Controlling Microbial Growth Flashcards
Exam 2
What are the 3 methods of microbial growth control?
- physical
- chemical
- mechanical
What are the 2 types of physical control for microbial growth?
Provide Examples
- heat (dry and moist)
- radiation (ionizing and non-ionizing)
What is the pro/con of using dry heat for microbial control?
- pro: inexpensive
- con: lose use of item
What are 2 examples of using dry heat for microbial control?
- dry oven
- incineration
What are 2 types of moist heat for microbial control?
- pressurized (sterilization)
- non-pressurized
What is used for pressurized moist heat microbial growth control?
Be specific
Autoclave
- PSI: 15
- Temp: 121C
- Time: 10 - 40 min
What are examples of non-pressurized moist heat for microbial growth control?
- hot water
- boiling water
- pasteurization
What is a requirement for pasteruization?
must maintain flavor and nutritional value
What are 2 methods for pasteurization?
Provide specifications
- batch method: 63.3C for 30 mins
- flash method: 75.5C for 15 sec
What are examples of ionizing radiation?
- x-ray
- cathode ray
- gamma ray
- microwave (food irradiation)
What is an example of non-ionizing radiation?
UV-rays
What is an example of Chemical control of microbial growth?
Chemotherapy: using a chemotherapeutic agent
What is an example of mechanical control of microbial growth?
HEPA (High Efficiency Particulate Air)
What elements are used to control microbial growth?
Provide an example
- Halogens
- ex: iodine
- ex: chlorine
What is a tincture of iodine?
iodine + alcohol
What is a iodophor of iodine?
What is it’s benefit?
- iodine + organic solvent
- doesn’t irrirate skin
What are 3 examples of how Chlorine is used to control microbial growth?
- swimming pools
- hypochlorite (Chlorox)
- Chloramine (chlorine + ammonia (amino)
What do phenols/phenolics do?
kill vegetative cells, not spores
What is another name for Chlorahexidine?
Biguanides
What is Oligodynamic Action?
when heavy metals (copper, gold, zinc silver) kill microbes
What is the name of the first synthetic drug without heavy side effects?
Salvarsan
What is infection?
microbial replication/multiplication
What is disease?
the spread of the replicated microbes to tissue and organs
What is it called when microbes start to cause permanent damage?
sequelae