Bio Test 1b Flashcards
alpha helix
formed by many hydrogen bonds, lots of space and flexibility
beta sheet
formed by many hydrogen bonds, hold sheets together, very strong
denaturation
when a material comes in contact with protein and unfolds it
chaperones
assist protein folding in the cell
prion proteins
special infectious mis-folded proteins, cause scrapie and mad cow
functions of extracellular proteins
structural proteins (collagens) enzymes (lysozyme) growth factor (fibroblast growth factor) immune response (antibodies)
functions of membrane proteins
transporters (ion channels) anchors (integrins) receptors (growth factor receptors) enzymes (adenylyl cyclase) recognition (glycoproteins)
functions of intracellular proteins
enzymes (catalase) structural proteins (actin filament, spectrin) motor proteins (myosin) receptors (hormone receptors) signal transduction (kinase) special functions (DNA binding proteins)
antibody
called immunoglobin (Ig), has unique Y shape, tetramer composed of two short chains and two long chains, variable domain on ends of Y where specific antigens bind
centrifugation
process by which you take a sample of something and separate its contents by size and density
homogenization
plasma membrane of cells can be ruptured so cell contents can be released to create extract
types of homogenization
ultrasound
detergent
force cells through small hole
grinding by plunger in tight-fit tube
types of chromatography
ion exchange chromatography
gel filtration chromatography
affinity chromatography
ion exchange chromatography
separates proteins by charges
gel filtration chromatography
separates proteins by size