Biochem Of Red Cells

Question 1

Hb-O2 curve is what shape?

Answer 1

Sigmoid shape

Question 2

An increase in DPG does what to curve ?

Answer 2

Shift to right

Question 3

An increase in pH does what to curve?

Answer 3

Shift to left

Question 4

Decrease in temp does what to curve?

Answer 4

Shift to left

Question 5

Increase in CO2 does what to curve?

Answer 5

Shift to right

Question 6

Embden- Myerhof pathway?

Answer 6

Anaerobic glycolysis pathway - generates ATP and NADH - reverts Fe+++ (metHb) to Fe++ (Hb)

Question 7

Hexose monophosphate shunt?

Answer 7

Generate NADPH - reduces glutathione

Question 8

Rapapoport- lubesing shunt ?

Answer 8

Generates 2,3 DPG - right shift of curve to allow O2 release

Question 9

What is glutathione?

Answer 9

Tripeptide

Consists of glutamate, cysteine, glycine

Question 10

What are free radicals?

Answer 10

Highly reactive molecules with unpaired electrons

Question 11

What’s so good about reduced glutathione?

Answer 11

Protects against free radicals, detoxifies hydrogen peroxide

Question 12

What is wrong with hydrogen peroxide?

Answer 12

Primary intermediate in oxidative damage

Question 13

How is CO2 transported in the blood?

Answer 13

Bicarbonate ion - facilitated by carbonic anhydrase
Bound to Hb (carbamino haemoglobin)
Physically dissolved in solution