chapter 20 Flashcards
what are two microscopic, single celled organisms
bacteria and archea
what does prokarytoes mean
before a nucleus
no membrane bond organelles
who discovered prokaryotes, what is referred as?
Dutch microscopist Antonie van Leeuwenhoek
father of microbiology
what did Antonie van Leeuwenhoek study?
what did he also do?
green charophyte alga Spirogyra, Dental Plaque
made microscopes
Prokaryote Structure
Lack a membrane-bounded nucleus (DNA in nucleoid region)
Outer cell wall
Some move by means of flagella
Lack membranous organelles
May have accessory rings of DNA (plasmids)
capsule
gel-like coating outside the cell wall
made up of a polysaccharide layer
called glycocalyx.
fimbriae
hairlike bristles that allow
adhesion to surfaces
nucleoid
location of the
bacterial chromosome
conjugation pilus
elongated, hollow appendage
used to transfer DNA to other cells
flagellum
rotating filament that propels the cell
what kind of DNA do prokaryotic cells have?
have a circular DNA`
Louis Pasteur
devised an experiment that showed that a previously sterilized broth cannot become cloudy with microorganism growth unless it is exposed directly to the air where bacteria are abundant.
Disproved the theory of spontaneous generation of microbes by performing these types of experiments.
Pasteur’s Experiment
HYPOTHESIS A: Bacteria arise spontaneously in a broth
HYPOTHESIS B: Bacteria in the air contaminate a broth
Reproduction in Prokaryotes
Asexual
Prokaryotes reproduce asexually by means of binary fission.
Generation time is as short as 12 minutes
mutations in prokaryotes
are generated rapidly and passed on to offspring more quickly than eukaryotes
are Prokaryotes haploid or diploid
Mutations are immediately subjected to natural selection
Conjugation
Conjugation pilus forms between two cells
Donor cell passes DNA to recipient cell through the pilus
Transformation
Occurs when bacterium picks up free pieces of DNA from other prokaryotes
Becomes incorporated into genome
Transduction
Occurs when bacteriophages carry portions of bacterial DNA from one cell to another
Serve as vectors
most common type of prokaryote
how many
bacteria
Over 9,000 different bacteria have been named (more are being named)
how do bacteria arise
from the division of preexisting bacteria—not by spontaneous generation
what do the cell wall of the bacteria contain?
peptidoglycan
how are bacteria commonly differentiated
through a gram stain procedure
Gram-negative bacteria
have a second plasma membrane which blocks antibiotic drugs, making infections difficult to treat
three basic shapes of a bacteria
Spiral (spirilli),
Rod (bacilli), and
Round (cocci)
Bacterial Metabolism
Obligate aerobes – unable to grow in the absence of free oxygen (need oxygen)
Obligate anaerobes – unable to grow in the presence of free oxygen
Examples: Botulism, gas gangrene, and tetanus
Facultative anaerobes – able to grow in either the presence or absence of free oxygen
vectors
suitcases of holding genetic info to other places and spread
Autotrophic Bacteria
Photoautotrophs
Chemoautotrophs