Ch 7 Flashcards

1
Q

Chemotoph - chemoautotroph

A

Energy source: chemical
Carbon source: co2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Chemotroph - chemo heterotroph

A

Chemical energy source
Organic compounds

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

phototroph- photoautotroph

A

Light source energy source

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Aerobic cellular respiration

A

Glycolysis
Transition step
Respiratory step
Molecular oxygen is the final electron acceptor of etc
Carbon is released as co2

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Additional uses of electron transport chain

A

Movement of flagella
Active transport system

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Electron transport chain varies

A

Both aerobic & anaerobic is possible

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Fermentation

A

Breaking down glucose via glycolysis
Does not require oxygen
There is no kreb cycle & no electron transport chain
Used when respiration is not an option
Fields 2 ATP
NADH is created

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

amphibolic pathways

A

Metabolic pathways used in both anabolism and catabolism

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Anabolism (bio synthesis)

A

Requires an input of energy
Includes the synthesis of carbohydrates proteins, lipids, and nucleic acids using precursor monomers from existing catabolic pathways

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

The end products of fermentation

A

Can help identify the bacteria and can be commercially valuable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Gram staining

A

Crystal violet primary stain—-Iodine mordant—Alcohol decolorizer—- Safranin counter stain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Making a bacterial smear an heat fixing a slide

A

Aseptically transfer broth bacterial culture to a clean slate allowed to air dry 5 to 10 minutes
Heat fixed slides by moving slides through the picture of flame 23 times
Never heat fixed sweat like
The purpose is of heat fixing is to kill and immobilize bacteria on the side

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Serial dilution

A

Are used to create decreasing concentrations of the original sample
When is better dilutions into an iPad for an 11 to go you look for colony forming units between 30 colonies and 300 colonies are accountable

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Viber plate count

A

Account available or lifestyles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Dilute the sample through a series of delicious cultivation

A

Put 9 mL of Nadiaa and Epdi test tubes one through four
Take one milliliter from the original bacteria faces to two and so on and so forth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Results of expressed colony forming units per milliliter

A

CFU over milliliter

17
Q

Dilutions calculation

A

volume transferred over total volume

18
Q

What are the two common methods of inoculating plates for viable counts?

A

Spread plate method and pour plate
Both message you serial dilution’s as a first step today with the original sample in a series of dilutions s

19
Q

Gene regulation

A

Transcription, post transcriptions, translation, post translation

Only done when necessary

20
Q

Operons

A

Genes with related functions that are transcribed together into One mRNA transcript
Controlled by 1 promoter
all turned on or off together
Found in bacteria, archaea & in a few eukaryotic
This mRNA transcript is called polycistronic

21
Q

DNA promoter

A
22
Q

DNA operator

A

Regulatory DNA region where gene repressor or activator cm bind

23
Q

Lac operon

A

Inducible ( default is off )
Makes enzyme to break down glucose
Turns on when there are lactose enzyme presents

24
Q

Aerobic cellular respiration

A

Glycolysis
Transition step & kreb’s cycle/ tricarboxylic acid
Respiratory chain or election transport chain

25
Q

Electron transport chain of prokaryotes varies tremendously

A

Even single species can have several alternate carriers this allows prokaryotes to grow in many different growth conditions.