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Immunoserology > Antibodies > Flashcards

Antibodies Flashcards

1
Q

What do you call specific glycoproteins produced in response to an antigenic stimulation?

A

Immunoglobulins

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2
Q

What part of the antibody determines the immunoglobulin class?

A

heavy chain

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3
Q

The Heavy chain is encoded by what chromosome?

A

Chromosome 14

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4
Q

The light chain is encoded in what chromosome?

A

Kappa (ch 2) and Lambda (ch 22)

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5
Q

This links the H-H chain, H-L chain

A

Disulfide bonds

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6
Q

L-L chain is only found in where?

A

Bence Jones protein

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7
Q

This provides flexibility of the antibody due to Proline

A

Hinge region

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8
Q

The variable region contains what region?

A

Fab region (PARATOPE), antigen binding site

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9
Q

This determines the specificity of the antibody

A

Hypervariable region

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10
Q

Constant region contains the _________ region

A

Fc region (fragment crystallizable)

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11
Q

This is composed of 1 variable and 1 constant domain

A

Light chain

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12
Q

This is composed of 1 variable and 3/4 constant domain

A

Heavy chain

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13
Q

Rodney Porter described Ab structure using _____

A

papain

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14
Q

Rodney Porter described Ab structure using Papain, yields 3 products, what are those?

A

“ABOVE”

1 Fc, 2 Fab

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15
Q

Alfred Nisonoff described Ab structure using Pepsin, yields 2 products, what are those?

A

“BELOW”

1 Fc, 1 F(ab)2

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16
Q

What is considered an incomplete immunoglobulin?

A

IgG

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17
Q

What is the main antibody for SECONDARY immune response?

A

IgG

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18
Q

What IgG best in crossing the placenta?

A

IgG1

“PG 134”

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19
Q

What IgGs can fix complement?

A

“GC 312”

IgG3 > IgG1 > IgG2

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20
Q

IgG is best in what reaction?

A

Pecipitation

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21
Q

IgG responds best in what type of antigen?

A

Protein antigens

22
Q

What Ig is considered the Macroglobulin or Complete immunoglobulin?

A

IgM

23
Q

IgM is the main Ab for ________ immune response, and indicate acute infection

A

PRIMARY/EARLY

24
Q

IgM is effective in what type of reaction?

A

Agglutination reaction

25
Q

This Ig is formed in response to G- bacteria and endotoxin neutralization

A

IgM

26
Q

IgM beest responds to what to of antigens?

A

Carbohydrate

27
Q

RF and Cold Agglutinins are what type of Ig?

A

IgM

28
Q

What Ig that is monomer in serum and dimer on secretions?

A

IgA

29
Q

Ig present in secretions, saliva, milk?

A

IgA

30
Q

What Ig is present in B cell surface?

A

IgD

31
Q

IgE, AKA?

A

REAGIN

32
Q

This Ig binds to mast cells and triggers hypersensitivity

A

IgE

33
Q

What Ig also plays a role in defense to parasites?

A

IgE

34
Q

This antibody is present in healthy indiv, heavy chain that determines the Ig class

A

Isotype

35
Q

A type of Ab that has a variation in the constant region

A

Allotype

36
Q

What type of Ab that has a difference in the variable region that gives its specificity?

A

Idiotype

37
Q

IgG half life?

A

23 days (21-25d)

38
Q

IgM half life?

A

6 days

39
Q

IgA half life?

A

5 days

40
Q

IgD half life?

A

1-3 days

41
Q

IgE half life?

A

2-3 days

42
Q

IgM MW?

A

900,000 mw

43
Q

IgG MW?

A

150,000 mw

44
Q

Sedimentation rate:
IgG
IgM
IgA
IgD
IgE

A

IgG: 7s
IgM: 19s
IgA: 7s,11s
IgD: 7s
IgE: 8s

45
Q

Ig present in colostrum?

A

IgA, IgG

46
Q

What are fused B cells and myeloma cells capable of secreting antibodies idefinitely?

A

Hybridoma cells

47
Q

In monoclonal Abs, the B cell is fused to a myeloma cell by using what substance?

A

PEG

48
Q

What media is used culturing hybridoma cells?

A

HAT media
(Hypoxanthine Aminopterine Thymidine)

49
Q

A substance in the media used for DNA production in creating monoclonal Abs

A

Thymidine, Hypoxanthine

50
Q

This susbstance is used to block the production of new nucleotides for the culture of monoclonal Abs

A

Aminopterin

51
Q
A

Immunoserology (20 decks)

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