BCM30 Flashcards
synthatase
uses atp
synthase
no atp
phosphatase
removes phosphate
phosphorylates
add phosphate
kinase
cataylses transfer of phosphate groups
how is ammonia assimilated
via glutamine synthetase
glutamate + NH3 + ATP ->glutamine
glutamate synthase
alpha keto gluterate -> glutamate
what do transaminases do?
transfer NH2 from aa to alpha keto acids
with pyridoxal phosphaate
swap nh3 for cooh
glutamate + oxaloacetate +glutamate -> alphaketogluterate + aspartate
what is the keq for most transaminases?
1
used both catabolically and anabolically
what do all areas with food production in the world?
1 legume
1 grass
what does glutamate dehydrogenase do?
deaminates glutamate freeing ammonia
glutamate alpha keto gluterate
reductive removal of ammonia from
why cant glutamate dehydrogenase be used to assimilate ammonia in plants?
because the km (NH3) for gs = 10um
and gdh = 20mm
pga transamination
serine
pep transamination
-
pyruvate transamination
alanine
akg transamination
glutamate
oxaloacetate transamination
aspartate
ribose p transamination
-
pga modification of c skel
glycine
cysteine
pep modification of c skel
tyrosine
phenylamine
tryptophan
pyruvate modification of carbon skeleton
valine
leucine
akg modification of carbon skeleton
glutamine
proline
arginine
oxaloacetate modification of carbon skel
aparagine lysine threonine methione isoleucine
ribose p modication of carbon skel
histidine
what does urea look lik
CO(NH2)2
what is the only new enzyme required in the urea cycle
arg->urea
evolved from krebs
not just catabolic also anabolic forms arginine
what happens if excess purine in diet
-> uric acid -> allanine via uricase
primates lack uricase so goes to uric acid which can form crystals and gout
what is allanoin
soluble used in cosmetics
desrcibe ureotellic excretion
urea animals limited water less toxic, highly soluble mammals and sharks*used for osmoreg aatp/n
describe ammoniatellic excretion
ammonia large excess water highly toxic through gills bony fish and most invertebrate - woodlouse
describe uricotellic excretion
uric acid very limited water access insoluble solid birds reptiles insets monotremes dalmations 2atp/n
how do amphibians excrete urea
by simple diffusion
what could have led to rise of the dinosaurs
uricotellic excretion
useful in permian with limited water acess