Chapter 9 Transport In Plants Flashcards

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1
Q

Give 3 reasons why transport system is needed in plants?

A

1) to match metabollic demands in all cells (from roots to leaves)
2) to transport to potentially huge sizes of plant (like tall trees ) need effiecent shstems to move up and down
3) SA:V is not constant in a plant, good at leaves but nit in trunks roots etc, where only simple diffusion would not be effeicent enough to sustain metabollic demands

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2
Q

Explain the first reason “each cell needs to be able to have access to all sushatnces for metabollic demands” (3 you can say)

A
  • each cell needs a certsin amount of substance to carry out metabollic demand. Leaves are able to make glucose and oxygen but the roots cant, so a transport system is needed here, and for waste products tinbe removed (they cant donit).
  • Or roots absorb mineral ions and these need to be transported to every cell, so proteins and enzymes can be made in cell structure etc
  • even normal hormones that are made in ine part of the plant may need to go to the other part and so a transport system is needed here
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3
Q

Deacribe the structure of a leaf in a plant in layers
Where are the stomata
What is mesophyll?
Why are spongy mesophyll cells needed?

A
  • waxy cuticle
  • upper epidermis
  • palsaide (mesophyll) cells
  • spongy (mesophyll ) cells
  • lower epidermis (here the stomata with guard cells are)
  • waxy cuticle too

2) mesophyll refers to middle of leaf
3) spongynis important as these create air spaces which allows for more surface area for gas exchange to take place in to the cells that will photosynthesise

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4
Q

What is a dicotyledonous plant

What is a cotyledon

A

A dicot is a plant that makes seeds that contain two cotyledons
- these are organs that act as food stores for thr developing embryo plant and form the first leaves of the plants when they germinate

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5
Q

What are the two types of dicots and which one do we need to know about (what sre basic features that distinguish them)

A

Need to know about HERBACEOUS DICOTS!
- sift tissues which die at thr end of the growing season

woody dicotd (aborescent)
- hard lignifnied tissues and a long life cycle
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6
Q

How are the vascular bundles in herbaceous dicots arranged differently in stems leaves and roots?

A
  • first in general the xylem is the inner more and phloem outer more

1) stem = on the outside in a ring like fashion to give support
2) roots = in the middle star shaped
3) leaves = midrib of a dicot leaf is the main vein carrying the vascular tiddue , but there are also many small branching veins spread through the leaf for transport and support

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7
Q

Explain why the vascualr bundle is where it id in roots stem leaf

Speaking of veins in leaf how dontou describe this

A
  • in stem around outside in ring like to give support
  • in roots in middle in star shape, and this helps withstand TUGGING STRAINS that resultnas the stems and leaves are blown in the wind
  • in the leaves just ij the midrib as the main vein , this acts as support and provided structure to leaf. With many veins branching out that help in support and function, it also acts as support too.

2) VENATION IS NETTED RATHER THAN LONG AND NARROW (eudicot )

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8
Q

Some basic differences between dicots and monocots

Main featured first

A
  • Dicotd = two cots vs one
  • Venation in dicot is netted vs straight and narrow
  • Petals in dictonim in 4s or 5s compared to 3s in monocots

(You know vb in stem is rung on outside for support, however in stem of monocot scattered)
(You kniw vb in roots is star like in the middle for support, but in monocot in ring )

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9
Q

Function and structure of xylem

  • vessels and how they made
  • in between? What does this have
  • (what is tannin)
  • where does water leave
A

Function = to transport water and mineral ions one way upwards + support of the plant

Structure =

  • made up of several types of dead cells that form the xylem vessels
  • these have no end cell walls and then cells fused to make a continous hollow tube
  • these also are STRENGTHENED by lignin secondary walle for extra meschnical strentgh
  • in between vessels are xylem PARENCHYMA which store food and contain tannin
  • tannin is bitter chemical thwt protects plants from herbivores
    2) watwr leaves through non lignified pits
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10
Q

Function of phloem

A

Function : to tranport food in the form of orgsnic solutes around thr plant from lesves where they are made by photosythenis

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11
Q

Features and structure of phloem

What extra do they have , what reduces resistance and obstruction

A
  • transport can be both up or down
  • made from living cells also joined end to end to form a continous hollow tube , NO LIGNIN
  • in between each end wall the end walls have perfoatednholes called sieve tube plates, this REDUCES RESISTANCE of solute movement making it easier to go
  • most of cell components are broken down and lost to REDUCE OBSTRUCTION
  • as a resukt there are often COMPANION cells attached to a sieve tube element with a cinnected plasmidesmata to allow for rapid transfer of substance. This cell carriees out all life functions for itself and the sieve tube element
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12
Q

Similarities and differences quick between xylem phloem

A
Xylem = dead, phloem alive 
Xylem = end to end walls to make hollow, phloem = yes
Xylem = no nuclues , phloem too
Xylem = lignin = phloem = no
Xylem = one way , phloem = both ways 
Xylem = no end walls , phloem = perfosted holes in them
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13
Q

How to observe vascular bundle particularly xylem in tissue?

