C7 DNA to Protein Flashcards
What is a promoter
Location of the initiation of synthesis
Three steps in Nucleic acid polymerization
Initiation, elongation, termination
Bacterial RNAP uses this to help recognize the DNA sequence of a promoter
sigma factor
Has polycistronic DNA (eukarya vs prokarya)
prokarya
In prokaryotes, it directs RNAP to start at different promoter sequences
sigma factor
number of RNA polymerases in prokaryotes
1
number of RNA polymerases in eukaryotes
3
Which RNAP makes rRNA in eukaryotes
pol I
Which RNAP makes tRNA and snRNA in eukaryotes
pol III
Which RNAP makes mRNA in eukaryotes
pol II
Requires multiple general transcription factors to form a transcription initiation complex
RNAP 2
The TBP region of this transcription factor binds to the TATA box within the promoter
TFIID
What occurs when RNAP II transitions from initiation to elongation?
Initiation factors release and C-terminal domain of RNAP is phosphorylated
Why does thymine in DNA have less error than Uracil in RNA?
cytosine can be deaminated to uracil. Easily recognized in DNA as error. More difficult in RNA.
In eukaryotes, transcription occurs here
nucleus