dna replication (f) Flashcards
what is dna
double stranded, antiparallel, a double helix, and is made of nucleotides
double stranded
complementary strands
antiparallel
runs in opposite directions
double helix
two backbones to the helix
made of nucleotides
adenine thymine guanine cytosine
what are the parts of a nucleotide
nitrogen base, phosphate, and sugar
sugar also known as
deoxyribose
nitrogen base
makes nucleotides different from one anoter
what are the enzymes involved in dna repliaction
primase, polymerase, ligase, helicase
primase
starts dna synthesis
dna polymerase
adds dna bases and proofreads the strand
dna ligase
seals gaps in dna by connecting dna segments
helicase
opens the two strands of dna, creates the origin of replication
leading strand
continous replication
lagging strand
discontinous replication
leading strand
made towards the replication fork
how many primers do you need for leading strand
1
continuous or discontinuous replication in leading strand?
continuous
lagging strand
made away from the replication fork
how many primers are needed for lagging strand
many
continuous or discontinuous replication in lagging strand?
discontinuous, creates okazaki fragments
how many steps are in dna replication
4
first step of dna replication
Helicase opens the helix, creating the origin of replication
second step of dna replication
Primase puts down a primer to tell Polymerase where to begin
third step of dna replication
Polymerase puts down new base pairs, building the complementary strand
fourth step of dna replication
Ligase binds all of the fragments of DNA together, to create a continuous strand
nondisjunction
homologous chromosomes that failed to separate in meiosis
what is the result in nondisjunction
trisomy or monosomy
what is trisomy
three extra chromosomes
what is monosomy
one extra chromosomes
xy
male
xx
female
karyotype
a visual to show a complete set of chromosomes
when does dna replicate
right before it divides
semi-conservative
two copies contain one old original strand and one new strand
5’ -> 3’
dna polymerase always builds in this direction
antiparallel (5’ -> 3’)
dna strands run in opposite direction, one goes in the 5 -> 3 direction while the other goes in the 3’ -> 5’ direction
prime
the carbons on the nitrogen base are numbered
the apostrophes are called prime
what direction are leading and lagging strand in
5’ -> 3’’