Diagnosis

Question 1

• Bacterial culture — GOLD STANDARD
• ELISA
• Skin test
• Rose bengal test (pink granulation)

Answer 1

Brucellosis

Question 2

• Gram positive
• Bacterial culture for hemolysis
• Catalase test - negative
• Multiplex PCR

Answer 2

Streptococcosis

Question 3

• Dark Field microscopy

• Thiosulfate-citrate-bile-salts sucrose (selective) blood & stool sample

• PCR
• Rapid test

Answer 3

Vibriosis

Question 4

Vibrio that CAN ferment sucrose

Answer 4

Vibriocholerae

Question 5

Vibrio that CANNOT ferment sucrose

Answer 5

Vibrio parahaemolyticus

Question 6

• Ornithine decarboxylase test
- Bromocresol purple
- Cresol red
- Dextrose fermentation
- Putrescine production

• Bacterial culture
- MacConkey agar
- Xylose desoxychocolate agar
- Desoxychocolate citrate agar

• ELISA (S. dystenteriae)
• Rapid test

Answer 6

Shigellosis

Question 7

caused by Shigella SONNEI

Answer 7

myocarditis

Question 8

caused by Shigella FLEXNERI

Answer 8

reactive arthritis

Question 9

(2) with Hemolytic Uremic Syndome

Answer 9

1. Shigellosis
2. Collibacillosis

Question 10

• Brilliant Green agar
- placenta
- feces
- fetal tissue
- blood
- eggs
- bone marrow

• PCR
• ELISA
• Agglutination test*

Answer 10

Salmonellosis

Question 11

most reliable: Pulsed field gel electrophoresis

• Trypticase soy broth
• Sorbitol-MacConkey agar + Phage typing
• Restriction fragment length POLYmorphism

• PCR
• Rapid tests
- Immunological
- Nucleic acid-based
- detect O & H antigens

Answer 11

Collibacillosis

Question 12

bacterial culture for Y. enterolytica

Answer 12

Cefsulodin-irgasan-novobiocin

Question 13

bacterial culture for Y. pseudotuberculosis

Answer 13

MacConkey

Question 14

ELISA may have false-postive due to cross-contamination of other bacteria

Answer 14

Yersiniosis

Question 15

immunoblot assay

Answer 15

Yersiniosis

Question 16

• Bipolar staining
- Wright giemsa
- Wayson stain

• Bacterial culture (CIN, blood, MacConkey, nutrient agar)

• Immunofluorescence
• Latex agglutination test

• PCR
• Rapid test

Answer 16

Plague

Question 17

• Bacterial culture
- synovial fluid
- TSI agar test
- blood

• PCR

Answer 17

Erysipeloid

Question 18

• Chocolate
• Dextrose starch

• NOT in MacConkey

• Impression smear

• LAMP
• Disk diffusion
• Agglutination

• PCR
• ELISA

Answer 18

Pasteurellosis

Question 19

• modified Middlebrook 7H10 / 7H11 agar
• Stonebrink / Lowerstein-Jensen egg-based medium
• biopsy (pooled lymph nodes)

• Histo
• Ziehl-Neelsen stain
• Spoligotyping
• Whole genome sequencing

Answer 19

Tuberculosis

Question 20

primary screening test for cattle
• injection of bovine tuberculin purified protein derivative

Answer 20

tuberculin skin test

Question 21

false-positive of Tuberculin skin test

Answer 21

M. avium

Question 22

false-negative of Tuberculin skin test

Answer 22

acute & late chronic phase,
immunosuppression

Question 23

3 types of Tuberculin skin test

Answer 23

1. Caudal fold test
2. Cervical intradermal test (better than CFT)
3. Comparative cervical test

Question 24

substitute or complement skin test

Answer 24

Gamma-interferon release assays

Question 25

• need sample from environment
• mouse neutralization test
• ethanol treatment prior bacterial culture

Answer 25

Botulism