Clinical Lab Flashcards
Patients To Drug
Sample is plated
18-24 hrs later, plate is examined
Id preformed (18-24 or 3-4h)
Antimicrobial sensitivity (18-24 or 3-4)
Why culture workup?
Assess and quantitate normal microbiome
Differentiate potential pathogens from indigenous bacterial microbiome
Complete identification and susceptibility of potential pathogen
Throat cultures used for diagnosis of?
Group A Strep
Nasopharynx cultures used for diagnosis of?
Pertussis (whooping cough)
Lower Respiratory Tract culture used for diagnosis of?
Bronchitis or pneumonia
Clostridium difficile
Gram Positive
Anaerobe
Spore-forming
Presumptive ID
Look at it and know what it is
Definitive ID
Biochemical ID system
Based on cultures
Macroscopic characteristics
Color
Colonial Texture
Shape
Odor
CLSI
Clinical and Lab Standards Institute
Non-government
Antimicrobial objective: least toxic, most cost effective, clinically appropriate
Breakpoint Definied
Discriminating concentration used in the interpretation of results of susceptibility testing to define isolates as S, I or R
Breakpoint
May change
Vary from country to country
Vary between agency (FDA vs CLSI)
Susceptible Define
Antimicrobial agent MAY be appropriate choice for treating infection
Intermediate
Number of possibilities
May help or may not
Resistant
Antimicrobial may not be appropriate
