Biolab finals

Question 1

Are proteins that catalyze or accelerate chemical reactions.

Answer 1

Enzyme

Question 2

The molecules at the beginning of the process of acceleration and catalyzing, these are called?

Answer 2

Substrates

Question 3

It is when the enzyme converts them into different molecules

Answer 3

The products

Question 4

Enzymes can be (?) and (?) with salts, solvents and other reagents

Answer 4

Denatured and precipitated

Question 5

Enzymes can be Denatured and precipitated with (?), (?) and (?)

Answer 5

salts, solvents and other reagents

Question 6

(?) can be Denatured and precipitated with salts, solvents and other reagents

Answer 6

Enzymes

Question 7

Enzymes have molecular weights ranging from (?) to (?)

Answer 7

10,000 to 2,000,000

Question 8

Many enzymes require the presence of other compounds - cofactors - before their catalytic activity can me exerted. this entire active complex is referred to as the (?)

Answer 8

holoenzyme

Question 9

Many enzymes require the presence of other (?) - (?) - before their catalytic activity can me exerted. this entire active complex is referred to as the holoenzyme

Answer 9

compounds - cofactors

Question 10

Apoenzyme + cofactor = ?

Answer 10

Holoenzyme

Question 11

(?) + (?) = Holoenzyme

Answer 11

Apoenzyme + cofactor

Question 12

A non-protein organic substance which is dialyzable, thermostable and loosely attached to the protein part

Answer 12

Coenzyme

Question 13

An organic substance which is dialyzable and thermostable which is firmly attached to the protein or apoenzyme portion

Answer 13

Prosthetic group

Question 14

The enzyme will catalyze only one reaction

Answer 14

Absolute specificity

Question 15

The enzyme will act only on molecules that have specific functional groups, such as amino, phosphate and methyl groups.

Answer 15

Group Specificity

Question 16

The enzyme will act on a particular type of chemical bond regardless of the rest of the molecular structure

Answer 16

Linkage specificity

Question 17

The enzyme will act on a particular steric or optical isomer

Answer 17

Steriochemical specificity

Question 18

Are polymers of high molecular weight with nucleotides as the repeating unit.

Answer 18

Nucleic acids

Question 19

Are mostly conjugated with proteins to form nucleoproteins

Answer 19

Nucleic acids

Question 20

Nucleic acids are mostly (?) with proteins to form nucleoproteins

Answer 20

conjugated

Question 21

Nucleic acids are mostly conjugated with proteins to form (?)

Answer 21

nucleoproteins

Question 22

In most living organisms (exept for viruses), genetic information is stored in the molecule (?)

Answer 22

deoxyribonucleic acid or DNA

Question 23

Is made and resides in the nucleus of living cells

Answer 23

DNA

Question 24

DNA Is made and resides in the (?) of living cells

Answer 24

nucleus

Question 25

Four different nucleotide bases occur in the DNA:

Answer 25

Adenine (A), Cytosine (C), Guanine (G), Thymine (T).

Question 26

It gets it's name from the sugar group in the molecule's backbone ribose

Answer 26

RNA

Question 27

Has a sugar phosphate backbone with nucleotide bases attached to it.

Answer 27

RNA

Question 28

Like DNA, RNA contains the bases (?), (?) and (?)

Answer 28

Adenine (A), Cytosine (C), Guanine (G)

Question 29

RNA does not contain?

Answer 29

Thymine (T).

Question 30

RNA does not contain Thymine (T), but instead, RNA's fourth nucleotide is the base (?)

Answer 30

Uracil (U)

Question 31

RNA is a (?) stranded molecule

Answer 31

Single stranded molecule

Question 32

DNA is a (?) Stranded Molecule

Answer 32

Double stranded

Question 33

RNA is the main genetic material used in the organisms called (?)

Answer 33

Viruses

Question 34

(?) is the main genetic material used in the organisms called viruses

Answer 34

RNA

Question 35

RNA can move around the cells of living organisms and thus serves as a sort of (?)

Answer 35

Genetic messenger

Question 36

DNA was first prepared from (?) and (?)