More on celery= which is longtidunal but rhubarb which was trnasverse

A

1) for example cellery stem cut horizontally to make as thin circles as possible (vb should be on outside )
2) leave in water then stain in TOLUIDINE BLUE
3) rinse srain and put to slide with water
- yiu should be able to see both xylem (inner ) and phloem

But for rhubarb

1) lick a few out and lush apart , add a drop of kethylene bluen
- get excess out, put drop of glycerol snd view
- here yiu should seenthem straight lines woth rings (this is the lignin!)

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14
Q

Why is water so importsnt for plants

- metabolism / function + structure

A

STRUCTURE AND METABOLLISM

Structure

  • tugor pressure provides a hydrostatic skeleton to support the stems and leaves
  • DRIVES EXPANSION , ALLOWS ROOTS TO FORCE WAY THROUGH TARMAC AND CONCRETE

Function + metabolism

  • helps carry mineral ions
  • cools plant down due to having high latent heat when evaporates
  • needed for photosynthesis
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15
Q

Water enters the plant through the root hair cells, how are they adapted for this? (4)

A

They have root haire which is a long thin extension on the cell

  • these are microscopic size means they can penetrate easily between soil particles
  • each has large SA:V and thousands on each cell
  • thin surfsce layer (just cell wall and membrane), small distance = effeicent diffusion
  • HIGH CYTOPLASMIC SOLUTE CONCENTRATION in cell ensured perfect potential bradient
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16
Q

How do the root hair cells maintsin this low water potential on the inside? (2)
Explain by water potnials and concentration of soluted in bith media still

A
  • they use active transport in proton pumps to create this and ACTIVE TRANSPORT of mineral ions into the soil makes this lower
  • also vacuole takes a lot of the water which lowers water potenial of the cell

As soil has low solute conc, the water potential of it will be high and water thus koves in by osmisis

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17
Q

Once the water has moved into the root hair cell what happens nexr?

A

Water can then move from cell to cell to the xylem in two ways

  • apoplast (cellulose )
  • sympkast (cytoplasm)
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18
Q

Describe the symplast pathway to the xylem

What is soil solution

A

Symplast= simple = cytoplasm
- soil solution moves through a CONTINOUS cytoplasm of cells that is connected through the plasmodesmata by osmosis

  • due to the next cell having a lower water potential , water moves from high to low this way snd keeps moving cell by cell until it reaches xylem
  • the fact that once water oeaves the root hair cell water potenial is low again which maintains steep conc gradient between spil and cell —> water can then continue to move in by osmosis.

2) soil solutionnis mixture of everything dissolved in water until thr endodermis

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19
Q

Describe apoplasy pathway to the xylem

A
  • movement of soil solution through intracellular spaces of cellulose cell wall
  • solution fills these spaces up
  • as water is puller into the xylem the pull from this and the Cohesive forces creates a tension that pulls the water across the cellulose fibres
  • the fibres offer no resistance and so water can move, by apoplast
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20
Q

What happens once the water reaches close to the xylem (endodermis)

Why is this so necessary

A

1) water has moved by both apoplast and symplast cell by cell until it reaches a band of cells surrounding the xylem and phloem called the ENDODERMIS
2) Endodermis has a band called the CASPARIAN STRIP- this is a band of waxy material called SUBERIN that forms a waterproof layer around the endodermis
3) water coming by the APOPLAST pathway is blocked by the casparian strip and thus water is forced to join the SYMPLAST pathway
4) in doing so it passes throiugh selectively permeable cell surface membranes, which blocks any POTENTIALLY TOXIC SOLUTES FROM REACHING LIVING TISSUE

Now plant is protected from any danger, if not there accumlated so much over time and cause diseasE

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21
Q

What else does the endodermis ensure?

A

It ensured any solutes that have accumulated into thr xylem dont go back into the water solution and get wasted.

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22
Q

How does water enter the xylem for the first time (mechanism)
Apoplast or symplast?

