D2.1: Cell and Nuclear Division Flashcards

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1
Q

What is cell division

A
  • one cell into two cells (mitosis and meiosis)
  • TERMS: once cell (mother cell) → two cells (daughter cells)
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2
Q

The implications of cell division

A
  • Cells come from Pre-existing cells (Cell Theory)
    • Implications: every cell could be traced to the first cells of our being — the zygote (sperm + egg)
    • A continuity of life — going back to our origins to the present.
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3
Q

Do all living things reproduce?

A

They all can reproduce but some might have problems (e.g. infertile humans)
- has the capacity but, depending on the organism themselves, may not have the ability to reproduce

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4
Q

How does bacteria reproduce when they have no nucleus?

A
  • Binary fission
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5
Q

Asexual reproduction

A

one parent only, genetically identical to the parent (e.g. binary fission)

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6
Q

Some examples of asexual reproduction

A
  • e.g. flatworms can undergo binary fission
    • When you cut them, they can produce two offspring
  • e.g. hydra
    • they use budding, making a mini version, cut off small part → offspring.
  • e.g. starfish
    • fragmentation, cut off the arm of a starfish, offspring will form from the arm
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7
Q

How do bacteria create genetic variance

A

bacterias conjugate with each other

Before binary fission

“conjugation” they transfer DNA to each other to create genetic variance, 1 giver and 1 recipient

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8
Q

Sexual reproduction

A

2 parents -> genetically unique offspring, increases genetic variation within species -> ^ chance of favourable traits for survival
- Meiosis form of cell division, cell divides, → 4 daughter cells.

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9
Q

How do we grow?

A

By increasing our cells (like lego blocks to build a castle)

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10
Q

Why do we produce more cells? Aren’t we grown?:

A
  • for repair and maintenance — platelets for example
    • reproduction
      • continuity of life — can trace back to our ancestors
  • growth
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11
Q

Cytokinesis definition

A

the cytoplasm is split into two, for each of the daughter cells

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12
Q

Cytokinesis process

A

When does it begin?: Telophase —After the chromosomes are pulled away to opposite poles so that it can stay on different cells

What is the Proportion of the Mother Cell split?: GENERALLY, Evenly between the daughter cells — the cytoplasm and its contents

Ask Miss

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13
Q

Cytokinesis in animal cells

A

Forming the cleavage furrow — The plasma membrane “bends inwards”, like cinching an hourglass corset
- Done by a ring of contractile proteins, Actin and Myosin, that are similar to proteins that contract the muscle

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14
Q

Cytokinesis in plant cells

A

Cell plate formation — making the cell wall
- prior, doubled the organelles, nucleus, etc. (??)
- golgi apparatus produces the vesicles

  1. microtubules are built into “a scaffolding [metaphorically] straddling along the equator” which is used to create a layer of vesicles. The layer of vesicles form platelike structures (cell plates). The vesicles fuse, creating two layers of plasma membrane along the equator making new plasma membranes for the two daughter cells (”adjacent to the new dividing walls”)
    1. Vacuoles make the cell membrane → the microtubules “scaffold”/build the cell wall
  2. The vesicles bought pasulubong (pectins and other substances, presumably for the cell wall) for the two cells. To divide its contents, exocytosis between the two new membranes (its like a road that leads to two ways). The two daughter cells agrees to give their cellulose to each other. They bring the cellulose to the equator and sent it to the shopee delivery center (ie. the middle lamella)
    1. Why to the equator first then the middle lamella (is the equator a necessary stop or something along the way to the middle lamella?)
  3. Then each cell builds its own cell wall across the equator
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15
Q

Which is more common? Equal or unequal cytokinesis?

A
  • Most cases: Mother cell split into “equal halves” of daughter cells.
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16
Q

Cytokinesis in onion root cells

A
  • new cell walls have divided the cytoplasm equally but not all cookies come out perfect—some may be unequal
    • (so both equal an unequal cell division could happen)
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17
Q

How can unequal cytokinesis occur

A

can only occur with at least 1 nucleus and 1 mitochondrion.
- so only the ones “luck enough” to gain the non-formable elements (such as mitochondria) to survive.

In onion root cells, cytokinesis is typically equal due to the symmetrical formation of the cell plate, which ensures that both daughter cells receive similar amounts of cytoplasm and organelles. However, the process is not always simultaneous due to the inherent variability in the timing of cell plate formation and the complex nature of plant cell cytokinesis.

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18
Q

Why is mitochondria a “non-formable element”??

