Clinical Mircobbiology

Question 1

How do you effectively treat the patient?

Answer 1

Culture of organisms from patient specimens
Classification and ID of isolated microorganisms
Prediction and interpretation of antimicrobial susceptibility patterns
- How do the bugs cause a disease and effect our health

Question 2

Microbiota

Answer 2

All the living bacteria

Question 3

Mircrobiome

Answer 3

All the genes and function

How it effects lifestyle, genotype

Question 4

Virome

Answer 4

All the viruses in the gut

Question 5

Born and Die?

Answer 5

Born 100% human

Die 90% microbial

Question 6

How do we measure?

Answer 6

Intestinal content
Extract gDNA
PCR
Pyrophosphate sequencing
NOT QUICK

Question 7

Five Fundamental Biological Questions

Answer 7

What is the identity of the microbes that populate the host?
What are these microbes doing?
How is the host responding?
What are the forces that maintain equilibrium among the populations?
What are the unique characteristics of each individual?

Question 8

Oral Cavity Bacteria

Answer 8

10^9

Question 9

Esophagus Bacteria

Answer 9

10^2

Question 10

Stomach Bacteria

Answer 10

10^3

Question 11

Small Intestine Bacteria

Answer 11

10^7

Question 12

Colon Bacteria

Answer 12

10^12

Question 13

What is Enterotype

Answer 13

Classification based on bacteriological composition of the gut microbiome
- Might play a role in pharmacological action