Cell Signalling 1 Flashcards

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1
Q

What are the 3 membranes which carbohydrates are located on?

A
  • Plasma
  • Nuclear
  • Mitochondrial
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2
Q

What is the choline R group when attached to a phospholipid?

A

CH2, CH2, N+ (CH3)3

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3
Q

What is the serine R group when attached to a phospholipid?

A

CH2, C (H)(COO-)(NH3+)

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4
Q

Why must we ingest two fatty acids?

A

They cannot be made endogenously and they give rise to arachidonic acid which is involved in cell signalling.

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5
Q

What are 6 effects that we need from signalling?

A
  • Growth
  • Differentiate
  • Divide
  • Secrete and release
  • Die
  • Store/ mobilise energy
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6
Q

What are the 5 steps to communicating via extracellular signalling?

A
  1. Synthesis and release of the signalling molecule by the signalling cell
  2. Transport of the signal to the target cell
  3. Detection of the incoming signal by a specific binding protein (receptor) at the target cell
  4. Binding of the signal to the receptor generates a biological response within the target cell
  5. Removal of signal - termination
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7
Q

What are the 4 types of signal?

A

Hormones and growth factors
Metabolic Regulators
Neurotransmitters
Inflammatory Mediators

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8
Q

What are the major types of signalling based on distance?

A
  1. Endocrine signalling (secretion of hormones into the bloodstream)
  2. Paracrine signalling (signalling to adjacent cells
  3. Autocrine signalling (signalling back onto the same cell)
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9
Q

What are the approx distances for each type of signalling?

A

Autocrine - few metres
Paracrine - few micrometres
Autocrine - same cell

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10
Q

As most signalling molecules are membrane impermeable, what must they bind to?

A

Cell surface receptors in the form of integral membrane proteins

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11
Q

What are the 3 major classes of membrane receptors?

A

Ionotrophic (ligand gated ion channels that allow ions to bind in order to open the channel.
Metabotrophic (7 transmembrane spanning G-protein couples)
Catalytic (tyrosine kinases linked e.g. insulin, nerve growth factor receptors)

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12
Q

Give 2 examples of effector enzymes and the 2nd messenger molecules they activate

A

Adenylyl cyclase - adenosine cyclic monophosphate (cAMP)

Phospholipase C - DAG

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13
Q

What is a G - protein?

A

A molecular switch which carries information to effector enzymes. They are active when bound to GTP and inactive when bound to GDP.

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14
Q

What subunit of G - proteins binds to GTP and becomes activated?

A

Alpha subunit

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15
Q

What happens with GTP binds to the G- protein?

A

It dissociates, releasing the free active alpha sub unit. Reassociates when GTP is hydrolysed to GDP byt a GTPase activity.

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16
Q

What are the 7 steps to G protein coupled receptor signalling to effector enzymes?

A
  1. Hormones binds to receptor
  2. G - protein (GDP bound) associates with receptor
  3. GTP/GDP exchange on G - protein (GTP bound)
  4. G - protein dissociates into into GTP bound alpha and a beta-gamma subunit
  5. A subunit (with GTP bound) activates effector enzyme
  6. Effector enzyme produces 2nd messenger
  7. GTP hydrolysed to GDP, G-protein complex reassociates and signalling ends
17
Q

What is the purpose of the GPCR signalling producing a GTP bound alpha subunit? What causes this reaction to stop?

A

Causes cAMP from ATP to be produced leading to a cascade reaction.
Stopped by when GDP binds to alpha subunit and it can no longer fir the binding site.

18
Q

What happens with cAMP binds to another PKA?

A

A conformational change so cAMP breaks apart and exposes the catalytic part.
This can then phosphorylate proteins.

19
Q

How many units does PKA have? After breakdown, what subunit can take a phosphate group and phosphorylate serine?

A

4, C subunit

20
Q

What is the process of phosphodiesterase?

A

The degradation of cAMP by PKA by turning it to 5AMP. cAMP can then not bind to PKA so the signal ends.

21
Q

What stimulates PKA? What can PKA then do once stimulated?

A

cAMP - PKA can then phosphorylate some proteins or inhibit glycogen synthesis.

22
Q

What effector can both increase and decrease cAMP concentration?

A

Adenylyl cyclase