Exam 3: Pentose Phosphate Pathway

Question 1

Location

Answer 1

Cytosol

Question 2

No ATP is __ or __

Answer 2

Produced or consumed

Question 3

First step (glucose 6P -> 6 phosphoglucono-o-lactone)

Answer 3

Glucose 6 DH

Question 4

Anything that consumes ___ and __ stimulates PPP

Answer 4

NADPH

Ribose

Question 5

Oxidative phase are ___ steps

Answer 5

Irreversible

Question 6

Oxidative Phase makes

Answer 6

2 NADPH

Question 7

NADPH used in

Answer 7

Reductive biosynthesis
Detoxification
Respiratory burst
Antioxidant regeneration

Question 8

Non -oxidative phase are __ steps

Answer 8

Reversible

Question 9

Ribose 5 phosphate can be used for

Answer 9

Nucleic acids
ATP
CoA
NAD, FAD

Question 10

Ribose 5 phosphate can be turned into ___ and ___ for glycolysis

Answer 10

Glyceraldehyde 3P

Fructose 6P

Question 11

Using an epimerase, ribose 5 phosphate produces

Answer 11

Xylulose 5 phosphate (X5P)

Question 12

Increase in sugar causes G6P to enter glycolysis and PPP, which increases

Answer 12

X5P

Question 13

X5P binds ____, which dephosphorylates PFK2

Answer 13

Phosphatase 2A

Question 14

PFK2 activates PFK1 and inhibits FBPase1, blocking

Answer 14

Gluconeogenesis

Question 15

These 2 enzymes help transition between ribose 5P and glycolysis intermediates

Answer 15

Transketolase

Transaldolase

Question 16

NADPH can be used to build

Answer 16

Fat
Cholesterol
Steroid hormones
Bile salts

Question 17

Detoxification makes toxins ___ by adding ___ so they can be excreted in urine

Answer 17

Water soluble

-OH

Question 18

Detoxification occurs where?

Answer 18

ER of liver

Question 19

___ system adds -OH to aromatic and aliphatic compounds for detoxification

Answer 19

Cytochrome p450 monooxygenase system

Question 20

Respiratory burst means WBC are taking in more

Answer 20

O2

Question 21

O2 + electrons =

Answer 21

Reactive molecules

Question 22

Goal of respiratory burst

Answer 22

Create harsh chemistry to kill bacteria

Question 23

Superoxide

Answer 23

O2-

Question 24

O2- —> H2O2

Answer 24

Superoxide dismutase

Question 25

H2O2

Answer 25

Hydrogen peroxide

Question 26

H2O2 —> HOCl

Answer 26

Myeloperoxidase

Question 27

HOCl

Answer 27

Hypochlorous Acid

Question 28

Antioxidant regeneration goal

Answer 28

Add election to antioxidants to keep protecting body from free radicals “Quenching” free radicals

Question 29

ROS or RNS attack protein in ___, ___ and ___

Answer 29

Cells DNA Phospholipids

Question 30

O2- created by

Answer 30

ETC Cytochrome p450 WBC

Question 31

Superoxide dismutase found

Answer 31

Everywhere Cytoplasm Mitochondria ECM

Question 32

H202 —> H2O + O2

Answer 32

Catalase

Question 33

Catalase found

Answer 33

Cell peroxisomes

Question 34

H2O2 —> 2H2O

Answer 34

Glutathione peroxidase

Question 35

Glutathione peroxidase found

Answer 35

Cytosol | Mitochondria