Antigen Presentation

Question 1

The MHC Class I presentation is also referred as

Answer 1

Endogenous/Cytosolic Pathway

Question 2

The MHC Class II presentation is also referred as

Answer 2

Exogenous/Endocytic Pathway

Question 3

Class I MHC is present in what cells?

Answer 3

All nucleated cells, plt, rbc,

Question 4

Class I MHC has what chain?

Answer 4

Alpha chain, B2 microglobulin

Question 5

Class II MHC has what chain?

Answer 5

Beta chain

Question 6

Class II MHC is present on what type of cells?

Answer 6

APC, mononuclear cells

Question 7

What is the other term of MHC found in humans?

Answer 7

HUman Leukocyte Antigen

Question 8

HLA is present or produced in what chromosome?

Answer 8

Chromosome 6, short arm”p”

Question 9

MHC Class I has what type of HLA?

Answer 9

HLA A,B,C

Question 10

MHC Class I pairs with type of CD marker?

Answer 10

CD8 T cells (cytotoxic)

Question 11

MHC Class II pairs with type of CD marker?

Answer 11

CD4 T cells

Question 12

CD8 T cells are activated by what cytokine?

Answer 12

IL-2

Question 13

What MHC class is used during viral replication, tumor, and graft rejection?

Answer 13

MHC I

Question 14

What HLA is present in MHC Class II?

Answer 14

HLA D, DP, DQ, DR

Question 15

Th cells become Th1 with the help of what cytokine?

Answer 15

IL-12

Question 16

Th1 producces what cytokine?

Answer 16

IFN-y

Question 17

Th1 produces IFN-y and helps in antibody secretion through?

Answer 17

Opsonization

Question 18

Th2 is activated by what interleukin?

Answer 18

IL-4

Question 19

Th2 produces what cytokines?

Answer 19

IL4 - IgE,. IgG4, isotope switching
IL5 - eosinophil

Question 20

Th2 is for mast cell degranulation and?

Answer 20

helminths destruction

Question 21

What MHC class directed to proteins, complement, and cytokines?

Answer 21

Class 3

Question 22

Class III MHC include what kinds of proteins/cytokines?

Answer 22

C2, C4, Factor B, TNF

Question 23

HLA B8 is associated with what disease/s?

Answer 23

Myasthenia Gravis, Addison’s disease, Grave’s disease

“8te my energy, adrenals, and thyroid”

Question 24

HLA B27 is associated with what disease/s?

Answer 24

“PAIR”
P- Psoriatic Arthritis
A - ANKYLOSING SPONDILITIS
I - Infectious Bowel Disease (Crohn’s, Ulcerative colitis, etc.)
R - Reactive Arthritis (Writer Syndrome)

Question 25

HLA DQ2 is associated with what disease/s?

Answer 25

Celiac Disease, DM type 1

Question 26

HLA DQ8 is associated with what disease/s?

Answer 26

DM type 1, Celiac Disease

Question 27

HLA DR3 is associated with what disease/s?

Answer 27

SLE, DM type 1, Grave's dse

Question 28

HLA DR4 is associated with what disease/s?

Answer 28

RA, DM type 1

Question 29

An in vitro test that mimics the in vivo condition of transplantation

Answer 29

Mixed Lymphocyte Reaction

Question 30

In Mixed Lymphocyte Reaction, lymphocyttes are inactivated by?

Answer 30

Mytomycin C, Irradiation

Question 31

The amount of proliferation in Mixed Lymphocyte Reaction test is measured with the help of?

Answer 31

3H-labeled thymine (tritium) *measured using liquid scintillation counter

Question 32

Mixed Lymphocyte Reaction is best for what kind of tests?

Answer 32

BM graft, living related donors, HLA-B-D

Question 33

A kind of Tissue Matching test where PCR, and enzymes are used to cleave DNA for patterns and then assessed through comparison of fragments

Answer 33

RFLP (Restriction Fragment length Polymorphism)

Question 34

What dye is used in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?

Answer 34

Eosin Red, Trypan Blue

Question 35

What type of sample is used in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?

Answer 35

ACD or heparinized blood (1-2 mL)

Question 36

What microscope is used in reading the plates in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?

Answer 36

Inverted Phase COntrast Microscope

Question 37

Characteristics of uninjured cells in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?

Answer 37

small, bright, refractile

Question 38

Characteristics of injured cells in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?

Answer 38

flattened, large, dark, non-refractile (+) HLA Ag

Question 39

HLA-DR typing requires the ID of what cell?

Answer 39

Bcell

Question 40

In HLA-DR typing, B cell is separated from the T cell using what substance?

Answer 40

Nylon wool