Chapter 10 Flashcards
What question did Griffith ask?
How do certain types of bacteria cause pnuemonia
What did Griffith do to test his question?
He isolated two different strains of pneumonia bacteria from mice and grew them in his lab
What observations did Griffith make about his bacteria cultures?
That the disease causing strain’s colony had smooth edges and the harmless strain’s colony had rough edges
What was Griffith’s 4th experiment?
Griffith mixed his heat killed, disease causing bacteria with live, harmless bacteria and injected the mixture into the mice. The mice developed pneumonia and died.
What did Griffith call the process that took place in his 4th experiment?
Transformation
Define transformation
When one strain of bacteria permanently changes into another
What did Griffith hypothesize after he discovered transformation?
That a factor must contain information that could change harmless bacteria into a disease-causing one
What did Avery do and why?
He repeated Griffith’s last experiment to determine which molecule was most important for transformation
What did Avery make an extract from and what did he do with it?
Heat killed bacteria that they treated with enzymes that destroy proteins, lipids, carbs, nucleic acid, and DNA. Transformation still occurred.
What did Avery repeat Griffith’s experiment using and what was their conclusion?
They repeated his experiment using enzymes that would break down DNA. Transformation didn’t occur, so they concluded that DNA was the transforming factor.
What is a virus that infects bacteria known as?
A bacteriophage
Who was Fredrick Griffith?
A British scientist in the 1920s
What did Hershey and Martha Chase study?
Viruses
What is a bacteriophage composed of?
DNA or an RNA core and a protein coat
What did Hershey and Martha Chase grow the viruses with?
They grew the viruses in cultures containing radioactive isotopes of Phosphorus-32 (32P) and Sulfur-35(35S)
Why did Hershey and Martha Chase grow the viruses with 32P and 35S?
Because if 35S was found in the bacteria, it would mean that the viruses’ protein had been injected into the bacteria. If 32P was found in the bacteria, then it was the DNA that had been injected.
Was 35S or 32P found in the bacteria?
Nearly all the radioactivity in the bacteria was from phosphorus (32P)
What did Hershey and Martha Chase conclude from their experiment?
They concluded that the genetic material of the bacteriophage was DNA, not protein
What is DNA made up of?
Nucleotides
What are nucleic acids made up of?
A 5-carbon sugar, a phosphate group, and a nitrogenous base
What is a nucleotide made up of?
A monomer of nucleic acids
What are the 4 kinds of bases in DNA?
Adenine, Thymine, Guanine, and Cytosine
What did Chargaff discover? What is this discovery called?
That the percentages of Guanine and Cytosine are almost equal and the percentages of Adenine and Thymine are almost equal. These are called Chargaff’s rules
What did Rosalind Franklin use to get information about the structure of DNA?
X-ray diffraction
How did Rosalind Franklin use x-ray beams in her research?
She aimed an X-ray beam at concentrated DNA samples and recorded the scattering pattern of the X-rays on film
Who used clues from Franklin’s pictures/ pattern?
Watson and Crick
How did Watson and Crick use Franklin’s research?
They used clues from her pattern to build a model that explained how DNA carried information and could be copied
What did Watson and Crick’s model look like?
A double helix, in which two strands were wound around each other
What did Watson and Crick discover?
That hydrogen bonds can form only between certain base pairs- adenine and thymine, and guanine and cytosine
What is the theory that only A&T and C&G can bond together?
Base pairing
Who discovered base pairing?
Watson and Crick
What does chromatin contain?
DNA tightly coiled around histones
What does DNA and histone form?
Chromatin
Where is chromatin found?
In eukaryotic chromosomes