BCM39/40 Flashcards
what is the carbon source in methophile
c1 compounds
no c-c bounds
ch4 ch3oh etc
what is cytophaga
bacteria which can degrade cellulose
what is pseudomonas
genus with ots of c sources including alchol sugar, benzena and amino acids
give examples of obligate aerobes
mycobacterium
azotobacter
give an example of anobligate anaerobe
clostridium dificite
give an example of a facultive anaerobe
streptococcus salivarius
true fa = e.coli
what does super oxide dismutase catalyse
02- -> o2 or h20
what is the fenton reaction
and what can it be used for
fe2+ +h2o2 -> fe3+ +ho. +oh-
Fe3+ +h202 -> fe2+ +hoo. +h+
free radicals eg. hoo- are powerful non selecitve oxidants and can oxidise contaminants
what is the naming based on
energy source - chemotroph - phototroph carbon source -heterotroph -autotroph h donor source -lithotroph -organotroph
what domain do lithotrophs often belong to
bacteria or archea
often extemophile
what are green plants
photolithotrophs
litho as h comes from water
what are animals fungi and protists
chemoorgano heterotrophs
what is the advantages of a defined medium
- avoid batch variation
- low protein concentration which hinder purity
- eliminates contamination
- elimanate interfereing factos
what is the opposite of a defined medium
a complex medium
what is the rate of bacteria increase = to
mu x mass of bacteria
what is mu = to
lnx-lnx0/t1-t0
mu = (logX -logX)/t-t0 * 2.303
how do you calculate the doubling time
0.693 /mu
what is the equation for the bacterial growth curve
mu*td/2.303 + logX0 = logX
slope intercept
how is cell mass measured in bacteria
od scattered light
photometer measures transmitted
nephelometer measured scattered
ussually at 600nm
what is a coulter counter
as particles flow through microchanela nd cause brief change to electrical resistance of lqd.
who discovered lithotrophy
winogradsky
