Antibodies

Question 1

What do you call specific glycoproteins produced in response to an antigenic stimulation?

Answer 1

Immunoglobulins

Question 2

What part of the antibody determines the immunoglobulin class?

Answer 2

heavy chain

Question 3

The Heavy chain is encoded by what chromosome?

Answer 3

Chromosome 14

Question 4

The light chain is encoded in what chromosome?

Answer 4

Kappa (ch 2) and Lambda (ch 22)

Question 5

This links the H-H chain, H-L chain

Answer 5

Disulfide bonds

Question 6

L-L chain is only found in where?

Answer 6

Bence Jones protein

Question 7

This provides flexibility of the antibody due to Proline

Answer 7

Hinge region

Question 8

The variable region contains what region?

Answer 8

Fab region (PARATOPE), antigen binding site

Question 9

This determines the specificity of the antibody

Answer 9

Hypervariable region

Question 10

Constant region contains the _________ region

Answer 10

Fc region (fragment crystallizable)

Question 11

This is composed of 1 variable and 1 constant domain

Answer 11

Light chain

Question 12

This is composed of 1 variable and 3/4 constant domain

Answer 12

Heavy chain

Question 13

Rodney Porter described Ab structure using _____

Answer 13

papain

Question 14

Rodney Porter described Ab structure using Papain, yields 3 products, what are those?

Answer 14

“ABOVE”

1 Fc, 2 Fab

Question 15

Alfred Nisonoff described Ab structure using Pepsin, yields 2 products, what are those?

Answer 15

“BELOW”

1 Fc, 1 F(ab)2

Question 16

What is considered an incomplete immunoglobulin?

Answer 16

IgG

Question 17

What is the main antibody for SECONDARY immune response?

Answer 17

IgG

Question 18

What IgG best in crossing the placenta?

Answer 18

IgG1

“PG 134”

Question 19

What IgGs can fix complement?

Answer 19

“GC 312”

IgG3 > IgG1 > IgG2

Question 20

IgG is best in what reaction?

Answer 20

Pecipitation

Question 21

IgG responds best in what type of antigen?

Answer 21

Protein antigens

Question 22

What Ig is considered the Macroglobulin or Complete immunoglobulin?

Answer 22

IgM

Question 23

IgM is the main Ab for ________ immune response, and indicate acute infection

Answer 23

PRIMARY/EARLY

Question 24

IgM is effective in what type of reaction?

Answer 24

Agglutination reaction

Question 25

This Ig is formed in response to G- bacteria and endotoxin neutralization

Answer 25

IgM

Question 26

IgM beest responds to what to of antigens?

Answer 26

Carbohydrate

Question 27

RF and Cold Agglutinins are what type of Ig?

Answer 27

IgM

Question 28

What Ig that is monomer in serum and dimer on secretions?

Answer 28

IgA

Question 29

Ig present in secretions, saliva, milk?

Answer 29

IgA

Question 30

What Ig is present in B cell surface?

Answer 30

IgD

Question 31

IgE, AKA?

Answer 31

REAGIN

Question 32

This Ig binds to mast cells and triggers hypersensitivity

Answer 32

IgE

Question 33

What Ig also plays a role in defense to parasites?

Answer 33

IgE

Question 34

This antibody is present in healthy indiv, heavy chain that determines the Ig class

Answer 34

Isotype

Question 35

A type of Ab that has a variation in the constant region

Answer 35

Allotype

Question 36

What type of Ab that has a difference in the variable region that gives its specificity?

Answer 36

Idiotype

Question 37

IgG half life?

Answer 37

23 days (21-25d)

Question 38

IgM half life?

Answer 38

6 days

Question 39

IgA half life?

Answer 39

5 days

Question 40

IgD half life?

Answer 40

1-3 days

Question 41

IgE half life?

Answer 41

2-3 days

Question 42

IgM MW?

Answer 42

900,000 mw

Question 43

IgG MW?

Answer 43

150,000 mw

Question 44

Sedimentation rate: IgG IgM IgA IgD IgE

Answer 44

IgG: 7s IgM: 19s IgA: 7s,11s IgD: 7s IgE: 8s

Question 45

Ig present in colostrum?

Answer 45

IgA, IgG

Question 46

What are fused B cells and myeloma cells capable of secreting antibodies idefinitely?

Answer 46

Hybridoma cells

Question 47

In monoclonal Abs, the B cell is fused to a myeloma cell by using what substance?

Answer 47

PEG

Question 48

What media is used culturing hybridoma cells?

Answer 48

HAT media (Hypoxanthine Aminopterine Thymidine)

Question 49

A substance in the media used for DNA production in creating monoclonal Abs

Answer 49

Thymidine, Hypoxanthine

Question 50

This susbstance is used to block the production of new nucleotides for the culture of monoclonal Abs

Answer 50

Aminopterin