Exam 4 Notes Flashcards
Chemically, when embalming solution contacts protein, the long slender protein molecules are chemically bound to the neighboring protein molecules. This is called
Inter-protein crosslinking
The formation of new chemical bonds between previously unattached proteins causes tissues to do what?
Become firm
Firmness is accomplished by taking soluble albumins and altering them to become what?
Insoluble Albuminoids
This is the ideal environment for bacterial growth and reproduction
Soluble Albumins
These create an environment that is not desirable for bacterial growth and reproduction
Insoluble Albuminoids
Is it possible for bacteria to grow and reproduce in an insoluble albuminoid environment?
Yes it is possible, just undesirable
Eventually, what will happen to the insoluble albuminoids?
They will revert to albumins
Dead remains often have a foul odor associated with them. What causes this?
Several different organic compounds
What does formaldehyde do to most of the organic compounds?
Inactivated
List the 5 Complex amines aka Organic Polyamines that are the primary class of compounds associated with odor
Indole Skatole Putrescine Cadaverine Neurine
When bacteria are present, bacterial enzymes also attack the proteins of the body, hydrolyzing them into these
Peptides and amino acids
Aerobic bacteria utilize oxygen in the process of breaking down substances, converting proteins into these
Organic Acids
Carbon Dioxide
Ammonia
Anaerobic bacteria do not require oxygen. The end products of protein degradation by anaerobic organisms are
Carbon Dioxide
Hydrogen
Organic Polyamines
Both aerobic and anaerobic bacteria will produce this when interacting with proteins
An Odor
Enzymes are a specialized classification of what?
Proteins
An organic catalyst which alters the rate of a biochemical reaction, but is not consumed in the process
Enzyme
A few molecules of an enzyme can convert a large amount of reactants into what?
Products
Virtually every substance in the body may be metabolized by enzymes of various specificities into what?
More Simple Compounds
The enzyme arginase breaks down the amino acid
Arginine
The enzymes lipase breaks down
Lipids
The enzyme sucrase breaks down
Sucrose
Enzymes are sensitive to changes in
pH or temperature
Enzymes are vulnerable to inactivation by
Formaldehyde
Failure to inhibit the activity of certain enzymes would result in what?
Decomposition of the entire body
A postmortem stiffening of the muscles
Rigor Mortis
Where does rigor start?
In the jaw and works it’s way downward
Following death, glycogen is oxidized to
Sarcolactic acid
Sarcolactic acid may cause the plasma pH to drop as low as
5.3
The acidification normally observed is between
6-5.5
The drop in pH causes the soluble muscle proteins, myosin and myosinogen, to coagulate and form what?
Myosin-fibrin and Myosinogen-fibrin
Coagulation of protein causes the muscles to ____
shrink
The shrinking of muscles causes what?
Rigor mortis
Rigor Mortis disappears ______ later
1-6 days
There are two factors that contribute to the release of rigor. What are they?
Acid neutralization by ammonia and the breakdown of coagulated proteins by hydrolytic enzymes
This process is of great importance to embalming chemistry
Autolysis
Enzymes that accelerate this are found in all living matter
Hydrolysis
During life, the body is always in a constant balance of building tissue and breaking down tissue. This is known as
Necrobiosis
After death, no new tissue is being formed while enzymes that do this are still active
Degrade
The result of no new formation of new tissue while enzymes degrading still active in a dead body is self-degradation termed ________
Autolysis
This self-destructive process of autolysis breaks down proteins into
Peptides and amino acids
When formaldehyde gas reacts with tissue protein, what is formed?
A gel or resin
The protoplasm of the tissue cells is what?
A Glue-Like Colloid
The protoplasm of the tissue cells have large molecules that have the ability to arrange themselves into
Chains and meshes
The meshes and chains formed by the protoplasm of the tissue cells enclose water molecules to create the appearance of what?
A solid substance
This occurs when HCHO gas, carried to the tissue cell protoplasm by water, is liberated
Firmness or Tissue Fixation
This is a liquid at room temperature, significantly less dehydrating, and contains many more times disinfectant qualities than formaldehyde
Gluteraldehyde
If gluteraldehyde has so many more benefits than formaldehyde, then why do we still use formaldehyde?
HCHO is safer and creates the firmness that embalmers are used to
Every protein is composed of a variable number of amino acids, usually ____ hooked together by peptide bonds
20
How many total amino acids are there?
26
Amino acids in a protein molecule are rather loosely hooked together, and as this sets in, these acids easily breakdown
Decomposition
Decomposition time from death to embalming is called what?
Postmortem Interval
The sooner the remains are embalmed, the lesser will be the breakdown of protein, and the lesser this will be
HCHO demand
These are the most important and destructive of the alkalies
Simple and complex amines formed as the end products of putrefaction
The simple and complex amines formed as the end products of putrefaction have a tendency to combine with water to form hydroxide, which is a stronger base than
Ammonia
Decomposing bodies during injection seldom firm and always do what?
Swell
A recently deceased remains will firm up immediately and will
Dehydrate and shrivel
Ammonia does what to formaldehyde
Neutralizes
An acid is a substance which ________ hydrogen ions when in solution
Liberates
Solution strength is determined by this
The number of ions liberated of either acid or base
Neutralization is the reaction between what?
An acid and a base
The result of neutralization is what?
Salt and water
Alkaline salts are formed by the reaction between what?
A strong base and a weak acid
The normal pH of live body tissues and fluids is
7.4
The pH of body tissues and fluids just after death is
6.5
The pH of body tissues and fluids just after death can get as low as
5.0
Putrefaction is likely to occur at a pH of
8.0
Arterial fluids should have a pH of
7.3 (Slightly alkaline)
What do salts function as in embalming chemistry?
Water conditioners Anti-Coagulants Buffers Tension Reducers Preservatives
These are two of the many chemicals employed in present day embalming fluids
Formalin and alcohol
Examples of compounds called salts used in embalming chemicals are
Potassium Acetate
Sodium Nitrate
Aluminum Salts
Salts influence this produced by the preservative solution
Chemical Reactions
Fluid strength in terms of HCHO
Index
Primary dilution that is arterial fluid and water in the machine
Solution Volume
Amount of arterial fluid solution lost from the vascular system
Drainage Loss
Amount of HCHO liberated by way of the vascular system into the tissue
HCHO residual
The amount that remains after injection has stopped in excess of minimum requirements
HCHO residual
Commercial embalming fluids are composed of various chemicals, but the same embalming chemicals, when used in different concentrations, may produce
Different Effects
List the 9 qualities or properties that chemical solutions should display in AFS
Color Odor Viscosity Clearness Density Hardening Power pH Penetrability/Diffusibility
In order for a chemical preservative solution to do its work well, it must be thoroughly what?
Dissolved
The substance that is dissolved in a solution
Solute
Liquid containing dissolved substance
Solution
A liquid holding another substance in solution
Solvent
When the solute and the solvent are completely mixed to be one product
True Solution
HCHO as a germicide causes this
Coagulation and dehydration of the bacterial cell protoplasm
The degree of preservation results from the conversion of the soluble albumin into an insoluble albuminoid, which is more highly resistant to what?
Bacterial Action
The organs contained in the thoracic and abdominal cavities must receive supplementary treatment. Fluids formulated for this are called what?
Cavity Fluids
How many basic differences are there between cavity fluid and arterial fluids in terms of composition
6
Cavity fluids contain a much higher concentration of these
Disinfectants and preservative
Cavity fluids generally do not contain what?
Modifying Agents
Active Dyes
Deodorants
Cavity fluids often contain this bleaching agent
Phenol