What does active pumping of solute create? When is this useful etc snd what else

A
  • the cytopladm of endodermal cells have low solute concentration anyways but
  • solutes are actively pumped in towards the xylem
  • this means xylem has low water potenial but endodermal high
    ==and this increases the amount of osmosis happening and water through
  • at this point its still just SYMPLAST

This creates root pressure and this gives water a bit of s push to the xylem - HOWEVER THIS IS NOT THE MAIN FACTOR OF WATER MOVING TO THE XYLEM.. It is independent of transpiration and can be thought as a helping factor for when say in the night when trsnspirstion stops something still needs to help push the water up .

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23
Q

What FOUR piece of evidence shows a role of active transport for creating root pressure?

A

1) poisons like cynaide that affect the mitochondria (enzymes ) required for respiration put in soil—> no ATP could be made so no active transport could happen —> root pressure fell
2) root pressure increases with a rise of tempertaure and falls with a lower —> suggesting chemical process (and thus active) is involved (like ensyme optimum reaching)
3) A fall of oxygen or respiratory substances equalled a fall in root pressure
4) GUTTANATION happening

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24
Q

What is guutation again and how does it prove active transport used in root pressure

A

This is when xylem sap isnforced oit of ends of leaves. Usually happens at night as water cant escpae due to stomata being closed. Due to a build up of pressure water has to be forced out, but as no transpiration is happening an ACTIVE PROCESS must be happening to push water up agaisnt gravity…

25
Q

Recap how water enters xylem

A
  • root hair cells specialised + active transport of solutes in creates water potential gradient and water moves in
  • moves by symplast and apoplast cell by cell till reach casparain strip at endodermis
  • this forces apoplast to join symplast due to waxy suberin however this means no toxic solutes enter
  • both symplast now to move in xylem . This done by transpiration pull but also ROOT PRESSURE
  • here active transport of solutes into xylem creates gradient such that water moves in , independent of transpiration and evidence profes this (guttaion cynaide temp respiratoy susbatnces)
26
Q

What is the process of transpiration

Why are some stomata open at night too?

A
  • in order to do photosynthesis a plant needs carbon dioxide and let out oxygen produced ss waste product
  • on the underside of leaves stomata controlled by guard cell open in the day to let gas eschange due to gradients
  • however water from cells have been evaporated into vapour which collects in gaps of spongy mesophyll, when stomata open, due to gradient they move out too
  • as a result transpiration is the loss of water from leaves and stem and an inevitable
    consequence of gas eschnsge
  • night time they should be closed as no ohoto happening , but respirstion still shooens so for oxygen tk come in some stomata need to be open . Less water lost at night
27
Q

What is the transpiration stream?

A

Movement of water from roots to xylem to the leaf where it evaporates and leaves by stomata

28
Q

How does transpiration stream carry water then from ground up? (Working backwards now)
1) how does water get pulled from top of xylem

A

1) water molecules evaporate from surfsce of mespohyll cells and mofe into the air spaces in the lesf snd move out of stomata when open by diffusion down conc grsdiejt
2) this loss of water in that cell lowers water potential. Water from adjacent cell moves into that cell by osmosis as a result both pathways
3) this happens cell by cell of leaf to top of xylem, here water moves out of xylem by osmosis into cells of leaf

29
Q

How does transpiration stream carry water then from ground up? (Working backwards now)
2) from bottom of xylem to top = explaom cohesion tension theory

A
  • water form hydrogen bonds with each other (attracted to each other = cohesion) and with the walls of the xylem = adhesion
  • the combined affects of cohesion and adhesion allow water to exhibit cappilary action in a narrow tube . This is how water can move up agaisnt the force of gravity in a tube , as water is lost in the leaf water is pulled up to replace it
  • the loss if water due to transpiration creates tension that causes water to be pulled up, and this helps move water into the roots too. Combine thisnwith capillary actionnand water moves up
  • the model of water moving from soil in a continous stresm upmthe xylem and across the leaf is cohesion tension theory (idea of both cohesion and tension needed)
30
Q

What evidence is there for cohesion tension (3)

A

1) changes of diameter .
- in day when transpiration is at highest there is most tension so the diameter of trees SHRINKS AS IT IS PULLED IN. However wt night when transpiration is lowest diamter IS GREATEST AS TENSIOJ LOWEST SO IT CAN EXPAND = evidence for tension
2) cutting stem makes air come in rather than water going out= due to coehsion of water ?
3) if xylem vessel is cut water can no longer go up as air has just entered and ruined continous stream of water (less cohesion adhesion )

31
Q

Advantages of transpiration

A

Fresh supply if water brings more mineral ions

Evaporation cools it down

32
Q

How to investigate transpiration?

