Chapter Eight: Using Mutations to Study Genes Flashcards

1
Q

reveals whether two mutations are in a single gene or different genes

A

complementation testing

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2
Q

used phage T4 to test if recombination takes place between different mutations in the same gene

A

Seymour Benzer

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3
Q

sites within a gene that mutate more often than others

A

mutation hotspots

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4
Q

mutation hotspots suggest that some nucleotides are ___ than others

A

more susceptible to mutations

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5
Q

used bread mold to study the relationship between genes and enzymes

A

Bealde and Tatum

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6
Q

wild-type strain that grows in minimal media without nutritional supplements

A

phototroph

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7
Q

mutant strain that cannot grow in minimal media, requires supplementation

A

auxotroph

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8
Q

three components of a complete amino acid

A
  1. carboxylic acid (-COOH)
  2. R group connector (-CHR)
  3. amino group (-NH2)
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9
Q

side chain unique to each amino acid

A

R group

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10
Q

R group determines ___ of protein

A

chemical properties

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11
Q

amino acids are linked by ___ between ___ and ___

A

peptide bonds
carboxylic acid and amino group

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12
Q

complete polypeptides have distinct ends called ___ and ___

A

N terminus (free amino group)
C terminus (free carboxylic acid)

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13
Q

glycine

A

G

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14
Q

alanine

A

A

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15
Q

valine

A

V

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16
Q

leucine

A

L

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17
Q

isoleucine

A

I

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18
Q

proline

A

P

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19
Q

phenylalanine

A

F

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20
Q

tryptophan

21
Q

methionine

22
Q

serine

23
Q

threonine

24
Q

cysteine

25
tyrosine
Y
26
asparagine
N
27
glutamine
Q
28
lysine
K
29
arginine
R
30
histidine
H
31
aspartic acid
D
32
glutamic acid
E
33
selenocysteine
U
34
pyrrolysine
O
35
amino acids with uncharged polar R groups
serine (S), threonine (T), cysteine (C), tyrosine (Y), asparagine (N), glutamine (Q) "n****s quack cause they yell softly"
36
amino acids with basic R groups
lysine (K), arginine (R), histidine (H) "kill rich hoes"
37
amino acids with acidic R groups
aspartic acid (D), glutamic acid (E) "dick everyday"
38
amino acids with non polar R groups
glycine (G), alanine (A), valine (V), leucine (L), isoleucine (I), proline (P), phenylalanine (F), tryptophan (W), methionine (M)
39
genetic alteration substituting one amino acid for another
missense mutation
40
what mutation causes sickle cell disease
glu --> val substitution (V256D)
41
structure level of the amino acid sequence
primary
42
the characteristic geometry of localized regions
secondary structure
43
secondary structure has ___ or ___ geometry
alpha helix or beta pleated sheets
44
___ interactions cause secondary structure
backbone
45
the completed three-dimensional arrangement of a polypeptide
tertiary structure
46
___ interactions cause tertiary structure
R group
47
quaternary structure results in ___
multimeric proteins
48
multimeric proteins are complexes of ___
polypeptide subunits
49
one gene, one enzyme concept is not ___, one gene, one ___ is more accurate
broad enough polypeptide