A

The cell cannot produce the mitochondria. Because in the endosymbiosis, the mitochondria is engulfed by the host cell. It’s is own organism so mitochondria can only be produced from pre-existing mitochondria

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19
Q

Yeast reproduces by budding

A
  1. Nucleus mitosis
  2. The formation of a “small outgrowth of mother cell” called a “bud” (what does that mean? OHH like, see the second step in the illustration, there’s the formation of a mini version of the mommy cell!)
  3. It gains a small portion of the cytoplasm and inherits one of the daughter nucleus from the nucleus mitosis
  4. A division between the two cells (mother and daughter) via the formation of plasma membrane
  5. After the split there would be two scars: Bud scar (Daughter cell), Birth scar (Mother cell)
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20
Q

Oogenesis

A
  • an example of unequal cytokinesis
  • egg cell –> mature ovym
  • genesis: transformation; oo - like yes mom;
  • the production of four offspring: 3 polar bodies and one mature ovum
  • the mature ovum is the bigger cell who gains everything. There are three small polar bodies because its required to split to 2 cells in cell division

The small polar bodies also serve purpose: to make the mature ovum a haploid

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21
Q

The production of sperm

A
  • unlike oogenesis, the cytoplasm is divided equally
  • undergoes meiosis
  • Process: Two stages of division
    • The first results: two daughter cells
    • The second division: four equally sized small cells
    • Final results: the four cells mature into mature sperm cells (why there are so many sperm cells)
  • But in creating a new cell in general, there must always be two outputs.

I think it’s equal because the journey of the sperm to the egg cell is tumultuous and, to increase the chances of fertilization, the sperm count must be plenty.
Plus, they aren’t the ones to nurse the child so they dont need too much volume unlike the egg cell

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22
Q

Nuclear division

A

The splitting of a nucleus to produce two neclei offspring

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23
Q

Anucleate

A
  • an organism or a cell (? wording and knowledge) with no nucleus
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24
Q

Why is it bad to be anucleate?

A
  • Cannot synthesize polypeptides/proteins (which has a lot of essential functions)
    • no instruction
    • nucleolus (from the nucleus) produces the protein-makes/the ribosomes
  • Cannot be able to sustain itself
  • small lifespan
    • e.g. red blood cells can only live for 120 days :(
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25
Q

diploid (2n)

A

A cell with two sets of chromosomes.
- 1 n/set of chromosome from father and mother

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26
Q

Difference between mitosis and meiosis

A

mitosis is for continuity, meiosis is for change
- mitosis: genetically identical, meiosis: genetic variance

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27
Q

What comes before nuclear division?

A

DNA replication
- - Because if you’re producing more of cell X you need the instructions to make cell X not just its packaging.
- So they need the DNA. Meiosis and Mitosis replicates all of the DNA → the daughter cell can perform any function required.

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28
Q

What are chromatids?

A

Two condensed strands of DNA from DNA replication

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29
Q

What does it contain?

A

A long strand of DNA from DNA replication (for yk cell reproduction)

30
Q

Sister chromatids

A

Two strands of the same chromosome that are genetically identical formed by the DNA replication of a chromosome, with both copies joined together by a common centromere
- aka homologous

31
Q

Non-sister chromatids

A

two different homologous chromosomes (one inherited from each parent)

32
Q

When can we see DNA in a microscope?

A
  • It’s only when the DNA is condensed into chromatids could we see it through a light telescope
    • So the elongated DNA still couldn’t be seen
33
Q

Relationship between condensation and cell division

A
  • Condensation is part of cell division.
  • This is to prevent any damage or “tangles” of the elongated DNA by condensing them to compact “packages” (ie. shorter structures)
  • Hence essential for both mitosis and meiosis
34
Q

Brief review on condensation of DNA

A
  • The DNA is coiled or “supercoiled” (I remember there’s a minor discretion with this term) around a histone protein
    • which is in an octet—with one H1 histone protein sealing the coil and making sure it’s still attached to the “backbone” of the coiling (so that it doesn’t ‘fly away’ or curve into a more vulnerable shape ???)
      • nucleosome → solenoid fibre → chromatid → chromosome
35
Q

Similarities between meiosis and mitosis

A
  • both reproduce cells
  • necessary for life (the continuing the existence of species and maintenance of living things)
  • The chromosomes are pulled away by microtubules to opposite poles
36
Q

What does mitosis have but meiosis doesn’t

A

The chromatids are separated

  • Initially not because of the cohesin loops binding them tgt but it gets cut when the microtubules start to “pull”
37
Q

What does meiosis have but mitosis doesn’t

A

The homologous sister chromatids aren’t separated (1st Division of Meiosis)

  • Initially together— bound by a chiasmata (a knot-like structure)
  • But after they “slide away to the ends of the chromosomes”, the chiamsata breaks
38
Q
A
39
Q

What are microtubules?

