Ch 7 Flashcards
Chemotoph - chemoautotroph
Energy source: chemical
Carbon source: co2
Chemotroph - chemo heterotroph
Chemical energy source
Organic compounds
phototroph- photoautotroph
Light source energy source
Aerobic cellular respiration
Glycolysis
Transition step
Respiratory step
Molecular oxygen is the final electron acceptor of etc
Carbon is released as co2
Additional uses of electron transport chain
Movement of flagella
Active transport system
Electron transport chain varies
Both aerobic & anaerobic is possible
Fermentation
Breaking down glucose via glycolysis
Does not require oxygen
There is no kreb cycle & no electron transport chain
Used when respiration is not an option
Fields 2 ATP
NADH is created
amphibolic pathways
Metabolic pathways used in both anabolism and catabolism
Anabolism (bio synthesis)
Requires an input of energy
Includes the synthesis of carbohydrates proteins, lipids, and nucleic acids using precursor monomers from existing catabolic pathways
The end products of fermentation
Can help identify the bacteria and can be commercially valuable
Gram staining
Crystal violet primary stain—-Iodine mordant—Alcohol decolorizer—- Safranin counter stain
Making a bacterial smear an heat fixing a slide
Aseptically transfer broth bacterial culture to a clean slate allowed to air dry 5 to 10 minutes
Heat fixed slides by moving slides through the picture of flame 23 times
Never heat fixed sweat like
The purpose is of heat fixing is to kill and immobilize bacteria on the side
Serial dilution
Are used to create decreasing concentrations of the original sample
When is better dilutions into an iPad for an 11 to go you look for colony forming units between 30 colonies and 300 colonies are accountable
Viber plate count
Account available or lifestyles
Dilute the sample through a series of delicious cultivation
Put 9 mL of Nadiaa and Epdi test tubes one through four
Take one milliliter from the original bacteria faces to two and so on and so forth