BOTANY LAB: MICROSCOPE Flashcards
Greek word ‘micro’ - meaning Small and ‘skopion’ - meaning to See or Look
MICROSCOPE
A machine for looking at small things
MICROSCOPE
Used to look at the anatomy of small organisms such as insects, the fine structure of rocks and crystals, or individual cells.
MICROSCOPE
Proper Care and Maintenance of Microscope
- Handle with care
- Look after lenses
- Keep covered
- Store safely
- Keep clean/ Clean carefully
- Take care of bulbs
- Refers to manual
- Annual maintenance check
- Consider a professional service
PARTS OF THE MICROSCOPE AND ITS USES
- Eyepiece
- Eyepiece tube
- Coarse adjustment knob
- Arm
- Revolving nosepiece
- Objective lens
- Stage
- Stage clips
- Illuminating mirror
- Base
MOUNTING OF SPECIMEN ON MICROSCOPIC SLIDE
- The specimen is placed directly on the slide. A cover slip may be used to keep the specimen in place and to help protect the objective lens.
- This is suitable for specimens such as Samples of pollen, Hair, Feathers, or Plant materials
DRY MOUNT
MOUNTING OF SPECIMEN ON MICROSCOPIC SLIDE
- Made by placing a fluid solution on a slide, suspending a specimen in a solution, and then covering the specimen and the solution with a cover slide.
WET MOUNT
The number of times an object is enlarged by a lens system or enlarged in the drawing or illustration.
MAGNIFICATION
Even the lowest power objectives such as x4 provide too much magnification for some specimens which cannot be encompassed within the field of view.
REDUCTION
Computing for Magnification
Magnification = V ocular x V objective
Magnification = Size of Image/ Size of Object
MAGNIFICATION LENS
- 4x
SCANNING
MAGNIFICATION LENS
- 10x
LPO
MAGNIFICATION LENS
- 40x
HPO
MAGNIFICATION LENS
- 100x
OIO
Magnification of Ocular lens…
10x
