Antigen Presentation Flashcards
The MHC Class I presentation is also referred as
Endogenous/Cytosolic Pathway
The MHC Class II presentation is also referred as
Exogenous/Endocytic Pathway
Class I MHC is present in what cells?
All nucleated cells, plt, rbc,
Class I MHC has what chain?
Alpha chain, B2 microglobulin
Class II MHC has what chain?
Beta chain
Class II MHC is present on what type of cells?
APC, mononuclear cells
What is the other term of MHC found in humans?
HUman Leukocyte Antigen
HLA is present or produced in what chromosome?
Chromosome 6, short arm”p”
MHC Class I has what type of HLA?
HLA A,B,C
MHC Class I pairs with type of CD marker?
CD8 T cells (cytotoxic)
MHC Class II pairs with type of CD marker?
CD4 T cells
CD8 T cells are activated by what cytokine?
IL-2
What MHC class is used during viral replication, tumor, and graft rejection?
MHC I
What HLA is present in MHC Class II?
HLA D, DP, DQ, DR
Th cells become Th1 with the help of what cytokine?
IL-12
Th1 producces what cytokine?
IFN-y
Th1 produces IFN-y and helps in antibody secretion through?
Opsonization
Th2 is activated by what interleukin?
IL-4
Th2 produces what cytokines?
IL4 - IgE,. IgG4, isotope switching
IL5 - eosinophil
Th2 is for mast cell degranulation and?
helminths destruction
What MHC class directed to proteins, complement, and cytokines?
Class 3
Class III MHC include what kinds of proteins/cytokines?
C2, C4, Factor B, TNF
HLA B8 is associated with what disease/s?
Myasthenia Gravis, Addison’s disease, Grave’s disease
“8te my energy, adrenals, and thyroid”
HLA B27 is associated with what disease/s?
“PAIR”
P- Psoriatic Arthritis
A - ANKYLOSING SPONDILITIS
I - Infectious Bowel Disease (Crohn’s, Ulcerative colitis, etc.)
R - Reactive Arthritis (Writer Syndrome)
HLA DQ2 is associated with what disease/s?
Celiac Disease, DM type 1
HLA DQ8 is associated with what disease/s?
DM type 1, Celiac Disease
HLA DR3 is associated with what disease/s?
SLE, DM type 1, Grave’s dse
HLA DR4 is associated with what disease/s?
RA, DM type 1
An in vitro test that mimics the in vivo condition of transplantation
Mixed Lymphocyte Reaction
In Mixed Lymphocyte Reaction, lymphocyttes are inactivated by?
Mytomycin C, Irradiation
The amount of proliferation in Mixed Lymphocyte Reaction test is measured with the help of?
3H-labeled thymine
(tritium)
*measured using liquid scintillation counter
Mixed Lymphocyte Reaction is best for what kind of tests?
BM graft, living related donors, HLA-B-D
A kind of Tissue Matching test where PCR, and enzymes are used to cleave DNA for patterns and then assessed through comparison of fragments
RFLP (Restriction Fragment length Polymorphism)
What dye is used in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?
Eosin Red, Trypan Blue
What type of sample is used in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?
ACD or heparinized blood (1-2 mL)
What microscope is used in reading the plates in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?
Inverted Phase COntrast Microscope
Characteristics of uninjured cells in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?
small, bright, refractile
Characteristics of injured cells in the Complement Mediated Microlymphocytotoxicity test, aka, CDC (Complement-dependent Cytotoxicity)?
flattened, large, dark, non-refractile
(+) HLA Ag
HLA-DR typing requires the ID of what cell?
Bcell
In HLA-DR typing, B cell is separated from the T cell using what substance?
Nylon wool
