C10 Manipulating genes and cells

Question 1

Most animal cells cannot grow indefinitely due to the loss of ?

Answer 1

telomerase

Question 2

Most animal cells cannot grow indefinitely with the exception of ?

Answer 2

embryonic stem cells

Question 3

molecular scissors for DNA

Answer 3

Restriction enzymes

Question 4

“dideoxy” method developed by..

Answer 4

Sanger

Question 5

southern blotting procedure

Answer 5

electrophoresis of DNA cut with restriction enzymes. blot gel onto nitrocellulose. allow labeled DNA probe to hydridize to specific sequence on nitrocellulose.

Question 6

? hybridization can locate genes on chromosomes

Answer 6

in situ

Question 7

? are commonly used as cloning vectors

Answer 7

bacterial plasmids

Question 8

cDNA

Answer 8

DNA created by reverse transcription of mRNA

Question 9

Problem with cDNA library

Answer 9

produces more DNA for those genes transcribed more often

Question 10

in vitro

Answer 10

in glass

Question 11

site-directed mutagenesis

Answer 11

synthetic primer containing desired mutated sequence binds to nearly complementary sequence. When duplicated, two different DNA sequences are created.

Question 12

Single stranded DNA sequence used in hybridization to detect certain sequences

Answer 12

DNA probe

Question 13

joins DNA fragments together to produce a recombinant DNa molecule

Answer 13

ligase

Question 14

Causes most common type of hemophilia

Answer 14

factor 8

Question 15

most common was to rapidly sequence DNA

Answer 15

dideoxy sequencing

Question 16

regulatory regions upstream of coding region can be made to control expression of a ? whose expression can be easily monitored

Answer 16

reporter gene