Core Practical 7

Question 1

Safety

Answer 1

avoid cuts by sharp dissection tubes .keep in a ordered fashion in tray when not in use
wash hands with soap after handling insects (gloves)
Methylene blue wear eye protection and wash hand if any contact

Question 2

ethical considerations

Answer 2

advancing knowledge must outweigh harm to a living thing
personal experience of the nature of fresh tissues and variation between individual animals
fosters an knowledge of the organism and develops manual skills -cannot be fully provided by other means

Question 3

rough procedure

Answer 3

pores within had impermeable chitinous exoskeleton
located on thoracic and abdominal segments
cut off wings
pin to wax or cork board .
cut along each side of exoskeleton above spiracles
Keep tip of scissors raised to avoid cutting through body tissues.Remove exoskeleton .Flood dissection with water to make any air filled structure stand out -have a silvery appearance under water .Remove a spiracle add water add cover slip observe under microscope .Remove tracheae add few drops of methyl blue outside coverslip and observe

Question 4

what do the rings of chitin do

Answer 4

hold tracheae open if the insect moves and the tracheae become squashed or deformed -support airways

Question 5

how to make the practical ethically responsible

Answer 5

made sure we understood how to complete the dissection -ensure was successful so the maximum learning could be gained
one locust each so minimal amount died
only made necessary cuts