bacterial metabolism Flashcards

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1
Q

energy source for phototrophs

A

light

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2
Q

energy source for chemotrophs

A

oxidation of inorganic compounds

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3
Q

electron sources for lithotrophs

A

reduced inorganic molecules

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4
Q

electron sources for organotrophs

A

organic molecules

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5
Q

carbon sources for autotrophs

A

CO2 sole or principal biosynthetic carbon source

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6
Q

carbon source for heterotrophs

A

reduced, preformed, organic molecules from other organisms

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7
Q

for heterotrophs organic carbon is the

A

carbon source
- pyrivayte generated as a key molecule through glycolysis. If oxygen is present pyruvate can be oxidised to carbon dioxide. Oxidation is the removal of electrons. these electrons then enter the ETC where the majority of ATP is produced

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8
Q

the calvin cycle allows the

A

further oxidation of pyruvate and the generation of further electrons

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9
Q

denitrification in E.coli used

A

the enzyme nitrate reductase

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10
Q

chemolithotrophy

A

oxidation of inorganic materials- most are autotrophs

e.g. inorganic energy and electron source goes the ETC and ATP is produced

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11
Q

nitrifying bacteria

A

able to grow chemolithotrophicaly at the expense of reduced inorganic nitrogen compounds. Found in alpha, beta, gimme and delta proteobacteria

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12
Q

fermentation

A

if there is not ETC or terminal electron acceptor, the reduced NADH needs to be oxidised back to NAD+ for glycolysis. Pyruvate reduced by NADH
–> substrate level phosophorylation

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13
Q

3 parts of the nitrogen cycle

A

1) nitrogen fixation
2) nitrification
3) denitrification

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14
Q

how much of air is nitrogen

A

78%- key for amino and nucleic acids and important part of ATP

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15
Q

nitrogen fixation

A

anaerobic process in which N2 is reduced to NH3. Bacteria are responsible for this process. Plants cannot use nitrogen in our atmosphere without nitrogen fixing bacteria.

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16
Q

nitrification

A

NH3 is converted to NO2 by nitrifying bacteria and then further to NO3 these can be absorbed and used

17
Q

denitrification

A

nitrates are converted back to atmospheric nitrogen by denitrifying bacteria

18
Q

in the nitrogen cycle nitrogen acts as an

A

electron acceptor

19
Q

ammonia monooxygenase

A

oxidises NH£ to NH2OH

20
Q

nitrite oxidase

A

oxidises NO2- to NO3-

21
Q

reaction in nitrosomonas

A

NH3 + 11/2O2 –> NO2 -+H2O

22
Q

reaction in nitrobacter

A

NO2- + 1/2O2 –> NO3-

23
Q

what is N2 fixation performed by

A

nitrogenase complex

24
Q

two enzymes in the nitrogenase complex

A

dinitrogenase and dinitrogenase reductase

25
Q

nitrogenase is inhibited by

A

oxygen–> aerobic nitrogen fixers protect nitrogenase

26
Q

symbiosis in N2 fixation

A

rhizobia are free living bacteria in the soil that interact with the roots of legumes. A single rhizobia species is generally able to infect certain species of legumes and not others

27
Q

examples of aerobic electron acceptors and their reduced products

A

O2 –> H2O

- all aerobic bacteria, fungi and protists

28
Q

examples of a few anaerobic electron acceptors and reduced products

A

NO3- –> NO2-

SO42- –> H2S

29
Q

nitrosomonas

A

Nitrosomonas is a genus of rod-shaped chemoautotrophic bacteria. This organism oxidizes ammonia into nitrite as a metabolic process. Nitrosomonas are useful in bioremediation.

30
Q

nitrobacter

A

Nitrobacter is a genus comprising rod-shaped, gram-negative, and chemoautotrophic bacteria.