Cell Signalling 1 Flashcards
What are the 3 membranes which carbohydrates are located on?
- Plasma
- Nuclear
- Mitochondrial
What is the choline R group when attached to a phospholipid?
CH2, CH2, N+ (CH3)3
What is the serine R group when attached to a phospholipid?
CH2, C (H)(COO-)(NH3+)
Why must we ingest two fatty acids?
They cannot be made endogenously and they give rise to arachidonic acid which is involved in cell signalling.
What are 6 effects that we need from signalling?
- Growth
- Differentiate
- Divide
- Secrete and release
- Die
- Store/ mobilise energy
What are the 5 steps to communicating via extracellular signalling?
- Synthesis and release of the signalling molecule by the signalling cell
- Transport of the signal to the target cell
- Detection of the incoming signal by a specific binding protein (receptor) at the target cell
- Binding of the signal to the receptor generates a biological response within the target cell
- Removal of signal - termination
What are the 4 types of signal?
Hormones and growth factors
Metabolic Regulators
Neurotransmitters
Inflammatory Mediators
What are the major types of signalling based on distance?
- Endocrine signalling (secretion of hormones into the bloodstream)
- Paracrine signalling (signalling to adjacent cells
- Autocrine signalling (signalling back onto the same cell)
What are the approx distances for each type of signalling?
Autocrine - few metres
Paracrine - few micrometres
Autocrine - same cell
As most signalling molecules are membrane impermeable, what must they bind to?
Cell surface receptors in the form of integral membrane proteins
What are the 3 major classes of membrane receptors?
Ionotrophic (ligand gated ion channels that allow ions to bind in order to open the channel.
Metabotrophic (7 transmembrane spanning G-protein couples)
Catalytic (tyrosine kinases linked e.g. insulin, nerve growth factor receptors)
Give 2 examples of effector enzymes and the 2nd messenger molecules they activate
Adenylyl cyclase - adenosine cyclic monophosphate (cAMP)
Phospholipase C - DAG
What is a G - protein?
A molecular switch which carries information to effector enzymes. They are active when bound to GTP and inactive when bound to GDP.
What subunit of G - proteins binds to GTP and becomes activated?
Alpha subunit
What happens with GTP binds to the G- protein?
It dissociates, releasing the free active alpha sub unit. Reassociates when GTP is hydrolysed to GDP byt a GTPase activity.