A
  • A structure that can be rapidly assembled + disassembled (#ezlyfehacks)
  • made from tubulin protein
    • and they’re small, hence microtubules
  • Shaped like a hollow cylinder/tube — hence the name and the name of their proteins
  • Pull on the kinetochores/the hooks attached to the centromeres of the chromatids
  • Has many functions in interphase (look for examples)
    • acting like a cytoskeleton for structure.
  • assembled at their factory—the MTOCs (the MicroTubules Organising Centres) located at the poles of the cell
    • Why at the poles? because they’ll come from the poles and then pull the chromosomes at the equator
    • They form a spindle shaped array at the centre of the cell.
40
Q

What is PMAT?

A

Prophase Metaphase Anaphase telophase
- interphase is not part of cell division but it is the process before that (confirmed with Miss My)
- mitosis

41
Q

Etymology of PMAT

A
  • Pro = before
  • Meta = After
  • Ana = up
  • Telos = finally
  • IPMATI (including interphase)
    • “I’m Pretty Mad at Teachers Infinitely”
42
Q

Process overview of PMAT

A
  • Prophase - condensation for the chromosomes (the coiling)
  • Metaphase - after condensation, “chromosomes released from nucleus”
  • Anaphase - the chromosomes go up to separate poles
  • Telos - the final phase — new nuclei and uncoiled DNA
43
Q

Interphase in Mitosis

A
  • The DNA is already condensed but into two long chromatids
  • To prepare for nuclei division
  • “Chromosomes are scattered around the nucleus, undiscernible (telling which from which)
    • According to Gemini, not yet condensed, that’s why they’re scattered
  • Scattered (chromosome all over the nucleus) → condensation to chromatids
44
Q

Prophase

A
  • DNA packaged into shorter structures
    • Not yet the chromosomes?
    • Bc IIRC there’s more steps to DNA packaging scientists don’t know aboutdon’t understand fully
  • (Nearing the end) The microtubules are being formed @ the MTOCS.
    • Because soon to the next phase
  • At the end, nucleus divides into two (opposite poles)
    • To pull chromosomes for the two daughter cells
  • The nuclear membrane disintegrates
45
Q

Metaphase

A
  • “fastening the seatbelt”
  • Microtubules PREPARE to pull away the chromosomes to the opposite poles
    • the microtubules attach to the centromeres of the chromosomes
    • The test of strength/”under tension” (metaphor): Thor, aka the microtubules, has to test his strength by lifting the Hammer (pulling away the chromosomes).
      • If the microtubules are “worthy”/”the appropriate/chosen one” (assembled correctly), they can break the cohesin pulls by pulling the chromosomes away
  • As preparation, at the end of metaphase, the chromosomes are lined up at the equator
46
Q

Anaphase

A
  • “Take off” ✈️
  • Chromosomes begin to pull away to the opposite poles
    • the kinetochrome shortens the microtubules
      • detaches tubulin proteins of the microtubules to move the chromosomes to the opposite poles
        • Kineto - movement (engine)
        • chrome - as in chromosomes
        • because it helps make the chromosomes move
  • At the end, they landed to their destination (the poles aka the two nuclei) but not yet decondensed (has not yet “loaded off”)
47
Q

Telophase

A
  • “Leaving the plane”
  • Decondensation + the splitting of the cytoplasm
    • First, they leave by “seat”
      • The chromosomes are packed into “tight groups” near the MTOC (why?) —> decondense as chromatin → “disperse” / scatter inside the nucleus (like how the attendees of the plane leave as a group but split when they enter the airport)
  • The chromosomes found their “hotels”/new home (respective nuclei)
48
Q

Interphase (II)

A
  • Chilling in the hotel room” / “making themselves home”
  • Protein-making!
    • To produce the protein needed to make the different components of the cell.(making the furniture)
    • So transcription and translation
  • The cell gets a growth spurt / G1 Phase
    • Doubles in size prior to the next mitosis
      • Enzymes and other proteins produced that contribute in growth (e.g EGF which starts cell growth + cell proliferation → inc in cell count)
      • Organelle synthesis
        • e.g. ribosomes + the rER make proteins so they can make the proteins responsible for cell growth, mitochondria gives energy to the cell for these processes
49
Q

Homologous

A
  • the same sequence of genes (i.e. genetically identical), paired up
    • But allele wise, they can be different
    • Same positions on the chromosome
50
Q

Chromosomes

A

very long but “condensed” DNA molecule, has some associated proteins

51
Q

Alleles

A

variations of the same gene
E.g. Gene = eye colour; Alleles = brown, green, blue eye colors

52
Q

Haploid (n)

A
  • something that has a single set of chromosomes, non-homologous
    • Non-homologous: Each chromosome is unique from one another
    • “something”= a nucleus, cell or organism (basically anything that can contain genetic material)
53
Q

