Chapter 2

Question 1

The introduction of a bacterial sample into media to

culture microbes

Answer 1

inoculation

Question 2

Media that encourages the growth of some

organisms but suppresses the growth of others

Answer 2

Selective Media

Question 3

This media causes an observable change (color or change in pH) in the
medium when a particular biochemical reactions
occurs

Answer 3

Differential Media

Question 4

Media that contains special nutrients that allow growth
of a particular organism that might not otherwise
be present in sufficient numbers.

Answer 4

Enrichment Media

Question 5

A temperature-controlled chamber to encourage the

multiplication of microbes

Answer 5

Incubator

Question 6

A macroscopic cluster of cells appearing on a solid
medium arising from the multiplication of a single
cell

Answer 6

Colony

Question 7

Method commonly used to achieve colony isolation

Answer 7

Streak Plate Technique

Question 8

This lens forms the initial image of the specimen

called the Real Image

Answer 8

Objective lens

Question 9

This lens forms the Virtual Image, which is the
magnified image that is received by the retina and
perceived by the brain.

Answer 9

Ocular lens

Question 10

The capacity of an optical system to distinguish or
separate between two adjacent objects or points
from one another

Answer 10

Resolution

Question 11

Refers to the degree of contrast between an

specimen and its surroundings

Answer 11

Contrast

Question 12

Cells are suspended in water or broth to maintain

viability and provide space for locomotion.

Answer 12

Wet mount prep (ex. Hanging drop slide)

Question 13

Occurs when the slide is passed through an open

flame several times.

Answer 13

Heat Fixation

Question 14

Purpose of heat fixation

Answer 14

1. Kills the microbe
2. adheres the microbe to the slide
3. breaks down the membrane for better stain retention

Question 15

_____ dyes have a positive charge

Answer 15

Basic

Question 16

______dyes have a negative charge

Answer 16

Acidic

Question 17

Dye sticks to the specimen and gives it color

Answer 17

Positive staining technique (requires a basic dye)

Question 18

Dye does not stick to the specimen but settles some

distance from its outer boundary within the background

Answer 18

Negative staining technique (requires an acidic dye)

Question 19

Uses two differently colored dyes: the primary dye
and the counter-stain to distinguish cell types or
parts

Answer 19

Differential Staining Technique

Question 20

Causes all the cells in the smear to appear more or
less the same color, used to
Reveal shape, size, and arrangement

Answer 20

Simple Staining Technique

Question 21

Different results in the Gram stain are due to
differences in the structure of the _________ and
how it reacts to the series of reagents applied to the
cells

Answer 21

Cell Wall

Question 22

Originated as a method to detect Mycobacterium

tuberculosis

Answer 22

Acid Fast Staining

Question 23

Stain distinguishes between spores and vegetative

cells

Answer 23

Endospore Stain

Question 24

Proper order for Gram Staining

Answer 24

Crystal Violet, Iodine, Alcohol Rinse and Safranin

Question 25

Used to emphasize cell parts that are not revealed by conventional staining methods

Answer 25

Special Stain

Question 26

Gram positive cells are stained

Answer 26

purple

Question 27

Gram negative cells are stained

Answer 27

pink

Question 28

Acid fast positive cells are stained

Answer 28

pink

Question 29

Acid fast negative cells are stained

Answer 29

blue