bcm 35 Flashcards
where is the etc?
inner mitochondrial membrane
what are the 2 steps of ox phos?
etc
chemiosomosis
describe complex 1
- name
- stucture
- mechanism
nadh dehydrogenase flavoprotein largest transmembrane protein L shaped steam egninge like movement pumps 2e- from nadh through fmn fe4s4 to uq-> ugh2 4H+ pumped into ims
describe complex 2
name
mechanism
succinate dehydrogenase
delta g is too small for h+ pumping
2e- from fadh goes through fe2s4 cyt b and uq -> ugh2
what inhibits complex 2?
malonate
describe complex 3
cytochrome oxireducatase - cyt b/c complex
runs q cycle
3 subunits - cyt c iron sulfer proteins and cytob
cytochrome c is a mobile protein that attatches to complex 3 on ims
oxides uqh2-> uq
pass 2e- to cytochrome c
pumps 4+ into ims
describe the q cycle
UGH2 in membrane is oxised to ugh. and electron is carried by cyt c(can only carry one at a time)
second uqh2 is then oxidised and 1 e- goes to cyt c and the other regenerates uqh2 from the free radical
describe complex 4
cytochrome c oxidase
cyt c gives 2e- to complex 4
reduce 1/2 O2 to h2o
2H+ is pumped across
inhibitors of complex 4
cyanice
co
azide
describe complex 5
atpase
3H+/at[
1Nadh-> 10H+ -> 2.5 atp
why is an extra proton required for translocation?
movement of atp->ims or adp-> matrix or pi-> matrix or OH->ims changes the ph gradient equivalent to extra proton
describe ubiquinol?
fat soluble redox carrier dissolved in inner membrane
why must semiubiquinol be handeled with care?
ugh. can make superoxide
what can reactive oxygen species cause? give examples
oxidative stress
super oxide
peroxide
hydroxyl radicals
what is lipid peroxidation?
free radical chain reaction that makes fats rancid and so would destroy the mitochondron
