Exam 2 Lecture 18 Flashcards
Describe the Gram stain of Neisseria
Gram negative diplococci
True or False: Neisseria is non-motile
T
There are commensals such as N. lactamica, and this is present in the _________. It plays a large role in ______ and antibiotic _________.
oropharynx
immunity
resistance
How can Neisseria be grown in culture?
It needs to be enriched and contain CO2
What sugars do gonococcus utilize? What about meningococcus?
Gonococcus- Glucose
Meninges- maltose and glucose
True or False: Neisseria can be introduced into animals and studied for pathogenesis
False
Name the major virulence factors
Pili
OMPs (Opa, Por)
Lipooligosaccharide (LOS)
Pili attach to ____ on male urogenital epithelial cells, and on ___ on female cervical epithelium
CD46
CR3
How do pili undergo phase variation?
Transcriptional regulation
How do pili undergo antigenic variation?
Diff sequences
Genes in __ can be combined into an expressed site, known as ____
pilS, pilE
Recombination is either ________ or via _________
intrachromosomal
transformation
How many silent gene copies does the pilus have?
17
How many variable regions does the pilus have?
6
____ is the most abundant structural surface protein in Neisseria
Por
___ is involved in attachment and invasion of host cells by binding to CR3 receptors in women
Por
While there are 12 genes for Opa in _ ______, there are about 3-4 in __ __________
N. gonorrhoeae
N. meningitidis
A single strain may express ___ Opa proteins
0-3
When Opa is expressed, colonies are ______. When Opa is not expressed, colonies are _______.
opaque
transparent
