Ch. 10 Classification of Microorganisms

Question 1

taxonomy

Answer 1

the science of classifying organisms

Question 2

taxon

Answer 2

category

Question 3

systematics

Answer 3

phylogeny (evolutionary links between species)

Question 4

insert card here for

Answer 4

table 10.1

Question 5

taxonomic hierarchy

Answer 5

grouping species in increasingly general groups.

Domain -> Kingdom (eukarya only) -> Phylum -> Class -> Order (-ales) -> Family (-ace) -> Genus -> Species

Question 6

taxonomy mnemonic

Answer 6

dear king philip, come over for good spaghetti

Question 7

are viruses classified?

Answer 7

yes! though they are not considered living, viral species are classified by their physical characteristics.

Question 8

classification methods

Answer 8

1) staining
2) biochemical tests
3) serology
4) phage typing
5) fatty acid profiles
6) molecular biology

Question 9

staining indicates…

Answer 9

• morphology (rod, cocci, vibrio)
• differential stains (e.g. gram stain)
• special stain (capsule, endospore, flagellum, acid-fast stain)

Question 10

biochemical tests

Answer 10

• can organisms ferment lactos?
• are they facultative anaerobes?
• use a key to figure out the organism
• usually noted by a colour change

Question 11

enteropluri tube

Answer 11

able to do multiple biochemical tests at the same time

Question 12

serology

Answer 12

tests antigen-antibody interactions

- identifies proteins (all bacteria) and tychoic acids (gram-positive)

Question 13

phage typing

Answer 13

• bacteria are colonized on Petri dishes
• drops of different phages on a grid pattern;
• if plaque grows = all bacteria are killed

Question 14

fatty acid profiles

Answer 14

FAME - cellular or outer membrane

Question 15

molecular biology

Answer 15

1) sequences of bases in DNA (DNA microarray)

2) fluorescent rxns with base pairs of oligoneucleotides (FISH)