer 7

Positive - purple
Biuret - blue

Question 8

What colour is fats

Answer 8

Positive - white cloudy emulsion
Fats - colourless (ethanol)

Question 9

Enzyme for starch, sugars, protein, fats

Answer 9

Iodine
Benedict’s
Biuret
Ethanol