Embryology Flashcards
4 stages of embryonic development in ALL animals
1) Fertilization
2) Cleavage
3) Gastrulation
4) Organogenesis
Homologues of this drosophila gene are important in embryonic development
Tinman gene - regulates heart position in drosophila
3 key events in fertilization
1) Contact
2) Acrosomal reaction
3) Cortical reaction
Contact
1st stage of fertilization
- Sperm makes contact with protective egg coating (Zona pelludica)
Acrosomal reaction
2nd stage of fertilization
- enzymes dissolve jelly coat
- molecules on sperm bind to receptors on egg
- species specific binding process (not in all mammals)
Cortical reaction
Changes at surface of egg prevent further sperm from entering - Polyspermy
Zona Pellucida
Outer jelly coating of the females egg while still in the ovary
What occurs when the head of the sperm contacts an egg
Spermatozoon touches Zona pellucida
- triggers acrosomal process
- spermatozoon release hydrolytic enzymes
from the acrosome (head)
What is the significant process within the acrosomal process within fertilization?
Release of actin filaments
- pierce the jelly coat
- Proteins on the tip of the acrosomal process bind to receptors and act as lock and key
Acrosomal process - Non-Mammals
Quick-Block Process
- Binding of proteins on acrosomal process causes fusion of spermatozoon and egg
- Fusion triggers Na release
- Na influx causes depolarization
- Depolarization blocks other sperm from fusing
Cortical Reaction
All Mammals Longer lasting - block polyspermy - Cortical granules beneath egg membrane released into perivitelline space - Hardens - Receptors clipped off - No further binding of other sperm
What is is called when rapid cell division occurs and a single egg (zygote) forms many smaller cells?
Blastomeres
What are the first 5-7 divisions called?
Blastula
Blastocyst (in mammals)
What is it called when many small cells surround a fluid filled space?
Blastocoel
What are the two main types of cleavage systems?
1) Holoblastic
2) Meroblastic
Holoblastic cleavage
- yolk concentrated at vegetal pole
- indentation causes “cleavage furrow”
- 1st cleavage: furrow crosses over both vegetal and animal pole
- 2nd cleavage: 90deg. to 1st cleavage
- 3rd cleavage: produces 8 distinct cells