Why is water uptake a good subsitute for measuring trasnpiration?

A

Use potomwter add a bubble and see how it moves in distance/ time
- this is because 99% of water by a plant is lost by transpiration, the extra lost is negligible

33
Q

How do guard cells control the stomata

Tugor pressure, and shape / adapation of the guard cell causing it to change shape assymetrically?

A
  • tugor controlled
  • when conditions favorubale guard cells get solutes pumped in by active transport, causing tugornpressure to increase
  • when conditions no longer favrouable hormonal signals from roote can trigger tugor loss in the guard cells

As guard cells have thick inner cell walls and thin on the outside , with CELLULOSE rings , in high tugor they expand lengthways and the pore opens (outer stretches more)

When lowe tugor it closes as it doesnt contract , assymetric configroustion

34
Q

Factors affecting transpiration rate? (5)

A
Tempertaure
Humidty 
Wind movement
Light intensity 
Soil water availibility
35
Q

How does temp affect transpiration (2)

A

1) temperature
- increased temp increases evaporation and so more water leaves due to kinetic energy increase
- but also increase temp decreases humidity on outisde .
Both inc concentration gradient and thus rate of transpiration

36
Q

How does light intensity affect transpiration

A

Inc light intensity = opens more percentage of stomata (due to photosytheneis) = more transpiration loss

However this only happens to a limit, past this all the stomata are open and increasing light intensity doesnt increase rate of transpiration anymore

37
Q

Humidity affect transpiration

What is humidity

A

Inc humidity= inc amount of conc of water molecules outside the leaf = decrease conc gradient = decrease rate of transpiration

Opposite for dry

Humitiy is measuee of water concentration compared to max water it can hold

38
Q

Air movement transpiration

Soil water availibiltiy.

A

More air movement = higher conc gradient = more transpiration
2) more water in soil = more water loss, less water in soil= less water loss and more stomata closed anyways

39
Q

What is translocation

What are the features of it and what is an assimilate + main one

A

The transport of assimilates in the phloem from sources to sinks

  • this is active process that requires energy
  • can be both up and down
  • organic molecules are the products of photosythesis, and these are called ASSIMILATES
  • the main assimilate is sucrose even thiugh glucose is made in photo
40
Q

What can be sources and sinks

Whats thr conc like at sources and sinks?

A

Sources are like leaves and stems green

  • can be storage irgans like tubers or tap roots in growing period
  • food stores in SEEDS WHEN GERMINATING

sinks

  • tubers fruits taproots
  • meristem tissue actively dividing

2) conc is always lower at sink so it csn go there, here this is controlled by enzymes that make this (like making sucrose into starch thus reducing the conc of sucrose still)
- roots growing

41
Q

Why is sucrose more commonly transported rather than glucose?

A

Thid because sucrose is not uded im metabolism as readily as glucose so LESS LIKELY TO BE METABOLISED DURING THE TRANSPORT PROCESS

42
Q

What is the theory proposed for translocation?

A

Thisnis thr mass flow hypothesis

43
Q

Explain roughly how the mass flow hypothesis works

Do it from the source loading (how can this be done what ways) to unloading at sink and how this causes transport

A
  • first sucrose is loaded into the phloem : this happens actively (active loadig) in the APOPLAST ROUTE but also passively in the SYMPLAST ROUTE
  • now sucrose at the source phloem, water potential decreases here due to more solute putting in and thus water moves IN By osmosis from the xylem / companion cells. THIS INCREASES PRESSURE HERE
  • AT THE SAME TIME at the sink phloem unloading is happening by diffusion, here sucrose leaves the phloem to cells. This reduces sucrose conc, so increases water potenial. As a result water moved out of phloem to companion/ xylem vis osmosis, DECREASING PRESSURE HWRE

FLOW
- as a result a PRESSURE GRADIENT IS MADE WHERE THE GRADIENT PUSHES THE WATER WITH SOLUTES DOWN OR UP DEPENDING WHERE THE LOW PRESSURE IS due to tugor pressure forcing sap that way . This causes MASS FLOW of assimilated to the SINKS.

44
Q

In what ways can assimilated be loaded?

