BCM39/40

Question 1

what is the carbon source in methophile

Answer 1

c1 compounds
no c-c bounds
ch4 ch3oh etc

Question 2

what is cytophaga

Answer 2

bacteria which can degrade cellulose

Question 3

what is pseudomonas

Answer 3

genus with ots of c sources including alchol sugar, benzena and amino acids

Question 4

give examples of obligate aerobes

Answer 4

mycobacterium

azotobacter

Question 5

give an example of anobligate anaerobe

Answer 5

clostridium dificite

Question 6

give an example of a facultive anaerobe

Answer 6

streptococcus salivarius

true fa = e.coli

Question 7

what does super oxide dismutase catalyse

Answer 7

02- -> o2 or h20

Question 8

what is the fenton reaction

and what can it be used for

Answer 8

fe2+ +h2o2 -> fe3+ +ho. +oh-
Fe3+ +h202 -> fe2+ +hoo. +h+
free radicals eg. hoo- are powerful non selecitve oxidants and can oxidise contaminants

Question 9

what is the naming based on

Answer 9

energy source 
- chemotroph
- phototroph
carbon source
-heterotroph
-autotroph
h donor source 
-lithotroph
-organotroph

Question 10

what domain do lithotrophs often belong to

Answer 10

bacteria or archea

often extemophile

Question 11

what are green plants

Answer 11

photolithotrophs

litho as h comes from water

Question 12

what are animals fungi and protists

Answer 12

chemoorgano heterotrophs

Question 13

what is the advantages of a defined medium

Answer 13

• avoid batch variation
• low protein concentration which hinder purity
• eliminates contamination
• elimanate interfereing factos

Question 14

what is the opposite of a defined medium

Answer 14

a complex medium

Question 15

what is the rate of bacteria increase = to

Answer 15

mu x mass of bacteria

Question 16

what is mu = to

Answer 16

lnx-lnx0/t1-t0

mu = (logX -logX)/t-t0 * 2.303

Question 17

how do you calculate the doubling time

Answer 17

0.693 /mu

Question 18

what is the equation for the bacterial growth curve

Answer 18

mu*td/2.303 + logX0 = logX

slope intercept

Question 19

how is cell mass measured in bacteria

Answer 19

od scattered light
photometer measures transmitted
nephelometer measured scattered
ussually at 600nm

Question 20

what is a coulter counter

Answer 20

as particles flow through microchanela nd cause brief change to electrical resistance of lqd.

Question 21

who discovered lithotrophy

Answer 21

winogradsky