Cell Cycle Flashcards

1
Q

what is cell proliferation ?

A
  • cell grows + divides to produce 2 daughter cells
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2
Q

what are the three main types of cells (growth) ?

A
  • labile cells
  • stable cells
  • permanent cells
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3
Q

what are labile cells + example ?

A
  • constantly dividing
  • epithelial cells
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4
Q

what are stable cells + example ?

A
  • low rate of division normally but can rapidly divide in response to injury or stimuli.
  • eg. hepatocytes
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5
Q

what are permanent cells ?

A
  • permanently differentiated so unable to divide
  • eg. neurons
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6
Q

what is an example of illness with decreased proliferation and increased cell death ?

A
  • chronic wound
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7
Q

what is an example of illness with increased proliferation and decreased cell death ?

A
  • cancer
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8
Q

what are the 3 stages of the cell cycle ?

A
  • Interphase
  • Mitosis (nuclear division)
  • Cytokinesis (cytoplasm division)
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9
Q

what are the stages of interphase ?

A
  • G1 phase (gap 1 )
  • S Phase
  • G2 phase
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10
Q

what happens during the G1 phase ?

A
  • cell grows before DNA synthesis
  • synthesizes proteins + organelles
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11
Q

what happens during the S Phase ?

A
  • DNA replication
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12
Q

what happens during the G2 Phase ?

A
  • after DNA synthesis, lasts until cell division
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13
Q

what is G0 phase ?

A
  • cells can enter G0 from G1 if conditions aren’t favourable
  • resting phase
  • do not divide
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14
Q

what cells are permanently in the G0 Phase ?

A
  • nerve cells
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15
Q

what are the 5 Phases of Mitosis ?

A
  • prophase
  • prometaphase
  • metaphase
  • anaphase
  • telophase
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16
Q

what happens during prophase ?

A
  • chromosomes condense
  • nucleolus disappears
  • centrioles move to opposite poles
17
Q

what happens during prometaphase ?

A
  • spindle fibers / micro tubules attach to centromere
  • chromosomes fully condense
18
Q

what happens during metaphase ?

A
  • chromosomes align at equator
19
Q

what happens during anaphase ?

A
  • centromere divides
  • sister chromatids are pulled to opposite poles
20
Q

what happens during telophase ?

A
  • nuclear membrane reforms
  • chromosomes decondense
21
Q

what happens during cytokinesis ?

A
  • cytoplasm divides
  • 2 daughter cells produced
22
Q

what is the role of G1 checkpoint ?

A
  • checks if cell is large enough
  • if environment is favourable
  • checks DNA for damage
23
Q

what is the role of the
G2 checkpoint ?

A
  • checks duplicated DNA for any damage
  • checks cell size
24
Q

what is the role of the M (metaphase) checkpoint ?

A
  • checks all chromosomes are aligned and attached to spindle fibers
25
Q

How CDK’s (Cyclin Dependent Kinases) activated ?

A
  • Cyclin binds to CDK exposing its active site
  • protein substrate and ATP bind to CDK active site
  • protein substrate is phosphorylated
  • phosphorylated protein regulates cell cycle at different stages
26
Q

Phosphorylation of Rb protein?

A
  • mitogens activate cyclin D
  • Cyclin D binds to CDK 4/6
  • CDK 4/6 phosphorylates Rb protein
  • Rb protein is attached to E2F (transcription factor)
  • phosphorylation of Rb inactivates it
  • E2F is released and switches on cell cycle