A

Passive way by symplast route

Active way by apoplast route

45
Q

How is phloem loaded via passive symplast route

A

Moves through the cytoplasm of the mesophyll cells on ontonthe sieve tubes by DIFFUSION through the PLASMODESMATA

46
Q

How does phloem loading happen via apoplast route ( where is the active and where is the passive)

A

This is the route where it can go throough the (cell walls) and inter cellular spaces diffusiing into the sieve tube element, but first actively pumped in

Essentially sucrose actively pumped into the companion cells by surrounding tissue, then goes by diffusion into the sieve tube elements from the companion cell (via cell wall thus apoplasy’. This is done by proton pumps:

47
Q

How does the proton pumps work in transporting sucrose into companion cell

A

1) ATP is used to transport a H+ ion via a proton pumo carrier protein from companion to tissue AGAINST CONCENTRATION GRADIENT (here ATP attach, change shape, hyrdolisis change shape again etc)
2) Now there is a high concentration of H+ ions outside companion then inside, and this makes an electrochemical gradient, energy made here is used to cotransport sucrose AGAINST ITS CONC GRADIENT through a cotransporter protein.
3) sucrose goes from low to high into the cell, and now H+ used again. Here this sucrose move by diffusion apoplast route to the sieve tube element, which creates pressure, or done by active too.

48
Q

What happens when phooem unloads at sink

A

Remember how enzymes ensure there always is a lower conc of assimilate at the sink (such as converting glucose to starch etc). This means assimilate moves in to sink BY DIFFUSION

doing so here inc water potential again due to less solute in phloem. As a result water leaves by osmosis back to the xylem and rejoins transpiration stream

49
Q

Summaries translocation

A
  • active loading and passive (apoplast / symplast ) route of assimilate happens. For active proton pumped put by ATP carrier, electrochemical give energy for sucrose to be cotransported in, then goes by diffusion via cellulose hence apoplast
  • this lowers water potential in phloem, so water moves in from xylem / companion INCREASING PRESSURE
  • at sink phloem unloads by simple diffusion as enzyme ensures low conc of assimilate at sink . This inc water potenial so water moves out to xylem via osmosis, lowering pressure.
    # this jus created a PRESSURE GRADIENT causing mass flow of assimilate from high pressure to low pressure due to tugor forcing
50
Q

IMPORTANT how are companion cells adapated for translocation (2)

A

Highly folded membrane to inc surface area for more actove transport
Contains insane mitochondria that provides atp needed for active transport

51
Q

What evidence is there for active loading and mass flow (4)

A
  • microscopy allows us to see specific adpatations on companion cells (fold + mitochondria)
  • mitochondria posioned = no translocatiom (no atp no active loading et)
  • flow of sugars is 10k faster than if diffusiom suggesting an ACTIVE PROCESS NEEDED
  • aphids show this - pressure pushes sap through stylet not the aphid, and because of this you can measure rates at different areas (and see pressure higher at source vs sink) = mass flow theory .
52
Q

What happens when a ring if bark removed from tree? (Important )

A

Xylem is in inside of tree whereas phloem on outside

  • if remove outside transpiration calm but now flow of sugars cant happen. Above the region is fine below its dead, if whole bark is removed fhen tree will die as assimialtes wojt be able to go to roots
  • bark above SWELLS as assimlate accumulates here cauijg low water potenial and water move!!!
53
Q

What is the significance of relationship between diffusion and SA:V on large plants

A

Large olants have large leaves of high SA:V and here more water leaves due to transpiration thus ADAPTATIONS NEEDED (significance)

54
Q

How to calculate rate of diffusion with side lentgh

A

Side lentgh/2 , distance bas ri be to centee of cube ragher fhan whole lentgh of side as you assuming diffusion occurs across whole cube from one side when it isnt ?

55
Q

Difference active vs faccilaiifed doffudion

A

Active vs passive agaisnt vs along

56
Q

How to do potometer set up

A

1) cut stem underwater at a slant
- this prevents air from entering xylem and surface area
2) set up apparatus underwater to so no air
3) USE VASELINE TO DEAL ANY GAPS MAKING AIRTIGHT BETWEEN
4) insert shoot into appartus underwater make sure everythin air tight
5) dry leaves so no gradient
6) remove capiliary tube for a split second ti insert water bubble
7) close screw fix
8) allow climatisatiom
9: reset bubble

57
Q

How to prevent air

A

1) cut underwater + setup underwater + insert plant underwater
2) tightly secure jojns with rubber bung or vaseline
3) remove potometer
4) ensure apparatus full fo water + reservoir so yiu can restart

58
Q

Apparatus

A
1) cut shoot under water 
2; cur shoot at a slant
3) set apparatud up underwater + insert plant in it underswatwe 
4) check it has no air bubble and airtight with casekine erc 
5) dry leaves
6) keep other conditiosn constant 
7) keep ruker fixed and recoed 
7) allow time for climitide