Bio Test 1b Flashcards

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1
Q

alpha helix

A

formed by many hydrogen bonds, lots of space and flexibility

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2
Q

beta sheet

A

formed by many hydrogen bonds, hold sheets together, very strong

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3
Q

denaturation

A

when a material comes in contact with protein and unfolds it

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4
Q

chaperones

A

assist protein folding in the cell

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5
Q

prion proteins

A

special infectious mis-folded proteins, cause scrapie and mad cow

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6
Q

functions of extracellular proteins

A
structural proteins (collagens)
enzymes (lysozyme)
growth factor (fibroblast growth factor)
immune response (antibodies)
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7
Q

functions of membrane proteins

A
transporters (ion channels)
anchors (integrins)
receptors (growth factor receptors)
enzymes (adenylyl cyclase)
recognition (glycoproteins)
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8
Q

functions of intracellular proteins

A
enzymes (catalase)
structural proteins (actin filament, spectrin)
motor proteins (myosin)
receptors (hormone receptors)
signal transduction (kinase)
special functions (DNA binding proteins)
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9
Q

antibody

A

called immunoglobin (Ig), has unique Y shape, tetramer composed of two short chains and two long chains, variable domain on ends of Y where specific antigens bind

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10
Q

centrifugation

A

process by which you take a sample of something and separate its contents by size and density

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11
Q

homogenization

A

plasma membrane of cells can be ruptured so cell contents can be released to create extract

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12
Q

types of homogenization

A

ultrasound
detergent
force cells through small hole
grinding by plunger in tight-fit tube

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13
Q

types of chromatography

A

ion exchange chromatography
gel filtration chromatography
affinity chromatography

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14
Q

ion exchange chromatography

A

separates proteins by charges

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15
Q

gel filtration chromatography

A

separates proteins by size

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16
Q

affinity chromatography

A

separates proteins by the binding capacity to the packed protein