Answer 36

thymus or muscle tissues

Question 37

(?) was first prepared from thymus or muscle tissues

Answer 37

DNA

Question 38

RNA was first isolated from (?)

Answer 38

yeast

Question 39

(?) was first isolated from yeast

Answer 39

RNA

Question 40

what is the color formed in the test for nucleoproteins?

Answer 40

Purple color

Question 41

In the test for nucleoproteins, what and how many drops were administered?

Answer 41

5-10 drops of 1% CuSO4 (Copper Sulfate)

Question 42

What color was formed in the isolation of RNA from yeast

Answer 42

Brownish solution

Question 43

What is the heat required in the isolation of RNA from yeast and for how long?

Answer 43

heat for 90 degrees celsius for 30 minutes

Question 44

What color was formed in the Test for presence of phosphates

Answer 44

Yellow color

Question 45

what percentage and amount of sulfuric acid was administered to remaining filtrate? (Test for phosphates)

Answer 45

20ml of 10% H2SO4 (Sulfuric Acid) to remaining filtrate

Question 46

1 ml of acid hydrolysis + bial orcinol’s reagent

Answer 46

Yellow color

Question 47

1 ml of 0.1% ribose solution + bial orcinol’s reagent

Answer 47

Blue-green color

Question 48

0.1% glucose solution + bial orcinol’s reagent

Answer 48

Yellow brown color

Question 49

What color was formed in the test for presence of purines

Answer 49

White color

Question 50

In the test for presence of purines, how many ml and percentage of ammonium hydroxide were administered

Answer 50

3 ml of 10% NH4OH (ammonium hydroxide)

Question 51

color formed in the BIURET TEST

Answer 51

Violet color

Question 52

TEST FOR CATALASE ACTIVITY how many ml of extract?

Answer 52

5 ml of extract

Question 53

Color formed in the TEST FOR CATALASE ACTIVITY

Answer 53

Blue to green coloration

Question 54

TEST FOR SPECIFICITY OF ENZYME ACTION

Question 55

In the TEST FOR SPECIFICITY OF ENZYME ACTION, test tube 1. Yellowish to orange hue (indicates that the starch had already been metabolized)

Answer 55

(indicates that the starch had already been metabolized)

Question 56

In the TEST FOR SPECIFICITY OF ENZYME ACTION, test tube 2. For the first five minutes, it has an extreme starch that contains a (?)

Answer 56

bluish black

Question 57

Urine will containe (?) and other excess (?) from your body

Answer 57

Acetone, ketones

Question 58

Acetone bodies: 5% ammonium sulfate solution + 5% sodium nitroprusside solution + Conc. Ammonium hydroxide. What color was formed after adding the NH4OH, and what is in the middle?

Answer 58

It turned yellow after adding NH4OH and the middle is a red color which shows presence of ketone

Question 59

Acetone bodies: What does the color red mean?

Answer 59

shows the presence of ketone

Question 59

In the urine Benedict's reagent, what color did the urine change into from yellow?

Answer 59

It changed to green or greenish-yellow color

Question 60

In creatinine, what color did the urine change into from yellow?

Answer 60

It changed into orange-red

Question 61

What was the result/color in the test for pathological constituents?

Answer 61

Blue solution

Question 62

In the test for normal constituents, what color was formed?

Answer 62

Light blue

Question 63

In the test for normal constituents, how many ml and percentage of sodium carbonate solution was administered?

Answer 63

2ml of 10% sodium carbonate solution

Question 64

Characteristics of normal urine: The quantity averages to?

Answer 64

The quantity averages to 1500 to 2000ml in an adult man daily

Question 65

Characteristics of normal urine: The specific gravity is?

Answer 65

The specific gravity is 1.010 to 1.025

Question 66

Characteristics of normal urine: The odor is?

Answer 66

The odor is slightly aromantic

Question 67

The reaction of normal urine is slightly acidic with an average of?

Answer 67

pH of 6.0

Question 68

A liquid or product by our body that is secreted by our kidneys and then excretyed through the urethra.

Answer 68

Urine

Question 69

The specific gravity of water is?

Answer 69

1000