Diagnosis Flashcards
• Bacterial culture — GOLD STANDARD
• ELISA
• Skin test
• Rose bengal test (pink granulation)
Brucellosis
• Gram positive
• Bacterial culture for hemolysis
• Catalase test - negative
• Multiplex PCR
Streptococcosis
• Dark Field microscopy
• Thiosulfate-citrate-bile-salts sucrose (selective) blood & stool sample
• PCR
• Rapid test
Vibriosis
Vibrio that CAN ferment sucrose
Vibriocholerae
Vibrio that CANNOT ferment sucrose
Vibrio parahaemolyticus
• Ornithine decarboxylase test
- Bromocresol purple
- Cresol red
- Dextrose fermentation
- Putrescine production
• Bacterial culture
- MacConkey agar
- Xylose desoxychocolate agar
- Desoxychocolate citrate agar
• ELISA (S. dystenteriae)
• Rapid test
Shigellosis
caused by Shigella SONNEI
myocarditis
caused by Shigella FLEXNERI
reactive arthritis
(2) with Hemolytic Uremic Syndome
- Shigellosis
- Collibacillosis
• Brilliant Green agar
- placenta
- feces
- fetal tissue
- blood
- eggs
- bone marrow
• PCR
• ELISA
• Agglutination test*
Salmonellosis
most reliable: Pulsed field gel electrophoresis
• Trypticase soy broth
• Sorbitol-MacConkey agar + Phage typing
• Restriction fragment length POLYmorphism
• PCR
• Rapid tests
- Immunological
- Nucleic acid-based
- detect O & H antigens
Collibacillosis
bacterial culture for Y. enterolytica
Cefsulodin-irgasan-novobiocin
bacterial culture for Y. pseudotuberculosis
MacConkey
ELISA may have false-postive due to cross-contamination of other bacteria
Yersiniosis
immunoblot assay
Yersiniosis
• Bipolar staining
- Wright giemsa
- Wayson stain
• Bacterial culture (CIN, blood, MacConkey, nutrient agar)
• Immunofluorescence
• Latex agglutination test
• PCR
• Rapid test
Plague
• Bacterial culture
- synovial fluid
- TSI agar test
- blood
• PCR
Erysipeloid
• Chocolate
• Dextrose starch
• NOT in MacConkey
• Impression smear
• LAMP
• Disk diffusion
• Agglutination
• PCR
• ELISA
Pasteurellosis
• modified Middlebrook 7H10 / 7H11 agar
• Stonebrink / Lowerstein-Jensen egg-based medium
• biopsy (pooled lymph nodes)
• Histo
• Ziehl-Neelsen stain
• Spoligotyping
• Whole genome sequencing
Tuberculosis
primary screening test for cattle
• injection of bovine tuberculin purified protein derivative
tuberculin skin test
false-positive of Tuberculin skin test
M. avium
false-negative of Tuberculin skin test
acute & late chronic phase,
immunosuppression
3 types of Tuberculin skin test
- Caudal fold test
- Cervical intradermal test (better than CFT)
- Comparative cervical test
substitute or complement skin test
Gamma-interferon release assays
• need sample from environment
• mouse neutralization test
• ethanol treatment prior bacterial culture
Botulism
