Agglutination, Precipitation, Electrophoresis, Complement Fixation

Question 1

Insoluble particles react with an Ab which results in aggregation/clumping

Answer 1

Direct Agglutination

Question 2

This is used to detect if an Ab is present. The reaction where Ag is attached to a carrier particle.

Answer 2

Indirect/Passive Agglutination

Question 3

This is used to detect if antigen is present in the sample. The antibody is attached to a carrier particle.

Answer 3

Reversed Passive Agglutination

Question 4

This is based on competition between particulate and soluble Ag for limited Ab-binding sites. (eg. Pregnancy test)
(+) No agglutination

Answer 4

Agglutination Inhibition

Question 5

This is used to detect patient Abs while utilizing RBCs as indicator cells, has the same principle with agglutination inhibition.

Answer 5

Hemagglutination inhibition

Question 6

This test detect incomplete Abs

Answer 6

AHG

Question 7

Used to detect IN VIVO SENSITIZATION

Answer 7

DAT

Question 8

Used to detect IN VITRO SENSITIZATION

Answer 8

IAT

Question 9

What is the bacterium used in Coagglutination test as a carrier?

Answer 9

S. aureus (protein A)

Question 10

The IMPACT/Immunoassay by PArticle Counting uses what substance as a carrier?

Answer 10

Latex

Question 11

Many tiny agglutinates, many free cells, turbid background

Answer 11

W+ (<25%)

Question 12

Many Medium-sized agglutinates, moderate free cells, clear background

Answer 12

2+ (50%)

Question 13

This is a combination of soluble Ag with a soluble Ab to produce insoluble complexes

Answer 13

Precipitation

Question 14

Pro-zone and Post-zone phenomenon leads to a _________ reaction

Answer 14

false (-) reaction

Question 15

What is a post zone phenomenon?

Answer 15

Excess Ag

Question 16

Single diffusion, Single dimension

Answer 16

Oudin

Question 17

Single diffusion, Double dimension

Answer 17

Radial Immunodiffusion

Question 18

Double diffusion, Single dimension

Answer 18

Oakley & Fulthrope

Question 19

Double diffusion, Double dimension

Answer 19

Ouchterlony & Elek

Question 20

The Mancini (endpoint) is read at what time?

Answer 20

1-3days
IgG: 1 day
IgM: 50-57 hrs

Question 21

Fahey/McKelvy (Kinetic) is read after what time?

Answer 21

18 hrs

Question 22

Lytic Complement Fixation test detects what immunoglobulins?

Answer 22

IgG, IgM