Chapter 9 -Nutrition Flashcards
Name a non-reducing sugar
Sucrose
Name the reducing sugars
Glucose & multose
Describe the test for reducing sugars
Use 4 cubic centimetres of blue benedict’s solution in a test tube
- shake gently to obtian blue colour
- Crush the food to be tested (larger surface area)
- Shake thoroughly
- Heat the content in a waterbath
- An orange/red precipitate indicates a large amount of glucose/multose
- Greenish yellow indicates a small amount
- If the solution remains blue, no reducing sugar is present
Describe the test for non-reducing sugars
- Crush the food to be tested
- Add a small amount of hydrochloric acid to the food
- Boil it for one minute in a water bath
- Neutralise the solution by adding sodium hydrogen carbonate to the test tube
- Carry out the normal benedict’s test
- Evaluate the same
Describe the test for starch
- Crush the food
- Spread it on a white tile
- Add iodine solution (yellow brown) with a syringe
- If the colour changes to black, starch is present
Describe the test for proteins
- Crush the food and place in a test tube
- Add blue Biuret solution
- Mix with a small amount of distilled water
- Shake gently
- If a purple colour appears, proteins are present
Describe the test for fats
- Crush the food & place in test tube
- Add 10 cubic centimetres of ethanol
- Shake thoroughly and leave for 10 minutes
- Add a few drops of distilled water
- The fat forms tiny globules
- A milky solution forms
- If the solution stays clear, no lipids are present
Describe the test for the PRESENCE of vitamin C
- Put blue DCPIP in a test tube
- Add fruit juice (or liquidised food) with a pipette
- Add the fruit juice drop by drop to the DCPIP
- Count the number of drops until the solution goes clear
- If the solution goes clear, vitamin C is PRESENT
Describe an experiment to investigate the relative concentration of vitamin C in different fruit juices
- Basically repeat the presence test for both juices, recording the amount of drops for each. Shake the solution after each drop. Be sure to use the same amount of DCPIP for both experiments. The solution which required the least drops to go clear indicates a higher concentration of vitamin C.
- obviously moet jy dit nou nie so gaan skryf nie, maar julle is mos slim. Ek hoef regtig nie weer die hele vitamin C ding the gaan herhaal nie, although ek nou al so baie nonsens gepraat het ek kon eintlik maar
Name the four main elements present in organic substances
- Carbon (Most important)
- Oxygen
- Hydrogen
- Nitrogen
Define nutrients
Substances with food value which make up food that can be utilised in the metabolic processes of an organism
Define nutrition
The ability of an organism to obtain organic and inorganic nutrients from the environment, which contain energy and raw materials, for growth and development
Describe autotrophic nutrition
Uses inorganic molecules and an external energy source to build up organic molecules. Occurs in plants during photosynthesis
Describe heterotrophic nutrition
Takes organic molecules into the body during nutrition, which are used as a source of energy and growth. Occurs in animals
Name the chemical elements present in carbohydrates
Carbon
Hydrogen
Oxygen
State the ratio in which hydrogen and oxygen is present in carbohydrates
2:1