Compement System

Question 1

What Complement protein is both a chemotaxin and an anaphylatoxin?

Answer 1

C5a

Question 2

What complement is associated with opsonization?

Answer 2

C3b

Question 3

Complement proteins responsible for the activation of mast cells

Answer 3

c3a, c4a, c5a`

Question 4

The Classical pathway is activated by what reaction?

Answer 4

Ag-Ab reaction

Question 5

The Alternative pathway is activated by what reaction/factor?

Answer 5

Bacterial endotoxin (LPS, G- bac)
Fungal cell wall (zymosan)
Cobra venom
Virus, Parasite
IgA

Question 6

What is the recognition unit in Classical pathway?

Answer 6

C1 complex

Question 7

What are the activation unit in the complement cascade?

Answer 7

c4-c5

Question 8

C1q in IgG binds to?

Answer 8

CH2

Question 9

C1q in IgM binds to?

Answer 9

CH2/CH4

Question 10

The Lectin pathway is activated by?

Answer 10

MBL in liver
Neisseria, candida, Salmonella, Mannose, Glucose

Question 11

The C3bBb is stabilized by what substance?

Answer 11

Properdin

Question 12

Factor B is cleaved into Ba and Bb withe the help of what?

Answer 12

Factor D

Question 13

Complement proteins are produced where?

Answer 13

liver

Question 14

C1 Def

Answer 14

Lupus-like syndrome

Question 15

C2 Def

Answer 15

most common
artherosclerosis

Question 16

C3 def

Answer 16

Glomerulonephritis

Question 17

C4 Deficiency

Answer 17

Lupus-like syndrome

Question 18

C5, 6, 7, 8 DEFICIENCY

Answer 18

NEISSERIA SPP

Question 19

C9 DEFICIENCY

Answer 19

CELL LYSIS

Question 20

PROPERDIN DEFICIENCY

Answer 20

NEISSERIA SPP

Question 21

FACTOR H & I DEFICIENCY

Answer 21

PYOGENIC INFECTION

Question 22

MBL DEFICIENCY

Answer 22

PNEUMOCOCCAL INFX, SEPSIS, NESISSERIA INX

Question 23

MASP-2 DEFICIENCY

Answer 23

PNEUMOCOCCAL INFX

Question 24

DAF DEFICIENCY

Answer 24

PNH

Question 25

MIRL/CD59 DEFICIENCY

Answer 25

PNH

Question 26

C1 INH DEFICIENCY

Answer 26

HANE (HEREDITARY ANGIONEUROTIC EDEMA) DUE TO C2b

Question 27

This inactivates MASP2, dissociates C1r and C1s from C1q

Answer 27

C1 inhibitor

Question 28

This cleaves C3b and C4b

Answer 28

Factor I

Question 29

Inactivates C4b by being a cofactor to Factor I, also prevents the binding of factor B by binding C3b

Answer 29

factor H

Question 30

Acts as a cofactor to Factor I to inactivate C4b

Answer 30

C4 binding protein

Question 31

Prevents the attachment of C5b67 complex to membranes

Answer 31

S protein (vitronectin)

Question 32

Blocks the C8, prevents the formation of MAC

Answer 32

HRF (Homologous restriction factor)

Question 33

Blocks the assembly of MAC by binding C8 and C9

Answer 33

CD59/MIRL (membrane Inhibitor of reactive Lysis)

Question 34

Prevents C3 convertase by removing C2a and Bb in the membrane

Answer 34

CD55/DAF

Question 35

These components have standardized reagents in RID

Answer 35

C1q, C3, C4, C5, Factor B, H, I

Question 36

This test measures the amount of px serum required to lyse 50% of a standardized concentration of an Ab sensitized sheep rbcs

Answer 36

Hemolytic Titration (CH50) Assay

Question 37

this is based on the lysis of liposomes that release an enzyme when lysed

Answer 37

Liposome lysis

Question 38

CH50 Assay measures what complement proteins?

Answer 38

C1-C9 proteins

Question 39

What is the indicator of complement activation used in ELISA?

Answer 39

AHG to C9 labeled with ALP

Question 40

In AH50 Assay, what is added to the buffer which chelated calcium?

Answer 40

MgCl and EGTA (Ethylene Glycol)

Question 41

What indicator is used in AH50 Assay?

Answer 41

Rabbit RBCs

Question 42

This test can detect C3bBb

Answer 42

ELISA