bcm24

Question 1

competative

Answer 1

binds active site

Question 2

non cometative

Answer 2

non active site bound

Question 3

uncompetative

Answer 3

binds enzyme subtrate complex

Question 4

malonate

Answer 4

competative on succinate

Question 5

azt

Answer 5

competativve on hivrt

Question 6

nevirapine

Answer 6

non competative on hivrt

Question 7

sarine

Answer 7

nerve gas
group specifc on ache
destroy active site
bind tightly. permemnant modification

Question 8

aspirin

Answer 8

irreversible inhibitor on cox2 enxyme
acetylates cyclooxygenase cox2 at serine in active site
prevents prostaglandin synthesis.

Question 9

organophosphates

Answer 9

group specific irreversible inhibitors of acetylcholinesterase
adds phosphate to ache irreversible phosphorylation
nerve gas - sarine
insecticides - malathion

Question 10

suicide inhibitors

Answer 10

substrate analogues that are activated by the catalytic cycle of the enzyme

Question 11

suicide inhibitors examples

Answer 11

thymidylate synthase: dUMP->dTMP

5-F- dUMP -> 5 F dUMP destrots active site

Question 12

competative inhibitors

Answer 12

often substrate analogies. like malonate to succinate

Question 13

vmax and km of competatively inhibited enzyme

Answer 13

same vmax

km goes to kapp

Question 14

linearisation of michealessmenton

Answer 14

1/v=km/vmax * 1/[s] + 1/vmax

Question 15

vmax and km of competative inhibiition on lineweaver burk plot

Answer 15

same y intercept means same apparent vm

but higher slope means km increased to km app

Question 16

non competative inhibitor km and vmax

Answer 16

km same

but vmax goes to vmax app

Question 17

lineweaver burk plot of non competative inhibitor

Answer 17

higher y intercept means decreased vmax

same x intercept means same km app

Question 18

are group specific substrate analogues

Answer 18

not neccessarily