Diploid (2n)

A
  • something that has a double set of chromosomes
    • Chromosomes in couples or pairs.
      • But like couples, the two chromosomes are not the same.
      • But they “complement” each other (complementary pairs)
        • From the mother and the father respectively
        • They are similar — same length, centromere position and gene locations
54
Q

On chromosomes:

A
  • Has ~1000 genes in a linear sequence
  • Gene sequence does not change in replication
    • The order of genes does not change (it’s like their class number in class)
  • But, the bases of indv. genes themselves can change/mutate
    • The process of replication is imperfect, when the DNA is unraveled, it is vulnerable to all sorts of changes
    • Results in new alleles → more variations of the same gene
    • It is like the students’ academic performances can change but their Class Number could change
55
Q

What is recombination?

A
  • Recombination is “the reshuffling of alleles to create new combinations”
    • During meiosis.
    • What does it mean by “new combinations”?? “Reshuffling” would imply that the places of the alleles would change (but not the gene itself, no?)
      • So the placement of alleles would determine the phenotype expression would be exhibited(e.g. brown eyes)
56
Q

What does recombination explain?

A

Explains how chromosomes can be homologous but not identical

57
Q

On diploid cells production specifically,

A
  • Produced during sexual reproduction/sex.
  • sperm cell + egg cell (fertilization) = diploid cell (the zygote)
  • Every other cell came from the zygote (see the presentation on cell differentiation, the cells divide and divide and then differentiate) — hence all the normal body cells are diploid
    • The zygote cell divides but without largening → morula develops into → blastocyst → inner cell mass differentiates
58
Q

On haploid cells production, specifically,

A
  • The gametes are haploid because 1/2 + 1/2 = 2 | or n + n = 2n
  • So in gamete production would produce haploid cells
  • Why should they be haploids?
    • Because it would produce 4n gametes (tetraploid → 2n + 2n)
    • 4n + 4n gametes = 8n offspring (octoploid)
    • Too many chromosomes result into aneuploidy
      • Effects: birth defects, death
      • Small stature
  • Hence, there must be a reduction process to half the amount of chromosomes (2n → n)
    • Meiosis is the reduction division
    • Counteracts the “doubling effect” of the gametes fusing
      • so that when they fuse its n + n = 2n instead of 2n + 2n = 4n. ?
59
Q

Cytokinesis root tips

A
  • In root tips, the cytoplasm (hence its contents) are divided equally because it’s needed for them to grow (e.g. to have a nucleus)
    • The root cells are all apportioned equally because they’re differentiated in the same way
60
Q

What are considered the opposite poles of the cell?

A

Literally the opposite ends of the cell.
- Chromosomes going to the opposite poles -> Anaphase

61
Q

Where does Anaphase occur?

A

inside the cell but not inside the nucleus

62
Q

What happens to the nucleus during Anaphase and Metaphase?

A

There is no more nucleus at these two stages?
- Why?:
- In prophase, the nuclear envelope (aka its “body”) is broken down
- allows for the spindle fibres to access and attach to the chromosomes for Metaphase so that they can be pulled away in Anaphase

63
Q

How does the nucleus stop the chromosomes from attaching to the spindle fibres?

A

Because the father (the Nuclear envelope) is a barrier for their love </3.
- Basically like a wall that cages these chromosomes! Inaccessible to outside
- Plus I doubt the chromosomes can go through the nuclear pores because they cannot fit—only the mRNA can go through there I think

64
Q

(For my understanding) How and why does division go from “the inside going out”?

A

Because the smallest or “most inner” divides/reproduces/replicates first.
- The DNA (which is in the nucleus) replicates in S-phase of the interphase –> the nucleus divides in telophase -> the cytokinesis/(“the whole cell”) divides at the end of telophase.

65
Q

What is the difference between Anaphase I and Anaphase?

A

In Anaphase (mitosis), the chromatids already separate into two poles (like Anaphase II)

In Anaphase I, chromosomes in chromosome pairs are pulled away from each other

66
Q

Metaphase vs Metaphase I

A

In metaphase, the chromosomes line up in the centre of the cell but they do not line up in pairs. (like in Metaphase II)

In Metaphase I, the chromosome pairs line up at the centre

In mitosis, the daughter cells would not be halpoids so there’s no reason for them to pair up and go into a second dicision which is why they lined up as such in Metaphase

67
Q

Anaphase vs Anaphase I

A

In anaphase I, the homoloogous chromosomes separate from each other

In Anaphase, sister chromatids separate from each other and move to opposite poles

68
Q

Telophase vs Telophase I

A

In telophase, 2 diploid nuclei are formed

Can I see the math on this?

69
Q

Homologous definition

A

Chromosomes with the same length and the same gene locations

70
Q
A